Cargando…
Comparison of Different Techniques for the Determination of Platinized Cytostatic Drugs in Urine Samples
Platinum-based cytostatic drugs are one of the most widely used cancer treatments. They are excreted via the urinary tract and can reach the environment through wastewater, posing a risk to human health due to their side effects. Four identification and quantification techniques, including liquid ch...
Autores principales: | , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9735434/ https://www.ncbi.nlm.nih.gov/pubmed/36500232 http://dx.doi.org/10.3390/molecules27238139 |
_version_ | 1784846763652284416 |
---|---|
author | Arenas, Marina Martín, Julia Santos, Juan Luis Aparicio, Irene Fernández-Sanfrancisco, Omar Alonso, Esteban |
author_facet | Arenas, Marina Martín, Julia Santos, Juan Luis Aparicio, Irene Fernández-Sanfrancisco, Omar Alonso, Esteban |
author_sort | Arenas, Marina |
collection | PubMed |
description | Platinum-based cytostatic drugs are one of the most widely used cancer treatments. They are excreted via the urinary tract and can reach the environment through wastewater, posing a risk to human health due to their side effects. Four identification and quantification techniques, including liquid chromatography (LC) separation coupled to (i) a diode array ultraviolet (UV(DAD)) (ii), mass spectrometer in single ion monitoring mode (LC-MS) and (iii) multiple reaction monitoring mode (LC-MS/MS) and (iv) derivatization with diethyldithiocarbamate prior to LC-MS/MS analysis, have been optimized and compared for the multiresidue determination of main platinized cytostatic drugs (cisplatin, carboplatin, and oxaliplatin) in urine samples. Parameters that affect the efficiency of the chromatographic separation and analytical determination of different methods (column, mobile phase, wavelength, precursor ions, fragmentor, and product ions) were optimized. Analytical features, such as matrix effect, sensitivity, precision, selectivity, and linearity, were calculated. In terms of selectivity, the derivatization technique was discarded since it was only applicable to the platinated sum. A high dilution of the sample with LC-UV(DAD) was needed to reduce the matrix effect. Overall, the LC-MS/MS method presented the best analytical features (% RSD ≤ 12.8%, R(2) ≥ 0.991, or method-detection limits between 0.01–1 µg mL(−1)). The selected method was applied to the quantification of platinized cytostatic drugs in hospital urine samples from oncologic patients. |
format | Online Article Text |
id | pubmed-9735434 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-97354342022-12-11 Comparison of Different Techniques for the Determination of Platinized Cytostatic Drugs in Urine Samples Arenas, Marina Martín, Julia Santos, Juan Luis Aparicio, Irene Fernández-Sanfrancisco, Omar Alonso, Esteban Molecules Article Platinum-based cytostatic drugs are one of the most widely used cancer treatments. They are excreted via the urinary tract and can reach the environment through wastewater, posing a risk to human health due to their side effects. Four identification and quantification techniques, including liquid chromatography (LC) separation coupled to (i) a diode array ultraviolet (UV(DAD)) (ii), mass spectrometer in single ion monitoring mode (LC-MS) and (iii) multiple reaction monitoring mode (LC-MS/MS) and (iv) derivatization with diethyldithiocarbamate prior to LC-MS/MS analysis, have been optimized and compared for the multiresidue determination of main platinized cytostatic drugs (cisplatin, carboplatin, and oxaliplatin) in urine samples. Parameters that affect the efficiency of the chromatographic separation and analytical determination of different methods (column, mobile phase, wavelength, precursor ions, fragmentor, and product ions) were optimized. Analytical features, such as matrix effect, sensitivity, precision, selectivity, and linearity, were calculated. In terms of selectivity, the derivatization technique was discarded since it was only applicable to the platinated sum. A high dilution of the sample with LC-UV(DAD) was needed to reduce the matrix effect. Overall, the LC-MS/MS method presented the best analytical features (% RSD ≤ 12.8%, R(2) ≥ 0.991, or method-detection limits between 0.01–1 µg mL(−1)). The selected method was applied to the quantification of platinized cytostatic drugs in hospital urine samples from oncologic patients. MDPI 2022-11-23 /pmc/articles/PMC9735434/ /pubmed/36500232 http://dx.doi.org/10.3390/molecules27238139 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Arenas, Marina Martín, Julia Santos, Juan Luis Aparicio, Irene Fernández-Sanfrancisco, Omar Alonso, Esteban Comparison of Different Techniques for the Determination of Platinized Cytostatic Drugs in Urine Samples |
title | Comparison of Different Techniques for the Determination of Platinized Cytostatic Drugs in Urine Samples |
title_full | Comparison of Different Techniques for the Determination of Platinized Cytostatic Drugs in Urine Samples |
title_fullStr | Comparison of Different Techniques for the Determination of Platinized Cytostatic Drugs in Urine Samples |
title_full_unstemmed | Comparison of Different Techniques for the Determination of Platinized Cytostatic Drugs in Urine Samples |
title_short | Comparison of Different Techniques for the Determination of Platinized Cytostatic Drugs in Urine Samples |
title_sort | comparison of different techniques for the determination of platinized cytostatic drugs in urine samples |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9735434/ https://www.ncbi.nlm.nih.gov/pubmed/36500232 http://dx.doi.org/10.3390/molecules27238139 |
work_keys_str_mv | AT arenasmarina comparisonofdifferenttechniquesforthedeterminationofplatinizedcytostaticdrugsinurinesamples AT martinjulia comparisonofdifferenttechniquesforthedeterminationofplatinizedcytostaticdrugsinurinesamples AT santosjuanluis comparisonofdifferenttechniquesforthedeterminationofplatinizedcytostaticdrugsinurinesamples AT aparicioirene comparisonofdifferenttechniquesforthedeterminationofplatinizedcytostaticdrugsinurinesamples AT fernandezsanfranciscoomar comparisonofdifferenttechniquesforthedeterminationofplatinizedcytostaticdrugsinurinesamples AT alonsoesteban comparisonofdifferenttechniquesforthedeterminationofplatinizedcytostaticdrugsinurinesamples |