Wastewater-based surveillance in Italy leading to the first detection of mcr-10-positive Klebsiella quasipneumoniae

Wastewater-based surveillance enabled the first detection of the mobile colistin resistance gene mcr-10 in Italy. This plasmid-borne resistance gene was found in strains of Klebsiella quasipneumoniae isolated from samples of human raw sewage collected over several months. Although the isolates were...

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Autores principales: Formenti, Nicoletta, Guarneri, Flavia, Bertasio, Cristina, Parisio, Giovanni, Romeo, Claudia, Scali, Federico, Birbes, Laura, Boniotti, Maria Beatrice, Diegoli, Giuseppe, Candela, Loredana, Romeo, Gianluca Antonio, Pasquali, Paolo, Alborali, Giovanni Loris
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2022
Materias:
Brief Report
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9734789/
https://www.ncbi.nlm.nih.gov/pubmed/36494741
http://dx.doi.org/10.1186/s13756-022-01194-9
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author Formenti, Nicoletta
Guarneri, Flavia
Bertasio, Cristina
Parisio, Giovanni
Romeo, Claudia
Scali, Federico
Birbes, Laura
Boniotti, Maria Beatrice
Diegoli, Giuseppe
Candela, Loredana
Romeo, Gianluca Antonio
Pasquali, Paolo
Alborali, Giovanni Loris
author_facet Formenti, Nicoletta
Guarneri, Flavia
Bertasio, Cristina
Parisio, Giovanni
Romeo, Claudia
Scali, Federico
Birbes, Laura
Boniotti, Maria Beatrice
Diegoli, Giuseppe
Candela, Loredana
Romeo, Gianluca Antonio
Pasquali, Paolo
Alborali, Giovanni Loris
author_sort Formenti, Nicoletta
collection PubMed
description Wastewater-based surveillance enabled the first detection of the mobile colistin resistance gene mcr-10 in Italy. This plasmid-borne resistance gene was found in strains of Klebsiella quasipneumoniae isolated from samples of human raw sewage collected over several months. Although the isolates were phenotypically susceptible to colistin, the emergence of mcr-10 is concerning due to the highly variable expression of the gene and the potential for horizontal transfer to other species. In addition, the strains also carried an extended-spectrum β-lactamase gene and were phenotypically resistant to several beta-lactams. This study highlights the value of wastewater-based surveillance as an effective tool to monitor the emergence of antimicrobial resistance in strains circulating in the community and the environment. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s13756-022-01194-9.
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spelling pubmed-97347892022-12-11 Wastewater-based surveillance in Italy leading to the first detection of mcr-10-positive Klebsiella quasipneumoniae Formenti, Nicoletta Guarneri, Flavia Bertasio, Cristina Parisio, Giovanni Romeo, Claudia Scali, Federico Birbes, Laura Boniotti, Maria Beatrice Diegoli, Giuseppe Candela, Loredana Romeo, Gianluca Antonio Pasquali, Paolo Alborali, Giovanni Loris Antimicrob Resist Infect Control Brief Report Wastewater-based surveillance enabled the first detection of the mobile colistin resistance gene mcr-10 in Italy. This plasmid-borne resistance gene was found in strains of Klebsiella quasipneumoniae isolated from samples of human raw sewage collected over several months. Although the isolates were phenotypically susceptible to colistin, the emergence of mcr-10 is concerning due to the highly variable expression of the gene and the potential for horizontal transfer to other species. In addition, the strains also carried an extended-spectrum β-lactamase gene and were phenotypically resistant to several beta-lactams. This study highlights the value of wastewater-based surveillance as an effective tool to monitor the emergence of antimicrobial resistance in strains circulating in the community and the environment. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s13756-022-01194-9. BioMed Central 2022-12-09 /pmc/articles/PMC9734789/ /pubmed/36494741 http://dx.doi.org/10.1186/s13756-022-01194-9 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Brief Report
Formenti, Nicoletta
Guarneri, Flavia
Bertasio, Cristina
Parisio, Giovanni
Romeo, Claudia
Scali, Federico
Birbes, Laura
Boniotti, Maria Beatrice
Diegoli, Giuseppe
Candela, Loredana
Romeo, Gianluca Antonio
Pasquali, Paolo
Alborali, Giovanni Loris
Wastewater-based surveillance in Italy leading to the first detection of mcr-10-positive Klebsiella quasipneumoniae
title Wastewater-based surveillance in Italy leading to the first detection of mcr-10-positive Klebsiella quasipneumoniae
title_full Wastewater-based surveillance in Italy leading to the first detection of mcr-10-positive Klebsiella quasipneumoniae
title_fullStr Wastewater-based surveillance in Italy leading to the first detection of mcr-10-positive Klebsiella quasipneumoniae
title_full_unstemmed Wastewater-based surveillance in Italy leading to the first detection of mcr-10-positive Klebsiella quasipneumoniae
title_short Wastewater-based surveillance in Italy leading to the first detection of mcr-10-positive Klebsiella quasipneumoniae
title_sort wastewater-based surveillance in italy leading to the first detection of mcr-10-positive klebsiella quasipneumoniae
topic Brief Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9734789/
https://www.ncbi.nlm.nih.gov/pubmed/36494741
http://dx.doi.org/10.1186/s13756-022-01194-9
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