The anti-Alzheimer compounds from tempeh oil in LPS-induced neuronal Schwann cells

Tempeh is a traditional fermented Indonesian food from white soybean. Tempe has better nutritional value than non-fermented white soybean. The aim of this study was to extract tempeh oil and analyze the inhibitory potency of Alzheimer-related gene expression in LPS-induced neuronal Schwann cells. Te...

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Autores principales: Limanjaya, Eileen C., Subali, Dionysius, Yanti, Yanti
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2022
Materias:
Original Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9734740/
http://dx.doi.org/10.1186/s42779-022-00163-2
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author Limanjaya, Eileen C.
Subali, Dionysius
Yanti, Yanti
author_facet Limanjaya, Eileen C.
Subali, Dionysius
Yanti, Yanti
author_sort Limanjaya, Eileen C.
collection PubMed
description Tempeh is a traditional fermented Indonesian food from white soybean. Tempe has better nutritional value than non-fermented white soybean. The aim of this study was to extract tempeh oil and analyze the inhibitory potency of Alzheimer-related gene expression in LPS-induced neuronal Schwann cells. Tempeh oil was extracted with Bligh Dyer method and was analyzed with PUFA identification, anticholinesterase activity, antioxidant activity, and quantitative PCR. Tempeh oil had a total yield of 7.14%, and PUFA identification found 8.37% omega-3. The anti-acetylcholinesterase activity showed that tempeh oil 25 µg/mL had the highest activity and 500 µg/mL in anti-butyrylcholinesterase activity. The quantitative PCR showed that tempeh oil had downregulated the gene expression of PSEN1, Gsk3b, cdk5, and TNF. From this study, tempeh oil may have the potential to lower the risk of Alzheimer’s disease by regulating certain gene traits.
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spelling pubmed-97347402022-12-12 The anti-Alzheimer compounds from tempeh oil in LPS-induced neuronal Schwann cells Limanjaya, Eileen C. Subali, Dionysius Yanti, Yanti J. Ethn. Food Original Article Tempeh is a traditional fermented Indonesian food from white soybean. Tempe has better nutritional value than non-fermented white soybean. The aim of this study was to extract tempeh oil and analyze the inhibitory potency of Alzheimer-related gene expression in LPS-induced neuronal Schwann cells. Tempeh oil was extracted with Bligh Dyer method and was analyzed with PUFA identification, anticholinesterase activity, antioxidant activity, and quantitative PCR. Tempeh oil had a total yield of 7.14%, and PUFA identification found 8.37% omega-3. The anti-acetylcholinesterase activity showed that tempeh oil 25 µg/mL had the highest activity and 500 µg/mL in anti-butyrylcholinesterase activity. The quantitative PCR showed that tempeh oil had downregulated the gene expression of PSEN1, Gsk3b, cdk5, and TNF. From this study, tempeh oil may have the potential to lower the risk of Alzheimer’s disease by regulating certain gene traits. BioMed Central 2022-12-08 2022 /pmc/articles/PMC9734740/ http://dx.doi.org/10.1186/s42779-022-00163-2 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Original Article
Limanjaya, Eileen C.
Subali, Dionysius
Yanti, Yanti
The anti-Alzheimer compounds from tempeh oil in LPS-induced neuronal Schwann cells
title The anti-Alzheimer compounds from tempeh oil in LPS-induced neuronal Schwann cells
title_full The anti-Alzheimer compounds from tempeh oil in LPS-induced neuronal Schwann cells
title_fullStr The anti-Alzheimer compounds from tempeh oil in LPS-induced neuronal Schwann cells
title_full_unstemmed The anti-Alzheimer compounds from tempeh oil in LPS-induced neuronal Schwann cells
title_short The anti-Alzheimer compounds from tempeh oil in LPS-induced neuronal Schwann cells
title_sort anti-alzheimer compounds from tempeh oil in lps-induced neuronal schwann cells
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9734740/
http://dx.doi.org/10.1186/s42779-022-00163-2
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