Biological isolation and characterization of Catharanthus roseus (L.) G. Don methanolic leaves extracts and their assessment for antimicrobial, cytotoxic, and apoptotic activities

BACKGROUND: Biological synthesis of natural products from plants has made us an inspiring methodology in the field of science and biotechnology. METHODS: The methanolic extracts of Catharanthus roseus (L.) G. Don plant leaves (CrPLE) were extracted and characterized by utilizing the phytochemicals e...

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Autores principales: Rajashekara, Somashekara, Reena, Dondapati, Mainavi, Mullahalli Venkataramareddy, Sandhya, Locheruvapalli Srinivasa, Baro, Utpal
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2022
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9733275/
https://www.ncbi.nlm.nih.gov/pubmed/36494692
http://dx.doi.org/10.1186/s12906-022-03810-y
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author Rajashekara, Somashekara
Reena, Dondapati
Mainavi, Mullahalli Venkataramareddy
Sandhya, Locheruvapalli Srinivasa
Baro, Utpal
author_facet Rajashekara, Somashekara
Reena, Dondapati
Mainavi, Mullahalli Venkataramareddy
Sandhya, Locheruvapalli Srinivasa
Baro, Utpal
author_sort Rajashekara, Somashekara
collection PubMed
description BACKGROUND: Biological synthesis of natural products from plants has made us an inspiring methodology in the field of science and biotechnology. METHODS: The methanolic extracts of Catharanthus roseus (L.) G. Don plant leaves (CrPLE) were extracted and characterized by utilizing the phytochemicals estimation, Thin-layer chromatography (TLC), and High-Performance Liquid Chromatography (HPLC) analysis; and further evaluation for an understanding of the biomedical uses of CrPLE was done. RESULTS: The evaluation of the seven phytochemicals designates the presence of secondary metabolites in the CrPLE. The CrPLE (test samples) exhibited the Catechin and Caffeic acid contents of 0.0055 and 0.0149 mg/g respectively. The CrPLE revealed the highest antimicrobial activity and showed a mortal effect against the tested microorganisms. Cytotoxicity of the breast cancer cell lines was exposed that CrPLE as a respectable anticancer specialist and metabolically vigorous cells. CONCLUSION: Consequently, the biological synthesized methanolic leaf extracts of the C. roseus plants would be appreciated and have incredible contributions to the field of medicinal applications. GRAPHICAL ABSTRACT: [Image: see text]
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spelling pubmed-97332752022-12-10 Biological isolation and characterization of Catharanthus roseus (L.) G. Don methanolic leaves extracts and their assessment for antimicrobial, cytotoxic, and apoptotic activities Rajashekara, Somashekara Reena, Dondapati Mainavi, Mullahalli Venkataramareddy Sandhya, Locheruvapalli Srinivasa Baro, Utpal BMC Complement Med Ther Research Article BACKGROUND: Biological synthesis of natural products from plants has made us an inspiring methodology in the field of science and biotechnology. METHODS: The methanolic extracts of Catharanthus roseus (L.) G. Don plant leaves (CrPLE) were extracted and characterized by utilizing the phytochemicals estimation, Thin-layer chromatography (TLC), and High-Performance Liquid Chromatography (HPLC) analysis; and further evaluation for an understanding of the biomedical uses of CrPLE was done. RESULTS: The evaluation of the seven phytochemicals designates the presence of secondary metabolites in the CrPLE. The CrPLE (test samples) exhibited the Catechin and Caffeic acid contents of 0.0055 and 0.0149 mg/g respectively. The CrPLE revealed the highest antimicrobial activity and showed a mortal effect against the tested microorganisms. Cytotoxicity of the breast cancer cell lines was exposed that CrPLE as a respectable anticancer specialist and metabolically vigorous cells. CONCLUSION: Consequently, the biological synthesized methanolic leaf extracts of the C. roseus plants would be appreciated and have incredible contributions to the field of medicinal applications. GRAPHICAL ABSTRACT: [Image: see text] BioMed Central 2022-12-09 /pmc/articles/PMC9733275/ /pubmed/36494692 http://dx.doi.org/10.1186/s12906-022-03810-y Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research Article
Rajashekara, Somashekara
Reena, Dondapati
Mainavi, Mullahalli Venkataramareddy
Sandhya, Locheruvapalli Srinivasa
Baro, Utpal
Biological isolation and characterization of Catharanthus roseus (L.) G. Don methanolic leaves extracts and their assessment for antimicrobial, cytotoxic, and apoptotic activities
title Biological isolation and characterization of Catharanthus roseus (L.) G. Don methanolic leaves extracts and their assessment for antimicrobial, cytotoxic, and apoptotic activities
title_full Biological isolation and characterization of Catharanthus roseus (L.) G. Don methanolic leaves extracts and their assessment for antimicrobial, cytotoxic, and apoptotic activities
title_fullStr Biological isolation and characterization of Catharanthus roseus (L.) G. Don methanolic leaves extracts and their assessment for antimicrobial, cytotoxic, and apoptotic activities
title_full_unstemmed Biological isolation and characterization of Catharanthus roseus (L.) G. Don methanolic leaves extracts and their assessment for antimicrobial, cytotoxic, and apoptotic activities
title_short Biological isolation and characterization of Catharanthus roseus (L.) G. Don methanolic leaves extracts and their assessment for antimicrobial, cytotoxic, and apoptotic activities
title_sort biological isolation and characterization of catharanthus roseus (l.) g. don methanolic leaves extracts and their assessment for antimicrobial, cytotoxic, and apoptotic activities
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9733275/
https://www.ncbi.nlm.nih.gov/pubmed/36494692
http://dx.doi.org/10.1186/s12906-022-03810-y
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