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Epigallocatechin Gallate Attenuates Gentamicin-Induced Nephrotoxicity by Suppressing Apoptosis and Ferroptosis

Gentamicin (GEN) is a kind of aminoglycoside antibiotic with the adverse effect of nephrotoxicity. Currently, no effective measures against the nephrotoxicity have been approved. In the present study, epigallocatechin gallate (EG), a useful ingredient in green tea, was used to attenuate its nephroto...

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Autores principales: Yue, Lin, Yang, Ya-Ru, Ma, Wen-Xian, Wang, Hong-Yan, Fan, Qian-Wen, Wang, Yue-Yue, Li, Chao, Wang, Jing, Hu, Zi-Mu, Wang, Xue-Fu, Li, Feng-He, Liu, Ming-Ming, Jin, Juan, Shi, Chao, Wen, Jia-Gen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9735461/
https://www.ncbi.nlm.nih.gov/pubmed/36500657
http://dx.doi.org/10.3390/molecules27238564
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author Yue, Lin
Yang, Ya-Ru
Ma, Wen-Xian
Wang, Hong-Yan
Fan, Qian-Wen
Wang, Yue-Yue
Li, Chao
Wang, Jing
Hu, Zi-Mu
Wang, Xue-Fu
Li, Feng-He
Liu, Ming-Ming
Jin, Juan
Shi, Chao
Wen, Jia-Gen
author_facet Yue, Lin
Yang, Ya-Ru
Ma, Wen-Xian
Wang, Hong-Yan
Fan, Qian-Wen
Wang, Yue-Yue
Li, Chao
Wang, Jing
Hu, Zi-Mu
Wang, Xue-Fu
Li, Feng-He
Liu, Ming-Ming
Jin, Juan
Shi, Chao
Wen, Jia-Gen
author_sort Yue, Lin
collection PubMed
description Gentamicin (GEN) is a kind of aminoglycoside antibiotic with the adverse effect of nephrotoxicity. Currently, no effective measures against the nephrotoxicity have been approved. In the present study, epigallocatechin gallate (EG), a useful ingredient in green tea, was used to attenuate its nephrotoxicity. EG was shown to largely attenuate the renal damage and the increase of malondialdehyde (MDA) and the decrease of glutathione (GSH) in GEN-injected rats. In NRK-52E cells, GEN increased the cellular ROS in the early treatment phase and ROS remained continuously high from 1.5 H to 24 H. Moreover, EG alleviated the increase of ROS and MDA and the decrease of GSH caused by GEN. Furthermore, EG activated the protein levels of nuclear factor erythroid 2-related factor 2 (Nrf2) and heme oxygenase-1 (HO-1). After the treatment of GEN, the protein level of cleaved-caspase-3, the flow cytometry analysis and the JC-1 staining, the protein levels of glutathione peroxidase 4 (GPX4) and SLC7A11, were greatly changed, indicating the occurrence of both apoptosis and ferroptosis, whereas EG can reduce these changes. However, when Nrf2 was knocked down by siRNA, the above protective effects of EG were weakened. In summary, EG attenuated GEN-induced nephrotoxicity by suppressing apoptosis and ferroptosis.
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spelling pubmed-97354612022-12-11 Epigallocatechin Gallate Attenuates Gentamicin-Induced Nephrotoxicity by Suppressing Apoptosis and Ferroptosis Yue, Lin Yang, Ya-Ru Ma, Wen-Xian Wang, Hong-Yan Fan, Qian-Wen Wang, Yue-Yue Li, Chao Wang, Jing Hu, Zi-Mu Wang, Xue-Fu Li, Feng-He Liu, Ming-Ming Jin, Juan Shi, Chao Wen, Jia-Gen Molecules Article Gentamicin (GEN) is a kind of aminoglycoside antibiotic with the adverse effect of nephrotoxicity. Currently, no effective measures against the nephrotoxicity have been approved. In the present study, epigallocatechin gallate (EG), a useful ingredient in green tea, was used to attenuate its nephrotoxicity. EG was shown to largely attenuate the renal damage and the increase of malondialdehyde (MDA) and the decrease of glutathione (GSH) in GEN-injected rats. In NRK-52E cells, GEN increased the cellular ROS in the early treatment phase and ROS remained continuously high from 1.5 H to 24 H. Moreover, EG alleviated the increase of ROS and MDA and the decrease of GSH caused by GEN. Furthermore, EG activated the protein levels of nuclear factor erythroid 2-related factor 2 (Nrf2) and heme oxygenase-1 (HO-1). After the treatment of GEN, the protein level of cleaved-caspase-3, the flow cytometry analysis and the JC-1 staining, the protein levels of glutathione peroxidase 4 (GPX4) and SLC7A11, were greatly changed, indicating the occurrence of both apoptosis and ferroptosis, whereas EG can reduce these changes. However, when Nrf2 was knocked down by siRNA, the above protective effects of EG were weakened. In summary, EG attenuated GEN-induced nephrotoxicity by suppressing apoptosis and ferroptosis. MDPI 2022-12-05 /pmc/articles/PMC9735461/ /pubmed/36500657 http://dx.doi.org/10.3390/molecules27238564 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Yue, Lin
Yang, Ya-Ru
Ma, Wen-Xian
Wang, Hong-Yan
Fan, Qian-Wen
Wang, Yue-Yue
Li, Chao
Wang, Jing
Hu, Zi-Mu
Wang, Xue-Fu
Li, Feng-He
Liu, Ming-Ming
Jin, Juan
Shi, Chao
Wen, Jia-Gen
Epigallocatechin Gallate Attenuates Gentamicin-Induced Nephrotoxicity by Suppressing Apoptosis and Ferroptosis
title Epigallocatechin Gallate Attenuates Gentamicin-Induced Nephrotoxicity by Suppressing Apoptosis and Ferroptosis
title_full Epigallocatechin Gallate Attenuates Gentamicin-Induced Nephrotoxicity by Suppressing Apoptosis and Ferroptosis
title_fullStr Epigallocatechin Gallate Attenuates Gentamicin-Induced Nephrotoxicity by Suppressing Apoptosis and Ferroptosis
title_full_unstemmed Epigallocatechin Gallate Attenuates Gentamicin-Induced Nephrotoxicity by Suppressing Apoptosis and Ferroptosis
title_short Epigallocatechin Gallate Attenuates Gentamicin-Induced Nephrotoxicity by Suppressing Apoptosis and Ferroptosis
title_sort epigallocatechin gallate attenuates gentamicin-induced nephrotoxicity by suppressing apoptosis and ferroptosis
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9735461/
https://www.ncbi.nlm.nih.gov/pubmed/36500657
http://dx.doi.org/10.3390/molecules27238564
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