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Label-free ultra-sensitive colorimetric detection of hepatitis E virus based on oxidase-like activity of MnO(2) nanosheets

Hepatitis E virus (HEV) is an evolving infectious entity that causes viral hepatitis infections worldwide. Current routine methods of identifying and diagnosing HEV are someway laborious and costly. Based on the biomimicking oxidase-like activity of MnO(2) nanosheets, we designed a label-free, highl...

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Autores principales: Alam, Naveed, Ravikumar, Chandan Hunsur, Sreeramareddygari, Muralikrishna, Somasundrum, Mithran, Surareungchai, Werasak
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Berlin Heidelberg 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9734815/
https://www.ncbi.nlm.nih.gov/pubmed/36469053
http://dx.doi.org/10.1007/s00216-022-04461-1
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author Alam, Naveed
Ravikumar, Chandan Hunsur
Sreeramareddygari, Muralikrishna
Somasundrum, Mithran
Surareungchai, Werasak
author_facet Alam, Naveed
Ravikumar, Chandan Hunsur
Sreeramareddygari, Muralikrishna
Somasundrum, Mithran
Surareungchai, Werasak
author_sort Alam, Naveed
collection PubMed
description Hepatitis E virus (HEV) is an evolving infectious entity that causes viral hepatitis infections worldwide. Current routine methods of identifying and diagnosing HEV are someway laborious and costly. Based on the biomimicking oxidase-like activity of MnO(2) nanosheets, we designed a label-free, highly sensitive colorimetric sensing technique for HEV detection. The prepared MnO(2) catalyst displays intrinsic biomimicking oxidase-like catalytic activity and efficiently oxidizes the 3,3′,5,5′-tetramethylbenzidine (TMB) substrate from colorless to blue colored oxidized TMB (oxTMB) product which can be measured at 652 nm by UV–visible spectrum. When the HEV-DNA was added, DNA adsorbed easily on MnO(2) surface through physical adsorption and electrostatic interaction which hinders the oxidase-like catalytic activity of MnO(2). Upon the introduction of target, the HEV target DNA binds with its complementary ssDNA on the surface of MnO(2), the hybridized DNA releases from the surface of MnO(2), which leads to recovery of oxidase-like catalytic activity of MnO(2). This strategy was applied to construct a colorimetric technique for HEV detection. The approach works in the linear range of 1 fM–100 nM DNA concentration with the limit of detection (LOD) of 3.26 fM (S/N = 3) and quantitative limit (LOQ) of 36.08 fM. The TMB-MnO(2) platform was highly selective for HEV target DNA detection when compared with potential interferences. Result of serum sample analysis demonstrates that this sensing system can be used for clinical diagnostic applications. GRAPHICAL ABSTRACT: [Image: see text] SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s00216-022-04461-1.
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spelling pubmed-97348152022-12-12 Label-free ultra-sensitive colorimetric detection of hepatitis E virus based on oxidase-like activity of MnO(2) nanosheets Alam, Naveed Ravikumar, Chandan Hunsur Sreeramareddygari, Muralikrishna Somasundrum, Mithran Surareungchai, Werasak Anal Bioanal Chem Research Paper Hepatitis E virus (HEV) is an evolving infectious entity that causes viral hepatitis infections worldwide. Current routine methods of identifying and diagnosing HEV are someway laborious and costly. Based on the biomimicking oxidase-like activity of MnO(2) nanosheets, we designed a label-free, highly sensitive colorimetric sensing technique for HEV detection. The prepared MnO(2) catalyst displays intrinsic biomimicking oxidase-like catalytic activity and efficiently oxidizes the 3,3′,5,5′-tetramethylbenzidine (TMB) substrate from colorless to blue colored oxidized TMB (oxTMB) product which can be measured at 652 nm by UV–visible spectrum. When the HEV-DNA was added, DNA adsorbed easily on MnO(2) surface through physical adsorption and electrostatic interaction which hinders the oxidase-like catalytic activity of MnO(2). Upon the introduction of target, the HEV target DNA binds with its complementary ssDNA on the surface of MnO(2), the hybridized DNA releases from the surface of MnO(2), which leads to recovery of oxidase-like catalytic activity of MnO(2). This strategy was applied to construct a colorimetric technique for HEV detection. The approach works in the linear range of 1 fM–100 nM DNA concentration with the limit of detection (LOD) of 3.26 fM (S/N = 3) and quantitative limit (LOQ) of 36.08 fM. The TMB-MnO(2) platform was highly selective for HEV target DNA detection when compared with potential interferences. Result of serum sample analysis demonstrates that this sensing system can be used for clinical diagnostic applications. GRAPHICAL ABSTRACT: [Image: see text] SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s00216-022-04461-1. Springer Berlin Heidelberg 2022-12-05 2023 /pmc/articles/PMC9734815/ /pubmed/36469053 http://dx.doi.org/10.1007/s00216-022-04461-1 Text en © Springer-Verlag GmbH Germany, part of Springer Nature 2022, Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law. This article is made available via the PMC Open Access Subset for unrestricted research re-use and secondary analysis in any form or by any means with acknowledgement of the original source. These permissions are granted for the duration of the World Health Organization (WHO) declaration of COVID-19 as a global pandemic.
spellingShingle Research Paper
Alam, Naveed
Ravikumar, Chandan Hunsur
Sreeramareddygari, Muralikrishna
Somasundrum, Mithran
Surareungchai, Werasak
Label-free ultra-sensitive colorimetric detection of hepatitis E virus based on oxidase-like activity of MnO(2) nanosheets
title Label-free ultra-sensitive colorimetric detection of hepatitis E virus based on oxidase-like activity of MnO(2) nanosheets
title_full Label-free ultra-sensitive colorimetric detection of hepatitis E virus based on oxidase-like activity of MnO(2) nanosheets
title_fullStr Label-free ultra-sensitive colorimetric detection of hepatitis E virus based on oxidase-like activity of MnO(2) nanosheets
title_full_unstemmed Label-free ultra-sensitive colorimetric detection of hepatitis E virus based on oxidase-like activity of MnO(2) nanosheets
title_short Label-free ultra-sensitive colorimetric detection of hepatitis E virus based on oxidase-like activity of MnO(2) nanosheets
title_sort label-free ultra-sensitive colorimetric detection of hepatitis e virus based on oxidase-like activity of mno(2) nanosheets
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9734815/
https://www.ncbi.nlm.nih.gov/pubmed/36469053
http://dx.doi.org/10.1007/s00216-022-04461-1
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