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Effects and interactions of metal oxides in microparticle‐enhanced cultivation of filamentous microorganisms

Filamentous microorganisms are used as molecular factories in industrial biotechnology. In 2007, a new approach to improve productivity in submerged cultivation was introduced: microparticle‐enhanced cultivation (MPEC). Since then, numerous studies have investigated the influence of microparticles o...

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Detalles Bibliográficos
Autores principales: Laible, Andreas Reiner, Dinius, Anna, Schrader, Marcel, Krull, Rainer, Kwade, Arno, Briesen, Heiko, Schmideder, Stefan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9731605/
https://www.ncbi.nlm.nih.gov/pubmed/36514528
http://dx.doi.org/10.1002/elsc.202100075
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author Laible, Andreas Reiner
Dinius, Anna
Schrader, Marcel
Krull, Rainer
Kwade, Arno
Briesen, Heiko
Schmideder, Stefan
author_facet Laible, Andreas Reiner
Dinius, Anna
Schrader, Marcel
Krull, Rainer
Kwade, Arno
Briesen, Heiko
Schmideder, Stefan
author_sort Laible, Andreas Reiner
collection PubMed
description Filamentous microorganisms are used as molecular factories in industrial biotechnology. In 2007, a new approach to improve productivity in submerged cultivation was introduced: microparticle‐enhanced cultivation (MPEC). Since then, numerous studies have investigated the influence of microparticles on the cultivation. Most studies considered MPEC a morphology engineering approach, in which altered morphology results in increased productivity. But sometimes similar morphological changes lead to decreased productivity, suggesting that this hypothesis is not a sufficient explanation for the effects of microparticles. Effects of surface chemistry on particles were paid little attention, as particles were often considered chemically‐inert and bioinert. However, metal oxide particles strongly interact with their environment. This review links morphological, physical, and chemical properties of microparticles with effects on culture broth, filamentous morphology, and molecular biology. More precisely, surface chemistry effects of metal oxide particles lead to ion leaching, adsorption of enzymes, and generation of reactive oxygen species. Therefore, microparticles interfere with gene regulation, metabolism, and activity of enzymes. To enhance the understanding of microparticle‐based morphology engineering, further interactions between particles and cells are elaborated. The presented description of phenomena occurring in MPEC eases the targeted choice of microparticles, and thus, contributes to improving the productivity of microbial cultivation technology.
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spelling pubmed-97316052022-12-12 Effects and interactions of metal oxides in microparticle‐enhanced cultivation of filamentous microorganisms Laible, Andreas Reiner Dinius, Anna Schrader, Marcel Krull, Rainer Kwade, Arno Briesen, Heiko Schmideder, Stefan Eng Life Sci Reviews Filamentous microorganisms are used as molecular factories in industrial biotechnology. In 2007, a new approach to improve productivity in submerged cultivation was introduced: microparticle‐enhanced cultivation (MPEC). Since then, numerous studies have investigated the influence of microparticles on the cultivation. Most studies considered MPEC a morphology engineering approach, in which altered morphology results in increased productivity. But sometimes similar morphological changes lead to decreased productivity, suggesting that this hypothesis is not a sufficient explanation for the effects of microparticles. Effects of surface chemistry on particles were paid little attention, as particles were often considered chemically‐inert and bioinert. However, metal oxide particles strongly interact with their environment. This review links morphological, physical, and chemical properties of microparticles with effects on culture broth, filamentous morphology, and molecular biology. More precisely, surface chemistry effects of metal oxide particles lead to ion leaching, adsorption of enzymes, and generation of reactive oxygen species. Therefore, microparticles interfere with gene regulation, metabolism, and activity of enzymes. To enhance the understanding of microparticle‐based morphology engineering, further interactions between particles and cells are elaborated. The presented description of phenomena occurring in MPEC eases the targeted choice of microparticles, and thus, contributes to improving the productivity of microbial cultivation technology. John Wiley and Sons Inc. 2021-12-02 /pmc/articles/PMC9731605/ /pubmed/36514528 http://dx.doi.org/10.1002/elsc.202100075 Text en © 2021 The Authors. Engineering in Life Sciences published by Wiley‐VCH GmbH. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ (https://creativecommons.org/licenses/by-nc-nd/4.0/) License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.
spellingShingle Reviews
Laible, Andreas Reiner
Dinius, Anna
Schrader, Marcel
Krull, Rainer
Kwade, Arno
Briesen, Heiko
Schmideder, Stefan
Effects and interactions of metal oxides in microparticle‐enhanced cultivation of filamentous microorganisms
title Effects and interactions of metal oxides in microparticle‐enhanced cultivation of filamentous microorganisms
title_full Effects and interactions of metal oxides in microparticle‐enhanced cultivation of filamentous microorganisms
title_fullStr Effects and interactions of metal oxides in microparticle‐enhanced cultivation of filamentous microorganisms
title_full_unstemmed Effects and interactions of metal oxides in microparticle‐enhanced cultivation of filamentous microorganisms
title_short Effects and interactions of metal oxides in microparticle‐enhanced cultivation of filamentous microorganisms
title_sort effects and interactions of metal oxides in microparticle‐enhanced cultivation of filamentous microorganisms
topic Reviews
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9731605/
https://www.ncbi.nlm.nih.gov/pubmed/36514528
http://dx.doi.org/10.1002/elsc.202100075
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