Evaluation of novel surfactants for plant transformation

OBJECTIVE: Assess the efficiency of seven novel surfactants, relative to the typical Silwet(®) L-77, for floral dip transformation of Arabidopsis. RESULTS: Floral dip transformation of Arabidopsis has been used consistently for 20 years with little change in the protocol. Here we directly compare se...

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Autores principales: Huynh, Jennie, Hotton, Sara K., Chan, Ron, Syed, Yasra, Thomson, James
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2022
Materias:
Research Note
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9733262/
https://www.ncbi.nlm.nih.gov/pubmed/36482477
http://dx.doi.org/10.1186/s13104-022-06251-5
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author Huynh, Jennie
Hotton, Sara K.
Chan, Ron
Syed, Yasra
Thomson, James
author_facet Huynh, Jennie
Hotton, Sara K.
Chan, Ron
Syed, Yasra
Thomson, James
author_sort Huynh, Jennie
collection PubMed
description OBJECTIVE: Assess the efficiency of seven novel surfactants, relative to the typical Silwet(®) L-77, for floral dip transformation of Arabidopsis. RESULTS: Floral dip transformation of Arabidopsis has been used consistently for 20 years with little change in the protocol. Here we directly compare seven novel surfactants (BREAK-THRU(®)-OE446, S200, S233, S240, S279, S301 and SP133) to the standard Silwet(®) L-77 for efficiency of Arabidopsis transformation providing an example of how the surfactants can help other plant transformation protocols. Relative transformation efficiencies ranged from − 44 to + 45% compared to Silwet(®) L-77. Surfactants S200, S240, and S279 demonstrated the greatest enhancement in transformation. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s13104-022-06251-5.
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spelling pubmed-97332622022-12-10 Evaluation of novel surfactants for plant transformation Huynh, Jennie Hotton, Sara K. Chan, Ron Syed, Yasra Thomson, James BMC Res Notes Research Note OBJECTIVE: Assess the efficiency of seven novel surfactants, relative to the typical Silwet(®) L-77, for floral dip transformation of Arabidopsis. RESULTS: Floral dip transformation of Arabidopsis has been used consistently for 20 years with little change in the protocol. Here we directly compare seven novel surfactants (BREAK-THRU(®)-OE446, S200, S233, S240, S279, S301 and SP133) to the standard Silwet(®) L-77 for efficiency of Arabidopsis transformation providing an example of how the surfactants can help other plant transformation protocols. Relative transformation efficiencies ranged from − 44 to + 45% compared to Silwet(®) L-77. Surfactants S200, S240, and S279 demonstrated the greatest enhancement in transformation. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s13104-022-06251-5. BioMed Central 2022-12-08 /pmc/articles/PMC9733262/ /pubmed/36482477 http://dx.doi.org/10.1186/s13104-022-06251-5 Text en © This is a U.S. Government work and not under copyright protection in the US; foreign copyright protection may apply 2022 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research Note
Huynh, Jennie
Hotton, Sara K.
Chan, Ron
Syed, Yasra
Thomson, James
Evaluation of novel surfactants for plant transformation
title Evaluation of novel surfactants for plant transformation
title_full Evaluation of novel surfactants for plant transformation
title_fullStr Evaluation of novel surfactants for plant transformation
title_full_unstemmed Evaluation of novel surfactants for plant transformation
title_short Evaluation of novel surfactants for plant transformation
title_sort evaluation of novel surfactants for plant transformation
topic Research Note
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9733262/
https://www.ncbi.nlm.nih.gov/pubmed/36482477
http://dx.doi.org/10.1186/s13104-022-06251-5
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