Evaluation of novel surfactants for plant transformation

OBJECTIVE: Assess the efficiency of seven novel surfactants, relative to the typical Silwet(®) L-77, for floral dip transformation of Arabidopsis. RESULTS: Floral dip transformation of Arabidopsis has been used consistently for 20 years with little change in the protocol. Here we directly compare se...

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Detalles Bibliográficos
Autores principales: Huynh, Jennie, Hotton, Sara K., Chan, Ron, Syed, Yasra, Thomson, James
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2022
Materias:
Research Note
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9733262/
https://www.ncbi.nlm.nih.gov/pubmed/36482477
http://dx.doi.org/10.1186/s13104-022-06251-5
Descripción
Sumario:OBJECTIVE: Assess the efficiency of seven novel surfactants, relative to the typical Silwet(®) L-77, for floral dip transformation of Arabidopsis. RESULTS: Floral dip transformation of Arabidopsis has been used consistently for 20 years with little change in the protocol. Here we directly compare seven novel surfactants (BREAK-THRU(®)-OE446, S200, S233, S240, S279, S301 and SP133) to the standard Silwet(®) L-77 for efficiency of Arabidopsis transformation providing an example of how the surfactants can help other plant transformation protocols. Relative transformation efficiencies ranged from − 44 to + 45% compared to Silwet(®) L-77. Surfactants S200, S240, and S279 demonstrated the greatest enhancement in transformation. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s13104-022-06251-5.

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