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Emergency vaccination of cattle against lumpy skin disease: Evaluation of safety, efficacy, and potency of MEVAC(®) LSD vaccine containing Neethling strain
Lumpy skin disease (LSD) is an emerging disease of cattle causing significantly high economic losses. Control of LSD depends on the use of homologous attenuated LSD virus strains isolated originally from South Africa (the Neethling strain). The virus belongs to the genus Capripoxvirus, which include...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer Netherlands
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9734455/ https://www.ncbi.nlm.nih.gov/pubmed/36460903 http://dx.doi.org/10.1007/s11259-022-10037-2 |
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author | Bazid, Abdel-Hamid Wasfy, Momtaz Fawzy, Mohamed Nayel, Mohamed Abdelmegeid, Mohamed Thabet, Randa Y. Yong, Hui Sian El-Sayed, Magdy M. Magouz, Asmaa Badr, Yassien |
author_facet | Bazid, Abdel-Hamid Wasfy, Momtaz Fawzy, Mohamed Nayel, Mohamed Abdelmegeid, Mohamed Thabet, Randa Y. Yong, Hui Sian El-Sayed, Magdy M. Magouz, Asmaa Badr, Yassien |
author_sort | Bazid, Abdel-Hamid |
collection | PubMed |
description | Lumpy skin disease (LSD) is an emerging disease of cattle causing significantly high economic losses. Control of LSD depends on the use of homologous attenuated LSD virus strains isolated originally from South Africa (the Neethling strain). The virus belongs to the genus Capripoxvirus, which includes sheep pox virus and goat pox virus. The present study was conducted to evaluate the safety and efficacy of a new live attenuated LSD vaccine produced by Middle East for Vaccines (MEVAC(®)) based on the Neethling strain. Tests were performed both in Egypt and Vietnam. Safety was evaluated by inoculation of five cattle with 10 times the recommended dose and observation of the animals for 14 days. Immunogenicity was tested at different periods post-vaccination (PV) in animals receiving the recommended doses of the vaccine using ELISA and virus neutralization test. Five cows were used to determine the protection index (PI) and non-vaccinated control cattle were included. Three calves were challenged by intradermal inoculation of the wild virus (5 × 10(5) TCID(50)) 28 days PV. Field or mass vaccination experiments were conducted in Vietnam during national campaigns in the summer of 2021 with 4301 vaccinated animals closely monitored after vaccination. In the field, around 2% (80/4301) of the animals showed hyper-reactivity, and 0.6% (24/4301) showed small skin swellings that disappeared within few hours PV. Abortion was recorded in three animals (0.3% 3/867). Challenged animals were resistant to clinical disease and PI value was 3.5 log(10). Meanwhile, antibody levels determined by the ELISA were inconsistent among animals and laboratories during the study period. Overall, the findings point to a new safe and effective LSD vaccine. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s11259-022-10037-2. |
format | Online Article Text |
id | pubmed-9734455 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Springer Netherlands |
record_format | MEDLINE/PubMed |
spelling | pubmed-97344552022-12-12 Emergency vaccination of cattle against lumpy skin disease: Evaluation of safety, efficacy, and potency of MEVAC(®) LSD vaccine containing Neethling strain Bazid, Abdel-Hamid Wasfy, Momtaz Fawzy, Mohamed Nayel, Mohamed Abdelmegeid, Mohamed Thabet, Randa Y. Yong, Hui Sian El-Sayed, Magdy M. Magouz, Asmaa Badr, Yassien Vet Res Commun Research Lumpy skin disease (LSD) is an emerging disease of cattle causing significantly high economic losses. Control of LSD depends on the use of homologous attenuated LSD virus strains isolated originally from South Africa (the Neethling strain). The virus belongs to the genus Capripoxvirus, which includes sheep pox virus and goat pox virus. The present study was conducted to evaluate the safety and efficacy of a new live attenuated LSD vaccine produced by Middle East for Vaccines (MEVAC(®)) based on the Neethling strain. Tests were performed both in Egypt and Vietnam. Safety was evaluated by inoculation of five cattle with 10 times the recommended dose and observation of the animals for 14 days. Immunogenicity was tested at different periods post-vaccination (PV) in animals receiving the recommended doses of the vaccine using ELISA and virus neutralization test. Five cows were used to determine the protection index (PI) and non-vaccinated control cattle were included. Three calves were challenged by intradermal inoculation of the wild virus (5 × 10(5) TCID(50)) 28 days PV. Field or mass vaccination experiments were conducted in Vietnam during national campaigns in the summer of 2021 with 4301 vaccinated animals closely monitored after vaccination. In the field, around 2% (80/4301) of the animals showed hyper-reactivity, and 0.6% (24/4301) showed small skin swellings that disappeared within few hours PV. Abortion was recorded in three animals (0.3% 3/867). Challenged animals were resistant to clinical disease and PI value was 3.5 log(10). Meanwhile, antibody levels determined by the ELISA were inconsistent among animals and laboratories during the study period. Overall, the findings point to a new safe and effective LSD vaccine. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s11259-022-10037-2. Springer Netherlands 2022-12-03 2023 /pmc/articles/PMC9734455/ /pubmed/36460903 http://dx.doi.org/10.1007/s11259-022-10037-2 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Research Bazid, Abdel-Hamid Wasfy, Momtaz Fawzy, Mohamed Nayel, Mohamed Abdelmegeid, Mohamed Thabet, Randa Y. Yong, Hui Sian El-Sayed, Magdy M. Magouz, Asmaa Badr, Yassien Emergency vaccination of cattle against lumpy skin disease: Evaluation of safety, efficacy, and potency of MEVAC(®) LSD vaccine containing Neethling strain |
title | Emergency vaccination of cattle against lumpy skin disease: Evaluation of safety, efficacy, and potency of MEVAC(®) LSD vaccine containing Neethling strain |
title_full | Emergency vaccination of cattle against lumpy skin disease: Evaluation of safety, efficacy, and potency of MEVAC(®) LSD vaccine containing Neethling strain |
title_fullStr | Emergency vaccination of cattle against lumpy skin disease: Evaluation of safety, efficacy, and potency of MEVAC(®) LSD vaccine containing Neethling strain |
title_full_unstemmed | Emergency vaccination of cattle against lumpy skin disease: Evaluation of safety, efficacy, and potency of MEVAC(®) LSD vaccine containing Neethling strain |
title_short | Emergency vaccination of cattle against lumpy skin disease: Evaluation of safety, efficacy, and potency of MEVAC(®) LSD vaccine containing Neethling strain |
title_sort | emergency vaccination of cattle against lumpy skin disease: evaluation of safety, efficacy, and potency of mevac(®) lsd vaccine containing neethling strain |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9734455/ https://www.ncbi.nlm.nih.gov/pubmed/36460903 http://dx.doi.org/10.1007/s11259-022-10037-2 |
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