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Vesicular stomatitis virus-based vaccine targeting plasmodium blood-stage antigens elicits immune response and protects against malaria with protein booster strategy

Merozoite invasion of the erythrocytes in humans is a key step in the pathogenesis of malaria. The proteins involved in the merozoite invasion could be potential targets for the development of malaria vaccines. Novel viral-vector-based malaria vaccine regimens developed are currently under clinical...

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Autores principales: Sun, Yifan, Shi, Xiaodan, Lu, Feng, Fu, Haitian, Yin, Yi, Xu, Jiahui, Jin, Cheng, Han, Eun-taek, Huang, Xuan, Chen, Yongquan, Dong, Chunsheng, Cheng, Yang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9731671/
https://www.ncbi.nlm.nih.gov/pubmed/36504817
http://dx.doi.org/10.3389/fmicb.2022.1042414
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author Sun, Yifan
Shi, Xiaodan
Lu, Feng
Fu, Haitian
Yin, Yi
Xu, Jiahui
Jin, Cheng
Han, Eun-taek
Huang, Xuan
Chen, Yongquan
Dong, Chunsheng
Cheng, Yang
author_facet Sun, Yifan
Shi, Xiaodan
Lu, Feng
Fu, Haitian
Yin, Yi
Xu, Jiahui
Jin, Cheng
Han, Eun-taek
Huang, Xuan
Chen, Yongquan
Dong, Chunsheng
Cheng, Yang
author_sort Sun, Yifan
collection PubMed
description Merozoite invasion of the erythrocytes in humans is a key step in the pathogenesis of malaria. The proteins involved in the merozoite invasion could be potential targets for the development of malaria vaccines. Novel viral-vector-based malaria vaccine regimens developed are currently under clinical trials. Vesicular stomatitis virus (VSV) is a single-stranded negative-strand RNA virus widely used as a vector for virus or cancer vaccines. Whether the VSV-based malarial vaccine is more effective than conventional vaccines based on proteins involved in parasitic invasion is still unclear. In this study, we have used the reverse genetics system to construct recombinant VSVs (rVSVs) expressing apical membrane protein 1 (AMA1), rhoptry neck protein 2 (RON2), and reticulocyte-binding protein homolog 5 (RH5), which are required for Plasmodium falciparum invasion. Our results showed that VSV-based viral vaccines significantly increased Plasmodium-specific IgG levels and lymphocyte proliferation. Also, VSV-PyAMA1 and VSV-PyRON2sp prime-boost regimens could significantly increase the levels of IL-2 and IFN-γ-producing by CD4(+) and CD8(+) T cells and suppress invasion in vitro. The rVSV prime-protein boost regimen significantly increase Plasmodium antigen-specific IgG levels in the serum of mice compared to the homologous rVSV prime-boost. Furthermore, the protective efficacy of rVSV prime protein boost immunization in the mice challenged with P. yoelii 17XL was better compared to traditional antigen immunization. Together, our results show that VSV vector is a novel strategy for malarial vaccine development and preventing the parasitic diseases.
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spelling pubmed-97316712022-12-09 Vesicular stomatitis virus-based vaccine targeting plasmodium blood-stage antigens elicits immune response and protects against malaria with protein booster strategy Sun, Yifan Shi, Xiaodan Lu, Feng Fu, Haitian Yin, Yi Xu, Jiahui Jin, Cheng Han, Eun-taek Huang, Xuan Chen, Yongquan Dong, Chunsheng Cheng, Yang Front Microbiol Microbiology Merozoite invasion of the erythrocytes in humans is a key step in the pathogenesis of malaria. The proteins involved in the merozoite invasion could be potential targets for the development of malaria vaccines. Novel viral-vector-based malaria vaccine regimens developed are currently under clinical trials. Vesicular stomatitis virus (VSV) is a single-stranded negative-strand RNA virus widely used as a vector for virus or cancer vaccines. Whether the VSV-based malarial vaccine is more effective than conventional vaccines based on proteins involved in parasitic invasion is still unclear. In this study, we have used the reverse genetics system to construct recombinant VSVs (rVSVs) expressing apical membrane protein 1 (AMA1), rhoptry neck protein 2 (RON2), and reticulocyte-binding protein homolog 5 (RH5), which are required for Plasmodium falciparum invasion. Our results showed that VSV-based viral vaccines significantly increased Plasmodium-specific IgG levels and lymphocyte proliferation. Also, VSV-PyAMA1 and VSV-PyRON2sp prime-boost regimens could significantly increase the levels of IL-2 and IFN-γ-producing by CD4(+) and CD8(+) T cells and suppress invasion in vitro. The rVSV prime-protein boost regimen significantly increase Plasmodium antigen-specific IgG levels in the serum of mice compared to the homologous rVSV prime-boost. Furthermore, the protective efficacy of rVSV prime protein boost immunization in the mice challenged with P. yoelii 17XL was better compared to traditional antigen immunization. Together, our results show that VSV vector is a novel strategy for malarial vaccine development and preventing the parasitic diseases. Frontiers Media S.A. 2022-11-24 /pmc/articles/PMC9731671/ /pubmed/36504817 http://dx.doi.org/10.3389/fmicb.2022.1042414 Text en Copyright © 2022 Sun, Shi, Lu, Fu, Yin, Xu, Jin, Han, Huang, Chen, Dong and Cheng. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Sun, Yifan
Shi, Xiaodan
Lu, Feng
Fu, Haitian
Yin, Yi
Xu, Jiahui
Jin, Cheng
Han, Eun-taek
Huang, Xuan
Chen, Yongquan
Dong, Chunsheng
Cheng, Yang
Vesicular stomatitis virus-based vaccine targeting plasmodium blood-stage antigens elicits immune response and protects against malaria with protein booster strategy
title Vesicular stomatitis virus-based vaccine targeting plasmodium blood-stage antigens elicits immune response and protects against malaria with protein booster strategy
title_full Vesicular stomatitis virus-based vaccine targeting plasmodium blood-stage antigens elicits immune response and protects against malaria with protein booster strategy
title_fullStr Vesicular stomatitis virus-based vaccine targeting plasmodium blood-stage antigens elicits immune response and protects against malaria with protein booster strategy
title_full_unstemmed Vesicular stomatitis virus-based vaccine targeting plasmodium blood-stage antigens elicits immune response and protects against malaria with protein booster strategy
title_short Vesicular stomatitis virus-based vaccine targeting plasmodium blood-stage antigens elicits immune response and protects against malaria with protein booster strategy
title_sort vesicular stomatitis virus-based vaccine targeting plasmodium blood-stage antigens elicits immune response and protects against malaria with protein booster strategy
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9731671/
https://www.ncbi.nlm.nih.gov/pubmed/36504817
http://dx.doi.org/10.3389/fmicb.2022.1042414
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