Mouse kidney nuclear isolation and library preparation for single-cell combinatorial indexing RNA sequencing

Single-cell combinatorial indexing RNA sequencing (sci-RNA-seq3) enables high-throughput single-nucleus transcriptomic profiling of multiple samples in one experiment. Here, we describe an optimized protocol of mouse kidney nuclei isolation and sci-RNA-seq3 library preparation. The use of a dounce t...

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Detalles Bibliográficos
Autores principales: Li, Haikuo, Humphreys, Benjamin D.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9732400/
https://www.ncbi.nlm.nih.gov/pubmed/36595916
http://dx.doi.org/10.1016/j.xpro.2022.101904

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9732400/
https://www.ncbi.nlm.nih.gov/pubmed/36595916
http://dx.doi.org/10.1016/j.xpro.2022.101904

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