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Effects of Neutralization on the Physicochemical, Mechanical, and Biological Properties of Ammonium-Hydroxide-Crosslinked Chitosan Scaffolds
It has been reported that chitosan scaffolds, due to their physicochemical properties, stimulate cell proliferation in different tissues of the human body. This study aimed to determine the physicochemical, mechanical, and biological properties of chitosan scaffolds crosslinked with ammonium hydroxi...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9735449/ https://www.ncbi.nlm.nih.gov/pubmed/36499146 http://dx.doi.org/10.3390/ijms232314822 |
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author | Azueta-Aguayo, Paola Hassibe Chuc-Gamboa, Martha Gabriela Aguilar-Pérez, Fernando Javier Aguilar-Ayala, Fernando Javier Rodas-Junco, Beatriz A. Vargas-Coronado, Rossana Faride Cauich-Rodríguez, Juan Valerio |
author_facet | Azueta-Aguayo, Paola Hassibe Chuc-Gamboa, Martha Gabriela Aguilar-Pérez, Fernando Javier Aguilar-Ayala, Fernando Javier Rodas-Junco, Beatriz A. Vargas-Coronado, Rossana Faride Cauich-Rodríguez, Juan Valerio |
author_sort | Azueta-Aguayo, Paola Hassibe |
collection | PubMed |
description | It has been reported that chitosan scaffolds, due to their physicochemical properties, stimulate cell proliferation in different tissues of the human body. This study aimed to determine the physicochemical, mechanical, and biological properties of chitosan scaffolds crosslinked with ammonium hydroxide, with different pH values, to better understand cell behavior depending on the pH of the biomaterial. Scaffolds were either neutralized with sodium hydroxide solution, washed with distilled water until reaching a neutral pH, or kept at alkaline pH. Physicochemical characterization included scanning electron microscopy (SEM), elemental composition (EDX), Fourier-transform infrared (FTIR) spectroscopy, Raman spectroscopy, thermogravimetric analysis (TGA), and mechanical testing. In vitro cytotoxicity was assessed via dental-pulp stem cells’ (DPSCs’) biocompatibility. The results revealed that the neutralized scaffolds exhibited better cell proliferation and morphology. It was concluded that the chitosan scaffolds’ high pH (due to residual ammonium hydroxide) decreases DPSCs’ cell viability. |
format | Online Article Text |
id | pubmed-9735449 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-97354492022-12-11 Effects of Neutralization on the Physicochemical, Mechanical, and Biological Properties of Ammonium-Hydroxide-Crosslinked Chitosan Scaffolds Azueta-Aguayo, Paola Hassibe Chuc-Gamboa, Martha Gabriela Aguilar-Pérez, Fernando Javier Aguilar-Ayala, Fernando Javier Rodas-Junco, Beatriz A. Vargas-Coronado, Rossana Faride Cauich-Rodríguez, Juan Valerio Int J Mol Sci Article It has been reported that chitosan scaffolds, due to their physicochemical properties, stimulate cell proliferation in different tissues of the human body. This study aimed to determine the physicochemical, mechanical, and biological properties of chitosan scaffolds crosslinked with ammonium hydroxide, with different pH values, to better understand cell behavior depending on the pH of the biomaterial. Scaffolds were either neutralized with sodium hydroxide solution, washed with distilled water until reaching a neutral pH, or kept at alkaline pH. Physicochemical characterization included scanning electron microscopy (SEM), elemental composition (EDX), Fourier-transform infrared (FTIR) spectroscopy, Raman spectroscopy, thermogravimetric analysis (TGA), and mechanical testing. In vitro cytotoxicity was assessed via dental-pulp stem cells’ (DPSCs’) biocompatibility. The results revealed that the neutralized scaffolds exhibited better cell proliferation and morphology. It was concluded that the chitosan scaffolds’ high pH (due to residual ammonium hydroxide) decreases DPSCs’ cell viability. MDPI 2022-11-26 /pmc/articles/PMC9735449/ /pubmed/36499146 http://dx.doi.org/10.3390/ijms232314822 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Azueta-Aguayo, Paola Hassibe Chuc-Gamboa, Martha Gabriela Aguilar-Pérez, Fernando Javier Aguilar-Ayala, Fernando Javier Rodas-Junco, Beatriz A. Vargas-Coronado, Rossana Faride Cauich-Rodríguez, Juan Valerio Effects of Neutralization on the Physicochemical, Mechanical, and Biological Properties of Ammonium-Hydroxide-Crosslinked Chitosan Scaffolds |
title | Effects of Neutralization on the Physicochemical, Mechanical, and Biological Properties of Ammonium-Hydroxide-Crosslinked Chitosan Scaffolds |
title_full | Effects of Neutralization on the Physicochemical, Mechanical, and Biological Properties of Ammonium-Hydroxide-Crosslinked Chitosan Scaffolds |
title_fullStr | Effects of Neutralization on the Physicochemical, Mechanical, and Biological Properties of Ammonium-Hydroxide-Crosslinked Chitosan Scaffolds |
title_full_unstemmed | Effects of Neutralization on the Physicochemical, Mechanical, and Biological Properties of Ammonium-Hydroxide-Crosslinked Chitosan Scaffolds |
title_short | Effects of Neutralization on the Physicochemical, Mechanical, and Biological Properties of Ammonium-Hydroxide-Crosslinked Chitosan Scaffolds |
title_sort | effects of neutralization on the physicochemical, mechanical, and biological properties of ammonium-hydroxide-crosslinked chitosan scaffolds |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9735449/ https://www.ncbi.nlm.nih.gov/pubmed/36499146 http://dx.doi.org/10.3390/ijms232314822 |
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