Measuring ventral nerve cord stiffness in live flat-dissected Drosophila embryos by atomic force microscopy

Drosophila is an amenable system for addressing the mechanics of morphogenesis. We describe a workflow for characterizing the mechanical properties of its ventral nerve cord (VNC), at different developmental stages, in live, flat-dissected embryos employing atomic force microscopy (AFM). AFM is perf...

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Detalles Bibliográficos
Autores principales: Karkali, Katerina, Jorba, Ignasi, Navajas, Daniel, Martin-Blanco, Enrique
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9732408/
https://www.ncbi.nlm.nih.gov/pubmed/36595903
http://dx.doi.org/10.1016/j.xpro.2022.101901
Descripción
Sumario:Drosophila is an amenable system for addressing the mechanics of morphogenesis. We describe a workflow for characterizing the mechanical properties of its ventral nerve cord (VNC), at different developmental stages, in live, flat-dissected embryos employing atomic force microscopy (AFM). AFM is performed with spherical probes, and stiffness (Young’s modulus) is calculated by fitting force curves with Hertz’s contact model. For complete details on the use and execution of this protocol, please refer to Karkali et al. (2022).

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