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Differential transcriptomic changes in the central nervous system and urinary bladders of mice infected with a coronavirus
Multiple sclerosis (MS) often leads to the development of neurogenic lower urinary tract symptoms (LUTS). We previously characterized neurogenic bladder dysfunction in a mouse model of MS induced by a coronavirus, mouse hepatitis virus (MHV). The aim of the study was to identify genes and pathways l...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Public Library of Science
2022
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9733897/ https://www.ncbi.nlm.nih.gov/pubmed/36490282 http://dx.doi.org/10.1371/journal.pone.0278918 |
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author | Clarkson, Taylor C. Iguchi, Nao Xie, Alison Xiaoqiao Malykhina, Anna P. |
author_facet | Clarkson, Taylor C. Iguchi, Nao Xie, Alison Xiaoqiao Malykhina, Anna P. |
author_sort | Clarkson, Taylor C. |
collection | PubMed |
description | Multiple sclerosis (MS) often leads to the development of neurogenic lower urinary tract symptoms (LUTS). We previously characterized neurogenic bladder dysfunction in a mouse model of MS induced by a coronavirus, mouse hepatitis virus (MHV). The aim of the study was to identify genes and pathways linking neuroinflammation in the central nervous system with urinary bladder (UB) dysfunction to enhance our understanding of the mechanisms underlying LUTS in demyelinating diseases. Adult C57BL/6 male mice (N = 12) received either an intracranial injection of MHV (coronavirus-induced encephalomyelitis, CIE group), or sterile saline (control group). Spinal cord (SC) and urinary bladders (UB) were collected from CIE mice at 1 wk and 4 wks, followed by RNA isolation and NanoString nCounter Neuroinflammation assay. Transcriptome analysis of SC identified a significantly changed expression of >150 genes in CIE mice known to regulate astrocyte, microglia and oligodendrocyte functions, neuroinflammation and immune responses. Two genes were significantly upregulated (Ttr and Ms4a4a), and two were downregulated (Asb2 and Myct1) only in the UB of CIE mice. Siglec1 and Zbp1 were the only genes significantly upregulated in both tissues, suggesting a common transcriptomic link between neuroinflammation in the CNS and neurogenic changes in the UB of CIE mice. |
format | Online Article Text |
id | pubmed-9733897 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-97338972022-12-10 Differential transcriptomic changes in the central nervous system and urinary bladders of mice infected with a coronavirus Clarkson, Taylor C. Iguchi, Nao Xie, Alison Xiaoqiao Malykhina, Anna P. PLoS One Research Article Multiple sclerosis (MS) often leads to the development of neurogenic lower urinary tract symptoms (LUTS). We previously characterized neurogenic bladder dysfunction in a mouse model of MS induced by a coronavirus, mouse hepatitis virus (MHV). The aim of the study was to identify genes and pathways linking neuroinflammation in the central nervous system with urinary bladder (UB) dysfunction to enhance our understanding of the mechanisms underlying LUTS in demyelinating diseases. Adult C57BL/6 male mice (N = 12) received either an intracranial injection of MHV (coronavirus-induced encephalomyelitis, CIE group), or sterile saline (control group). Spinal cord (SC) and urinary bladders (UB) were collected from CIE mice at 1 wk and 4 wks, followed by RNA isolation and NanoString nCounter Neuroinflammation assay. Transcriptome analysis of SC identified a significantly changed expression of >150 genes in CIE mice known to regulate astrocyte, microglia and oligodendrocyte functions, neuroinflammation and immune responses. Two genes were significantly upregulated (Ttr and Ms4a4a), and two were downregulated (Asb2 and Myct1) only in the UB of CIE mice. Siglec1 and Zbp1 were the only genes significantly upregulated in both tissues, suggesting a common transcriptomic link between neuroinflammation in the CNS and neurogenic changes in the UB of CIE mice. Public Library of Science 2022-12-09 /pmc/articles/PMC9733897/ /pubmed/36490282 http://dx.doi.org/10.1371/journal.pone.0278918 Text en © 2022 Clarkson et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Clarkson, Taylor C. Iguchi, Nao Xie, Alison Xiaoqiao Malykhina, Anna P. Differential transcriptomic changes in the central nervous system and urinary bladders of mice infected with a coronavirus |
title | Differential transcriptomic changes in the central nervous system and urinary bladders of mice infected with a coronavirus |
title_full | Differential transcriptomic changes in the central nervous system and urinary bladders of mice infected with a coronavirus |
title_fullStr | Differential transcriptomic changes in the central nervous system and urinary bladders of mice infected with a coronavirus |
title_full_unstemmed | Differential transcriptomic changes in the central nervous system and urinary bladders of mice infected with a coronavirus |
title_short | Differential transcriptomic changes in the central nervous system and urinary bladders of mice infected with a coronavirus |
title_sort | differential transcriptomic changes in the central nervous system and urinary bladders of mice infected with a coronavirus |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9733897/ https://www.ncbi.nlm.nih.gov/pubmed/36490282 http://dx.doi.org/10.1371/journal.pone.0278918 |
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