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In vitro modelling of human proprioceptive sensory neurons in the neuromuscular system
Proprioceptive sensory neurons (pSN) are an essential and undervalued part of the neuromuscular circuit. A protocol to differentiate healthy and amyotrophic lateral sclerosis (ALS) human neural stem cells (hNSC) into pSN, and their comparison with the motor neuron (MN) differentiation process from t...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9734133/ https://www.ncbi.nlm.nih.gov/pubmed/36494423 http://dx.doi.org/10.1038/s41598-022-23565-3 |
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author | Badiola-Mateos, Maider Osaki, Tatsuya Kamm, Roger Dale Samitier, Josep |
author_facet | Badiola-Mateos, Maider Osaki, Tatsuya Kamm, Roger Dale Samitier, Josep |
author_sort | Badiola-Mateos, Maider |
collection | PubMed |
description | Proprioceptive sensory neurons (pSN) are an essential and undervalued part of the neuromuscular circuit. A protocol to differentiate healthy and amyotrophic lateral sclerosis (ALS) human neural stem cells (hNSC) into pSN, and their comparison with the motor neuron (MN) differentiation process from the same hNSC sources, facilitated the development of in vitro co-culture platforms. The obtained pSN spheroids cultured interact with human skeletal myocytes showing the formation of annulospiral wrapping-like structures between TrkC + neurons and a multinucleated muscle fibre, presenting synaptic bouton-like structures in the contact point. The comparative analysis of the genetic profile performed in healthy and sporadic ALS hNSC differentiated to pSN suggested that basal levels of ETV1, critical for motor feedback from pSN, were much lower for ALS samples and that the differences between healthy and ALS samples, suggest the involvement of pSN in ALS pathology development and progression. |
format | Online Article Text |
id | pubmed-9734133 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-97341332022-12-11 In vitro modelling of human proprioceptive sensory neurons in the neuromuscular system Badiola-Mateos, Maider Osaki, Tatsuya Kamm, Roger Dale Samitier, Josep Sci Rep Article Proprioceptive sensory neurons (pSN) are an essential and undervalued part of the neuromuscular circuit. A protocol to differentiate healthy and amyotrophic lateral sclerosis (ALS) human neural stem cells (hNSC) into pSN, and their comparison with the motor neuron (MN) differentiation process from the same hNSC sources, facilitated the development of in vitro co-culture platforms. The obtained pSN spheroids cultured interact with human skeletal myocytes showing the formation of annulospiral wrapping-like structures between TrkC + neurons and a multinucleated muscle fibre, presenting synaptic bouton-like structures in the contact point. The comparative analysis of the genetic profile performed in healthy and sporadic ALS hNSC differentiated to pSN suggested that basal levels of ETV1, critical for motor feedback from pSN, were much lower for ALS samples and that the differences between healthy and ALS samples, suggest the involvement of pSN in ALS pathology development and progression. Nature Publishing Group UK 2022-12-09 /pmc/articles/PMC9734133/ /pubmed/36494423 http://dx.doi.org/10.1038/s41598-022-23565-3 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Article Badiola-Mateos, Maider Osaki, Tatsuya Kamm, Roger Dale Samitier, Josep In vitro modelling of human proprioceptive sensory neurons in the neuromuscular system |
title | In vitro modelling of human proprioceptive sensory neurons in the neuromuscular system |
title_full | In vitro modelling of human proprioceptive sensory neurons in the neuromuscular system |
title_fullStr | In vitro modelling of human proprioceptive sensory neurons in the neuromuscular system |
title_full_unstemmed | In vitro modelling of human proprioceptive sensory neurons in the neuromuscular system |
title_short | In vitro modelling of human proprioceptive sensory neurons in the neuromuscular system |
title_sort | in vitro modelling of human proprioceptive sensory neurons in the neuromuscular system |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9734133/ https://www.ncbi.nlm.nih.gov/pubmed/36494423 http://dx.doi.org/10.1038/s41598-022-23565-3 |
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