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Effects of Neutralization on the Physicochemical, Mechanical, and Biological Properties of Ammonium-Hydroxide-Crosslinked Chitosan Scaffolds

It has been reported that chitosan scaffolds, due to their physicochemical properties, stimulate cell proliferation in different tissues of the human body. This study aimed to determine the physicochemical, mechanical, and biological properties of chitosan scaffolds crosslinked with ammonium hydroxi...

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Autores principales: Azueta-Aguayo, Paola Hassibe, Chuc-Gamboa, Martha Gabriela, Aguilar-Pérez, Fernando Javier, Aguilar-Ayala, Fernando Javier, Rodas-Junco, Beatriz A., Vargas-Coronado, Rossana Faride, Cauich-Rodríguez, Juan Valerio
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9735449/
https://www.ncbi.nlm.nih.gov/pubmed/36499146
http://dx.doi.org/10.3390/ijms232314822
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author Azueta-Aguayo, Paola Hassibe
Chuc-Gamboa, Martha Gabriela
Aguilar-Pérez, Fernando Javier
Aguilar-Ayala, Fernando Javier
Rodas-Junco, Beatriz A.
Vargas-Coronado, Rossana Faride
Cauich-Rodríguez, Juan Valerio
author_facet Azueta-Aguayo, Paola Hassibe
Chuc-Gamboa, Martha Gabriela
Aguilar-Pérez, Fernando Javier
Aguilar-Ayala, Fernando Javier
Rodas-Junco, Beatriz A.
Vargas-Coronado, Rossana Faride
Cauich-Rodríguez, Juan Valerio
author_sort Azueta-Aguayo, Paola Hassibe
collection PubMed
description It has been reported that chitosan scaffolds, due to their physicochemical properties, stimulate cell proliferation in different tissues of the human body. This study aimed to determine the physicochemical, mechanical, and biological properties of chitosan scaffolds crosslinked with ammonium hydroxide, with different pH values, to better understand cell behavior depending on the pH of the biomaterial. Scaffolds were either neutralized with sodium hydroxide solution, washed with distilled water until reaching a neutral pH, or kept at alkaline pH. Physicochemical characterization included scanning electron microscopy (SEM), elemental composition (EDX), Fourier-transform infrared (FTIR) spectroscopy, Raman spectroscopy, thermogravimetric analysis (TGA), and mechanical testing. In vitro cytotoxicity was assessed via dental-pulp stem cells’ (DPSCs’) biocompatibility. The results revealed that the neutralized scaffolds exhibited better cell proliferation and morphology. It was concluded that the chitosan scaffolds’ high pH (due to residual ammonium hydroxide) decreases DPSCs’ cell viability.
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spelling pubmed-97354492022-12-11 Effects of Neutralization on the Physicochemical, Mechanical, and Biological Properties of Ammonium-Hydroxide-Crosslinked Chitosan Scaffolds Azueta-Aguayo, Paola Hassibe Chuc-Gamboa, Martha Gabriela Aguilar-Pérez, Fernando Javier Aguilar-Ayala, Fernando Javier Rodas-Junco, Beatriz A. Vargas-Coronado, Rossana Faride Cauich-Rodríguez, Juan Valerio Int J Mol Sci Article It has been reported that chitosan scaffolds, due to their physicochemical properties, stimulate cell proliferation in different tissues of the human body. This study aimed to determine the physicochemical, mechanical, and biological properties of chitosan scaffolds crosslinked with ammonium hydroxide, with different pH values, to better understand cell behavior depending on the pH of the biomaterial. Scaffolds were either neutralized with sodium hydroxide solution, washed with distilled water until reaching a neutral pH, or kept at alkaline pH. Physicochemical characterization included scanning electron microscopy (SEM), elemental composition (EDX), Fourier-transform infrared (FTIR) spectroscopy, Raman spectroscopy, thermogravimetric analysis (TGA), and mechanical testing. In vitro cytotoxicity was assessed via dental-pulp stem cells’ (DPSCs’) biocompatibility. The results revealed that the neutralized scaffolds exhibited better cell proliferation and morphology. It was concluded that the chitosan scaffolds’ high pH (due to residual ammonium hydroxide) decreases DPSCs’ cell viability. MDPI 2022-11-26 /pmc/articles/PMC9735449/ /pubmed/36499146 http://dx.doi.org/10.3390/ijms232314822 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Azueta-Aguayo, Paola Hassibe
Chuc-Gamboa, Martha Gabriela
Aguilar-Pérez, Fernando Javier
Aguilar-Ayala, Fernando Javier
Rodas-Junco, Beatriz A.
Vargas-Coronado, Rossana Faride
Cauich-Rodríguez, Juan Valerio
Effects of Neutralization on the Physicochemical, Mechanical, and Biological Properties of Ammonium-Hydroxide-Crosslinked Chitosan Scaffolds
title Effects of Neutralization on the Physicochemical, Mechanical, and Biological Properties of Ammonium-Hydroxide-Crosslinked Chitosan Scaffolds
title_full Effects of Neutralization on the Physicochemical, Mechanical, and Biological Properties of Ammonium-Hydroxide-Crosslinked Chitosan Scaffolds
title_fullStr Effects of Neutralization on the Physicochemical, Mechanical, and Biological Properties of Ammonium-Hydroxide-Crosslinked Chitosan Scaffolds
title_full_unstemmed Effects of Neutralization on the Physicochemical, Mechanical, and Biological Properties of Ammonium-Hydroxide-Crosslinked Chitosan Scaffolds
title_short Effects of Neutralization on the Physicochemical, Mechanical, and Biological Properties of Ammonium-Hydroxide-Crosslinked Chitosan Scaffolds
title_sort effects of neutralization on the physicochemical, mechanical, and biological properties of ammonium-hydroxide-crosslinked chitosan scaffolds
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9735449/
https://www.ncbi.nlm.nih.gov/pubmed/36499146
http://dx.doi.org/10.3390/ijms232314822
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