Measuring ventral nerve cord stiffness in live flat-dissected Drosophila embryos by atomic force microscopy

Drosophila is an amenable system for addressing the mechanics of morphogenesis. We describe a workflow for characterizing the mechanical properties of its ventral nerve cord (VNC), at different developmental stages, in live, flat-dissected embryos employing atomic force microscopy (AFM). AFM is perf...

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Autores principales: Karkali, Katerina, Jorba, Ignasi, Navajas, Daniel, Martin-Blanco, Enrique
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9732408/
https://www.ncbi.nlm.nih.gov/pubmed/36595903
http://dx.doi.org/10.1016/j.xpro.2022.101901
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author Karkali, Katerina
Jorba, Ignasi
Navajas, Daniel
Martin-Blanco, Enrique
author_facet Karkali, Katerina
Jorba, Ignasi
Navajas, Daniel
Martin-Blanco, Enrique
author_sort Karkali, Katerina
collection PubMed
description Drosophila is an amenable system for addressing the mechanics of morphogenesis. We describe a workflow for characterizing the mechanical properties of its ventral nerve cord (VNC), at different developmental stages, in live, flat-dissected embryos employing atomic force microscopy (AFM). AFM is performed with spherical probes, and stiffness (Young’s modulus) is calculated by fitting force curves with Hertz’s contact model. For complete details on the use and execution of this protocol, please refer to Karkali et al. (2022).
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spelling pubmed-97324082022-12-10 Measuring ventral nerve cord stiffness in live flat-dissected Drosophila embryos by atomic force microscopy Karkali, Katerina Jorba, Ignasi Navajas, Daniel Martin-Blanco, Enrique STAR Protoc Protocol Drosophila is an amenable system for addressing the mechanics of morphogenesis. We describe a workflow for characterizing the mechanical properties of its ventral nerve cord (VNC), at different developmental stages, in live, flat-dissected embryos employing atomic force microscopy (AFM). AFM is performed with spherical probes, and stiffness (Young’s modulus) is calculated by fitting force curves with Hertz’s contact model. For complete details on the use and execution of this protocol, please refer to Karkali et al. (2022). Elsevier 2022-12-05 /pmc/articles/PMC9732408/ /pubmed/36595903 http://dx.doi.org/10.1016/j.xpro.2022.101901 Text en © 2022 The Authors https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Karkali, Katerina
Jorba, Ignasi
Navajas, Daniel
Martin-Blanco, Enrique
Measuring ventral nerve cord stiffness in live flat-dissected Drosophila embryos by atomic force microscopy
title Measuring ventral nerve cord stiffness in live flat-dissected Drosophila embryos by atomic force microscopy
title_full Measuring ventral nerve cord stiffness in live flat-dissected Drosophila embryos by atomic force microscopy
title_fullStr Measuring ventral nerve cord stiffness in live flat-dissected Drosophila embryos by atomic force microscopy
title_full_unstemmed Measuring ventral nerve cord stiffness in live flat-dissected Drosophila embryos by atomic force microscopy
title_short Measuring ventral nerve cord stiffness in live flat-dissected Drosophila embryos by atomic force microscopy
title_sort measuring ventral nerve cord stiffness in live flat-dissected drosophila embryos by atomic force microscopy
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9732408/
https://www.ncbi.nlm.nih.gov/pubmed/36595903
http://dx.doi.org/10.1016/j.xpro.2022.101901
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