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Epitope profiling of monoclonal antibodies to the immunodominant antigen BmGPI12 of the human pathogen Babesia microti
The significant rise in the number of tick-borne diseases represents a major threat to public health worldwide. One such emerging disease is human babesiosis, which is caused by several protozoan parasites of the Babesia genus of which B. microti is responsible for most clinical cases reported to da...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Frontiers Media S.A.
2022
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9732259/ https://www.ncbi.nlm.nih.gov/pubmed/36506011 http://dx.doi.org/10.3389/fcimb.2022.1039197 |
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author | Chand, Meenal Choi, Jae-Yeon Pal, Anasuya C. Singh, Pallavi Kumari, Vandana Thekkiniath, Jose Gagnon, Jacqueline Timalsina, Sushma Gaur, Gauri Williams, Scott Ledizet, Michel Mamoun, Choukri Ben |
author_facet | Chand, Meenal Choi, Jae-Yeon Pal, Anasuya C. Singh, Pallavi Kumari, Vandana Thekkiniath, Jose Gagnon, Jacqueline Timalsina, Sushma Gaur, Gauri Williams, Scott Ledizet, Michel Mamoun, Choukri Ben |
author_sort | Chand, Meenal |
collection | PubMed |
description | The significant rise in the number of tick-borne diseases represents a major threat to public health worldwide. One such emerging disease is human babesiosis, which is caused by several protozoan parasites of the Babesia genus of which B. microti is responsible for most clinical cases reported to date. Recent studies have shown that during its intraerythrocytic life cycle, B. microti exports several antigens into the mammalian host using a novel vesicular-mediated secretion mechanism. One of these secreted proteins is the immunodominant antigen BmGPI12, which has been demonstrated to be a reliable biomarker of active B. microti infection. The major immunogenic determinants of this antigen remain unknown. Here we provide a comprehensive molecular and serological characterization of a set of eighteen monoclonal antibodies developed against BmGPI12 and a detailed profile of their binding specificity and suitability in the detection of active B. microti infection. Serological profiling and competition assays using synthetic peptides identified five unique epitopes on the surface of BmGPI12 which are recognized by a set of eight monoclonal antibodies. ELISA-based antigen detection assays identified five antibody combinations that specifically detect the secreted form of BmGPI12 in plasma samples from B. microti-infected mice and humans but not from other Babesia species or P. falciparum. |
format | Online Article Text |
id | pubmed-9732259 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-97322592022-12-10 Epitope profiling of monoclonal antibodies to the immunodominant antigen BmGPI12 of the human pathogen Babesia microti Chand, Meenal Choi, Jae-Yeon Pal, Anasuya C. Singh, Pallavi Kumari, Vandana Thekkiniath, Jose Gagnon, Jacqueline Timalsina, Sushma Gaur, Gauri Williams, Scott Ledizet, Michel Mamoun, Choukri Ben Front Cell Infect Microbiol Cellular and Infection Microbiology The significant rise in the number of tick-borne diseases represents a major threat to public health worldwide. One such emerging disease is human babesiosis, which is caused by several protozoan parasites of the Babesia genus of which B. microti is responsible for most clinical cases reported to date. Recent studies have shown that during its intraerythrocytic life cycle, B. microti exports several antigens into the mammalian host using a novel vesicular-mediated secretion mechanism. One of these secreted proteins is the immunodominant antigen BmGPI12, which has been demonstrated to be a reliable biomarker of active B. microti infection. The major immunogenic determinants of this antigen remain unknown. Here we provide a comprehensive molecular and serological characterization of a set of eighteen monoclonal antibodies developed against BmGPI12 and a detailed profile of their binding specificity and suitability in the detection of active B. microti infection. Serological profiling and competition assays using synthetic peptides identified five unique epitopes on the surface of BmGPI12 which are recognized by a set of eight monoclonal antibodies. ELISA-based antigen detection assays identified five antibody combinations that specifically detect the secreted form of BmGPI12 in plasma samples from B. microti-infected mice and humans but not from other Babesia species or P. falciparum. Frontiers Media S.A. 2022-11-25 /pmc/articles/PMC9732259/ /pubmed/36506011 http://dx.doi.org/10.3389/fcimb.2022.1039197 Text en Copyright © 2022 Chand, Choi, Pal, Singh, Kumari, Thekkiniath, Gagnon, Timalsina, Gaur, Williams, Ledizet and Mamoun https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Cellular and Infection Microbiology Chand, Meenal Choi, Jae-Yeon Pal, Anasuya C. Singh, Pallavi Kumari, Vandana Thekkiniath, Jose Gagnon, Jacqueline Timalsina, Sushma Gaur, Gauri Williams, Scott Ledizet, Michel Mamoun, Choukri Ben Epitope profiling of monoclonal antibodies to the immunodominant antigen BmGPI12 of the human pathogen Babesia microti |
title | Epitope profiling of monoclonal antibodies to the immunodominant antigen BmGPI12 of the human pathogen Babesia microti
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title_full | Epitope profiling of monoclonal antibodies to the immunodominant antigen BmGPI12 of the human pathogen Babesia microti
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title_fullStr | Epitope profiling of monoclonal antibodies to the immunodominant antigen BmGPI12 of the human pathogen Babesia microti
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title_full_unstemmed | Epitope profiling of monoclonal antibodies to the immunodominant antigen BmGPI12 of the human pathogen Babesia microti
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title_short | Epitope profiling of monoclonal antibodies to the immunodominant antigen BmGPI12 of the human pathogen Babesia microti
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title_sort | epitope profiling of monoclonal antibodies to the immunodominant antigen bmgpi12 of the human pathogen babesia microti |
topic | Cellular and Infection Microbiology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9732259/ https://www.ncbi.nlm.nih.gov/pubmed/36506011 http://dx.doi.org/10.3389/fcimb.2022.1039197 |
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