Cargando…

Automatic identifying and counting blood cells in smear images by using single shot detector and Taguchi method

BACKGROUND: Researchers have tried to identify and count different blood cells in microscopic smear images by using deep learning methods of artificial intelligence to solve the highly time-consuming problem. RESULTS: The three types of blood cells are platelets, red blood cells, and white blood cel...

Descripción completa

Detalles Bibliográficos
Autores principales: Chen, Yao-Mei, Tsai, Jinn-Tsong, Ho, Wen-Hsien
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9732976/
https://www.ncbi.nlm.nih.gov/pubmed/36482316
http://dx.doi.org/10.1186/s12859-022-05074-2
_version_ 1784846251373625344
author Chen, Yao-Mei
Tsai, Jinn-Tsong
Ho, Wen-Hsien
author_facet Chen, Yao-Mei
Tsai, Jinn-Tsong
Ho, Wen-Hsien
author_sort Chen, Yao-Mei
collection PubMed
description BACKGROUND: Researchers have tried to identify and count different blood cells in microscopic smear images by using deep learning methods of artificial intelligence to solve the highly time-consuming problem. RESULTS: The three types of blood cells are platelets, red blood cells, and white blood cells. This study used the Resnet50 network as a backbone network of the single shot detector (SSD) for automatically identifying and counting different blood cells and, meanwhile, proposed a systematic method to find a better combination of algorithm hyperparameters of the Resnet50 network for promoting accuracy for identifying and counting blood cells. The Resnet50 backbone network of the SSD with its optimized algorithm hyperparameters, which is called the Resnet50-SSD model, was developed to enhance the feature extraction ability for identifying and counting blood cells. Furthermore, the algorithm hyperparameters of Resnet50 backbone networks of the SSD were optimized by the Taguchi experimental method for promoting detection accuracy of the Resnet50-SSD model. The experimental result shows that the detection accuracy of the Resnet50-SSD model with 512 × 512 × 3 input images was better than that of the Resnet50-SSD model with 300 × 300 × 3 input images on the test set of blood cells images. Additionally, the detection accuracy of the Resnet50-SSD model using the combination of algorithm hyperparameters got by the Taguchi method was better than that of the Resnet50-SSD model using the combination of algorithm hyperparameters given by the Matlab example. CONCLUSION: In blood cell images acquired from the BCCD dataset, the proposed Resnet50-SSD model had higher accuracy in identifying and counting blood cells, especially white blood cells and red blood cells.
format Online
Article
Text
id pubmed-9732976
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher BioMed Central
record_format MEDLINE/PubMed
spelling pubmed-97329762022-12-10 Automatic identifying and counting blood cells in smear images by using single shot detector and Taguchi method Chen, Yao-Mei Tsai, Jinn-Tsong Ho, Wen-Hsien BMC Bioinformatics Research BACKGROUND: Researchers have tried to identify and count different blood cells in microscopic smear images by using deep learning methods of artificial intelligence to solve the highly time-consuming problem. RESULTS: The three types of blood cells are platelets, red blood cells, and white blood cells. This study used the Resnet50 network as a backbone network of the single shot detector (SSD) for automatically identifying and counting different blood cells and, meanwhile, proposed a systematic method to find a better combination of algorithm hyperparameters of the Resnet50 network for promoting accuracy for identifying and counting blood cells. The Resnet50 backbone network of the SSD with its optimized algorithm hyperparameters, which is called the Resnet50-SSD model, was developed to enhance the feature extraction ability for identifying and counting blood cells. Furthermore, the algorithm hyperparameters of Resnet50 backbone networks of the SSD were optimized by the Taguchi experimental method for promoting detection accuracy of the Resnet50-SSD model. The experimental result shows that the detection accuracy of the Resnet50-SSD model with 512 × 512 × 3 input images was better than that of the Resnet50-SSD model with 300 × 300 × 3 input images on the test set of blood cells images. Additionally, the detection accuracy of the Resnet50-SSD model using the combination of algorithm hyperparameters got by the Taguchi method was better than that of the Resnet50-SSD model using the combination of algorithm hyperparameters given by the Matlab example. CONCLUSION: In blood cell images acquired from the BCCD dataset, the proposed Resnet50-SSD model had higher accuracy in identifying and counting blood cells, especially white blood cells and red blood cells. BioMed Central 2022-12-08 /pmc/articles/PMC9732976/ /pubmed/36482316 http://dx.doi.org/10.1186/s12859-022-05074-2 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research
Chen, Yao-Mei
Tsai, Jinn-Tsong
Ho, Wen-Hsien
Automatic identifying and counting blood cells in smear images by using single shot detector and Taguchi method
title Automatic identifying and counting blood cells in smear images by using single shot detector and Taguchi method
title_full Automatic identifying and counting blood cells in smear images by using single shot detector and Taguchi method
title_fullStr Automatic identifying and counting blood cells in smear images by using single shot detector and Taguchi method
title_full_unstemmed Automatic identifying and counting blood cells in smear images by using single shot detector and Taguchi method
title_short Automatic identifying and counting blood cells in smear images by using single shot detector and Taguchi method
title_sort automatic identifying and counting blood cells in smear images by using single shot detector and taguchi method
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9732976/
https://www.ncbi.nlm.nih.gov/pubmed/36482316
http://dx.doi.org/10.1186/s12859-022-05074-2
work_keys_str_mv AT chenyaomei automaticidentifyingandcountingbloodcellsinsmearimagesbyusingsingleshotdetectorandtaguchimethod
AT tsaijinntsong automaticidentifyingandcountingbloodcellsinsmearimagesbyusingsingleshotdetectorandtaguchimethod
AT howenhsien automaticidentifyingandcountingbloodcellsinsmearimagesbyusingsingleshotdetectorandtaguchimethod