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A mouse model of peripheral nerve injury induced by Japanese encephalitis virus

Japanese encephalitis virus (JEV) is the most important cause of acute encephalitis in Eastern/Southern Asia. Infection with this virus also induces peripheral nerve injury. However, the disease pathogenesis is still not completely understood. Reliable animal models are needed to investigate the mol...

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Autores principales: Wang, Xiaoli, Wang, Guowei, Yang, Huan, Fu, Shihong, He, Ying, Li, Fan, Wang, Huanyu, Wang, Zhenhai
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9731479/
https://www.ncbi.nlm.nih.gov/pubmed/36441775
http://dx.doi.org/10.1371/journal.pntd.0010961
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author Wang, Xiaoli
Wang, Guowei
Yang, Huan
Fu, Shihong
He, Ying
Li, Fan
Wang, Huanyu
Wang, Zhenhai
author_facet Wang, Xiaoli
Wang, Guowei
Yang, Huan
Fu, Shihong
He, Ying
Li, Fan
Wang, Huanyu
Wang, Zhenhai
author_sort Wang, Xiaoli
collection PubMed
description Japanese encephalitis virus (JEV) is the most important cause of acute encephalitis in Eastern/Southern Asia. Infection with this virus also induces peripheral nerve injury. However, the disease pathogenesis is still not completely understood. Reliable animal models are needed to investigate the molecular pathogenesis of this condition. We studied the effect of Japanese encephalitis virus infection in C57BL/6 mice after a subcutaneous challenge. Limb paralysis was determined in mice using behavioral tests, including a viral paralysis scale and the hanging wire test, as well as by changes in body weight. Nerve conduction velocity and electromyography testing indicated the presence of demyelinating neuropathy of the sciatic nerve. Pathological changes in neural tissues were examined by immunofluorescence and transmission electron microscopy, which confirmed that the predominant pathologic change was demyelination. Although Western blots confirmed the presence of the virus in neural tissue, additional studies demonstrated that an immune-induced inflammatory response resulted in severe never injury. Immunofluorescence confirmed the presence of Japanese encephalitis virus in the brains of infected mice, and an inflammatory reaction was observed with hematoxylin-eosin staining as well. However, these observations were inconsistent at the time of paralysis onset. In summary, our results demonstrated that Japanese encephalitis virus infection could cause inflammatory demyelination of the peripheral nervous system in C57BL/6 mice.
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spelling pubmed-97314792022-12-09 A mouse model of peripheral nerve injury induced by Japanese encephalitis virus Wang, Xiaoli Wang, Guowei Yang, Huan Fu, Shihong He, Ying Li, Fan Wang, Huanyu Wang, Zhenhai PLoS Negl Trop Dis Research Article Japanese encephalitis virus (JEV) is the most important cause of acute encephalitis in Eastern/Southern Asia. Infection with this virus also induces peripheral nerve injury. However, the disease pathogenesis is still not completely understood. Reliable animal models are needed to investigate the molecular pathogenesis of this condition. We studied the effect of Japanese encephalitis virus infection in C57BL/6 mice after a subcutaneous challenge. Limb paralysis was determined in mice using behavioral tests, including a viral paralysis scale and the hanging wire test, as well as by changes in body weight. Nerve conduction velocity and electromyography testing indicated the presence of demyelinating neuropathy of the sciatic nerve. Pathological changes in neural tissues were examined by immunofluorescence and transmission electron microscopy, which confirmed that the predominant pathologic change was demyelination. Although Western blots confirmed the presence of the virus in neural tissue, additional studies demonstrated that an immune-induced inflammatory response resulted in severe never injury. Immunofluorescence confirmed the presence of Japanese encephalitis virus in the brains of infected mice, and an inflammatory reaction was observed with hematoxylin-eosin staining as well. However, these observations were inconsistent at the time of paralysis onset. In summary, our results demonstrated that Japanese encephalitis virus infection could cause inflammatory demyelination of the peripheral nervous system in C57BL/6 mice. Public Library of Science 2022-11-28 /pmc/articles/PMC9731479/ /pubmed/36441775 http://dx.doi.org/10.1371/journal.pntd.0010961 Text en © 2022 Wang et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Wang, Xiaoli
Wang, Guowei
Yang, Huan
Fu, Shihong
He, Ying
Li, Fan
Wang, Huanyu
Wang, Zhenhai
A mouse model of peripheral nerve injury induced by Japanese encephalitis virus
title A mouse model of peripheral nerve injury induced by Japanese encephalitis virus
title_full A mouse model of peripheral nerve injury induced by Japanese encephalitis virus
title_fullStr A mouse model of peripheral nerve injury induced by Japanese encephalitis virus
title_full_unstemmed A mouse model of peripheral nerve injury induced by Japanese encephalitis virus
title_short A mouse model of peripheral nerve injury induced by Japanese encephalitis virus
title_sort mouse model of peripheral nerve injury induced by japanese encephalitis virus
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9731479/
https://www.ncbi.nlm.nih.gov/pubmed/36441775
http://dx.doi.org/10.1371/journal.pntd.0010961
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