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A new in vitro monitoring system reveals a specific influence of Arabidopsis nitrogen nutrition on its susceptibility to Alternaria brassicicola at the seedling stage
BACKGROUND: Seedling growth is an early phase of plant development highly susceptible to environmental factors such as soil nitrogen (N) availability or presence of seed-borne pathogens. Whereas N plays a central role in plant-pathogen interactions, its role has never been studied during this early...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9733346/ https://www.ncbi.nlm.nih.gov/pubmed/36482365 http://dx.doi.org/10.1186/s13007-022-00962-3 |
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author | Barrit, Thibault Campion, Claire Aligon, Sophie Bourbeillon, Julie Rousseau, David Planchet, Elisabeth Teulat, Béatrice |
author_facet | Barrit, Thibault Campion, Claire Aligon, Sophie Bourbeillon, Julie Rousseau, David Planchet, Elisabeth Teulat, Béatrice |
author_sort | Barrit, Thibault |
collection | PubMed |
description | BACKGROUND: Seedling growth is an early phase of plant development highly susceptible to environmental factors such as soil nitrogen (N) availability or presence of seed-borne pathogens. Whereas N plays a central role in plant-pathogen interactions, its role has never been studied during this early phase for the interaction between Arabidopsis thaliana and Alternaria brassicicola, a seed-transmitted necrotrophic fungus. The aim of the present work was to develop an in vitro monitoring system allowing to study the impact of the fungus on A. thaliana seedling growth, while modulating N nutrition. RESULTS: The developed system consists of square plates placed vertically and filled with nutrient agar medium allowing modulation of N conditions. Seeds are inoculated after sowing by depositing a droplet of conidial suspension. A specific semi-automated image analysis pipeline based on the Ilastik software was developed to quantify the impact of the fungus on seedling aerial development, calculating an index accounting for every aspect of fungal impact, namely seedling death, necrosis and developmental delay. The system also permits to monitor root elongation. The interest of the system was then confirmed by characterising how N media composition [0.1 and 5 mM of nitrate (NO(3)(−)), 5 mM of ammonium (NH(4)(+))] affects the impact of the fungus on three A. thaliana ecotypes. Seedling development was strongly and negatively affected by the fungus. However, seedlings grown with 5 mM NO(3)(−) were less susceptible than those grown with NH(4)(+) or 0.1 mM NO(3)(−), which differed from what was observed with adult plants (rosette stage). CONCLUSIONS: The developed monitoring system allows accurate determination of seedling growth characteristics (both on aerial and root parts) and symptoms. Altogether, this system could be used to study the impact of plant nutrition on susceptibility of various genotypes to fungi at the seedling stage. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s13007-022-00962-3. |
format | Online Article Text |
id | pubmed-9733346 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-97333462022-12-10 A new in vitro monitoring system reveals a specific influence of Arabidopsis nitrogen nutrition on its susceptibility to Alternaria brassicicola at the seedling stage Barrit, Thibault Campion, Claire Aligon, Sophie Bourbeillon, Julie Rousseau, David Planchet, Elisabeth Teulat, Béatrice Plant Methods Research BACKGROUND: Seedling growth is an early phase of plant development highly susceptible to environmental factors such as soil nitrogen (N) availability or presence of seed-borne pathogens. Whereas N plays a central role in plant-pathogen interactions, its role has never been studied during this early phase for the interaction between Arabidopsis thaliana and Alternaria brassicicola, a seed-transmitted necrotrophic fungus. The aim of the present work was to develop an in vitro monitoring system allowing to study the impact of the fungus on A. thaliana seedling growth, while modulating N nutrition. RESULTS: The developed system consists of square plates placed vertically and filled with nutrient agar medium allowing modulation of N conditions. Seeds are inoculated after sowing by depositing a droplet of conidial suspension. A specific semi-automated image analysis pipeline based on the Ilastik software was developed to quantify the impact of the fungus on seedling aerial development, calculating an index accounting for every aspect of fungal impact, namely seedling death, necrosis and developmental delay. The system also permits to monitor root elongation. The interest of the system was then confirmed by characterising how N media composition [0.1 and 5 mM of nitrate (NO(3)(−)), 5 mM of ammonium (NH(4)(+))] affects the impact of the fungus on three A. thaliana ecotypes. Seedling development was strongly and negatively affected by the fungus. However, seedlings grown with 5 mM NO(3)(−) were less susceptible than those grown with NH(4)(+) or 0.1 mM NO(3)(−), which differed from what was observed with adult plants (rosette stage). CONCLUSIONS: The developed monitoring system allows accurate determination of seedling growth characteristics (both on aerial and root parts) and symptoms. Altogether, this system could be used to study the impact of plant nutrition on susceptibility of various genotypes to fungi at the seedling stage. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s13007-022-00962-3. BioMed Central 2022-12-08 /pmc/articles/PMC9733346/ /pubmed/36482365 http://dx.doi.org/10.1186/s13007-022-00962-3 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data. |
spellingShingle | Research Barrit, Thibault Campion, Claire Aligon, Sophie Bourbeillon, Julie Rousseau, David Planchet, Elisabeth Teulat, Béatrice A new in vitro monitoring system reveals a specific influence of Arabidopsis nitrogen nutrition on its susceptibility to Alternaria brassicicola at the seedling stage |
title | A new in vitro monitoring system reveals a specific influence of Arabidopsis nitrogen nutrition on its susceptibility to Alternaria brassicicola at the seedling stage |
title_full | A new in vitro monitoring system reveals a specific influence of Arabidopsis nitrogen nutrition on its susceptibility to Alternaria brassicicola at the seedling stage |
title_fullStr | A new in vitro monitoring system reveals a specific influence of Arabidopsis nitrogen nutrition on its susceptibility to Alternaria brassicicola at the seedling stage |
title_full_unstemmed | A new in vitro monitoring system reveals a specific influence of Arabidopsis nitrogen nutrition on its susceptibility to Alternaria brassicicola at the seedling stage |
title_short | A new in vitro monitoring system reveals a specific influence of Arabidopsis nitrogen nutrition on its susceptibility to Alternaria brassicicola at the seedling stage |
title_sort | new in vitro monitoring system reveals a specific influence of arabidopsis nitrogen nutrition on its susceptibility to alternaria brassicicola at the seedling stage |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9733346/ https://www.ncbi.nlm.nih.gov/pubmed/36482365 http://dx.doi.org/10.1186/s13007-022-00962-3 |
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