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Rapid Assessment of Neutralizing Antibodies Using Influenza Viruses with a Luciferase Reporter

Influenza viruses cause acute respiratory infections, especially in the autumn–winter period. They are characterized by a high mutation frequency and cause annual seasonal epidemics. The detection of antibodies that neutralize the virus is an important criterion in the assessment of population immun...

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Autores principales: Sergeeva, M. V., Pulkina, A. A., Romanovskaya-Romanko, E. A., Mustafaeva, A. S., Egorov, A. Yu., Stukova, M. A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Pleiades Publishing 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9734512/
https://www.ncbi.nlm.nih.gov/pubmed/36532244
http://dx.doi.org/10.1134/S0003683822070067
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author Sergeeva, M. V.
Pulkina, A. A.
Romanovskaya-Romanko, E. A.
Mustafaeva, A. S.
Egorov, A. Yu.
Stukova, M. A.
author_facet Sergeeva, M. V.
Pulkina, A. A.
Romanovskaya-Romanko, E. A.
Mustafaeva, A. S.
Egorov, A. Yu.
Stukova, M. A.
author_sort Sergeeva, M. V.
collection PubMed
description Influenza viruses cause acute respiratory infections, especially in the autumn–winter period. They are characterized by a high mutation frequency and cause annual seasonal epidemics. The detection of antibodies that neutralize the virus is an important criterion in the assessment of population immunity and the influenza vaccine effectiveness. In this study, a method for determining the titer of virus-neutralizing antibodies in blood serum has been developed. A new test called the luciferase neutralization assay uses a bioluminescent signal for detection. The assay is based on engineered influenza reporter viruses with various surface antigens and a nanoluciferase reporter protein in the NS1 reading frame. Using the developed method, we studied paired sera of volunteers obtained before and after vaccination. The proposed assay was compared with the conventional antibody assessment methods (microneutralization and hemagglutination inhibition assay); a high degree of correlation was observed. At the same time, the use of the luciferase neutralization assay made it possible to reduce the time required for the analysis and to simplify the detection procedure. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1134/S0003683822070067.
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spelling pubmed-97345122022-12-12 Rapid Assessment of Neutralizing Antibodies Using Influenza Viruses with a Luciferase Reporter Sergeeva, M. V. Pulkina, A. A. Romanovskaya-Romanko, E. A. Mustafaeva, A. S. Egorov, A. Yu. Stukova, M. A. Appl Biochem Microbiol Metrology, Standardization, and Control Influenza viruses cause acute respiratory infections, especially in the autumn–winter period. They are characterized by a high mutation frequency and cause annual seasonal epidemics. The detection of antibodies that neutralize the virus is an important criterion in the assessment of population immunity and the influenza vaccine effectiveness. In this study, a method for determining the titer of virus-neutralizing antibodies in blood serum has been developed. A new test called the luciferase neutralization assay uses a bioluminescent signal for detection. The assay is based on engineered influenza reporter viruses with various surface antigens and a nanoluciferase reporter protein in the NS1 reading frame. Using the developed method, we studied paired sera of volunteers obtained before and after vaccination. The proposed assay was compared with the conventional antibody assessment methods (microneutralization and hemagglutination inhibition assay); a high degree of correlation was observed. At the same time, the use of the luciferase neutralization assay made it possible to reduce the time required for the analysis and to simplify the detection procedure. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1134/S0003683822070067. Pleiades Publishing 2022-12-06 2022 /pmc/articles/PMC9734512/ /pubmed/36532244 http://dx.doi.org/10.1134/S0003683822070067 Text en © Pleiades Publishing, Inc. 2022, ISSN 0003-6838, Applied Biochemistry and Microbiology, 2022, Vol. 58, No. 7, pp. 878–886. © Pleiades Publishing, Inc., 2022.Russian Text © The Author(s), 2021, published in Biotekhnologiya, 2021, Vol. 37, No. 2, pp. 81–91. This article is made available via the PMC Open Access Subset for unrestricted research re-use and secondary analysis in any form or by any means with acknowledgement of the original source. These permissions are granted for the duration of the World Health Organization (WHO) declaration of COVID-19 as a global pandemic.
spellingShingle Metrology, Standardization, and Control
Sergeeva, M. V.
Pulkina, A. A.
Romanovskaya-Romanko, E. A.
Mustafaeva, A. S.
Egorov, A. Yu.
Stukova, M. A.
Rapid Assessment of Neutralizing Antibodies Using Influenza Viruses with a Luciferase Reporter
title Rapid Assessment of Neutralizing Antibodies Using Influenza Viruses with a Luciferase Reporter
title_full Rapid Assessment of Neutralizing Antibodies Using Influenza Viruses with a Luciferase Reporter
title_fullStr Rapid Assessment of Neutralizing Antibodies Using Influenza Viruses with a Luciferase Reporter
title_full_unstemmed Rapid Assessment of Neutralizing Antibodies Using Influenza Viruses with a Luciferase Reporter
title_short Rapid Assessment of Neutralizing Antibodies Using Influenza Viruses with a Luciferase Reporter
title_sort rapid assessment of neutralizing antibodies using influenza viruses with a luciferase reporter
topic Metrology, Standardization, and Control
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9734512/
https://www.ncbi.nlm.nih.gov/pubmed/36532244
http://dx.doi.org/10.1134/S0003683822070067
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