Cargando…

Appraisal of the Antioxidant Activity, Polyphenolic Content, and Characterization of Selected Himalayan Herbs: Anti-Proliferative Potential in HepG2 Cells

Natural antioxidants derived from plants have played a vital role in preventing a wide range of human chronic conditions and provide novel bioactive leads for investigators in pharmacotherapy discovery. This work was designed to examine the ethnopharmacological role of Urtica dioica (UD), Capsella b...

Descripción completa

Detalles Bibliográficos
Autores principales: Yousuf, Sumaira, Shabir, Shabnam, Kauts, Simran, Minocha, Tarun, Obaid, Ahmad A., Khan, Anmar A., Mujalli, Abdulrahman, Jamous, Yahya F., Almaghrabi, Sarah, Baothman, Bandar K., Hjazi, Ahmed, Singh, Sandeep K., Vamanu, Emanuel, Singh, Mahendra P.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9735473/
https://www.ncbi.nlm.nih.gov/pubmed/36500720
http://dx.doi.org/10.3390/molecules27238629
_version_ 1784846773487927296
author Yousuf, Sumaira
Shabir, Shabnam
Kauts, Simran
Minocha, Tarun
Obaid, Ahmad A.
Khan, Anmar A.
Mujalli, Abdulrahman
Jamous, Yahya F.
Almaghrabi, Sarah
Baothman, Bandar K.
Hjazi, Ahmed
Singh, Sandeep K.
Vamanu, Emanuel
Singh, Mahendra P.
author_facet Yousuf, Sumaira
Shabir, Shabnam
Kauts, Simran
Minocha, Tarun
Obaid, Ahmad A.
Khan, Anmar A.
Mujalli, Abdulrahman
Jamous, Yahya F.
Almaghrabi, Sarah
Baothman, Bandar K.
Hjazi, Ahmed
Singh, Sandeep K.
Vamanu, Emanuel
Singh, Mahendra P.
author_sort Yousuf, Sumaira
collection PubMed
description Natural antioxidants derived from plants have played a vital role in preventing a wide range of human chronic conditions and provide novel bioactive leads for investigators in pharmacotherapy discovery. This work was designed to examine the ethnopharmacological role of Urtica dioica (UD), Capsella bursa-pastoris (CBP), and Inula racemosa (IR). The total phenolic and flavonoid contents (TPC and TFC) were illustrated through colorimetric assays, while the antioxidant activity was investigated through DPPH and ABTS assays. The evaluation of phytochemicals by FT-IR of UD and CBP revealed high contents of aliphatic amines, while IR showed a major peak for ketones. The antioxidant activity, TPC and TFC were highest in the ethanol extract of UD, followed by CBP, and IR showed the lowest activity. All of the extracts revealed significant antioxidant capacities along a dosage gradient. Through a HPLC analysis at a wavelength of 280 nm, UD leaves demonstrated an intense peak of quercetin, and the peak for rutin was less intense. CBP (whole plant), instead, demonstrated a major yield of rutin, and a peak for quercetin was not observed in CBP. IR (rhizomes) showed both quercetin and rutin. All of the extracts were significantly cytotoxic to HepG2 cells after 48 h with the trend IR > UD > CBP. The outcomes of this study may be effective in the selection of specific plants as realistic sources of the bioactive components that might be useful in the nutraceutical progression and other biomedical efficacies.
format Online
Article
Text
id pubmed-9735473
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-97354732022-12-11 Appraisal of the Antioxidant Activity, Polyphenolic Content, and Characterization of Selected Himalayan Herbs: Anti-Proliferative Potential in HepG2 Cells Yousuf, Sumaira Shabir, Shabnam Kauts, Simran Minocha, Tarun Obaid, Ahmad A. Khan, Anmar A. Mujalli, Abdulrahman Jamous, Yahya F. Almaghrabi, Sarah Baothman, Bandar K. Hjazi, Ahmed Singh, Sandeep K. Vamanu, Emanuel Singh, Mahendra P. Molecules Article Natural antioxidants derived from plants have played a vital role in preventing a wide range of human chronic conditions and provide novel bioactive leads for investigators in pharmacotherapy discovery. This work was designed to examine the ethnopharmacological role of Urtica dioica (UD), Capsella bursa-pastoris (CBP), and Inula racemosa (IR). The total phenolic and flavonoid contents (TPC and TFC) were illustrated through colorimetric assays, while the antioxidant activity was investigated through DPPH and ABTS assays. The evaluation of phytochemicals by FT-IR of UD and CBP revealed high contents of aliphatic amines, while IR showed a major peak for ketones. The antioxidant activity, TPC and TFC were highest in the ethanol extract of UD, followed by CBP, and IR showed the lowest activity. All of the extracts revealed significant antioxidant capacities along a dosage gradient. Through a HPLC analysis at a wavelength of 280 nm, UD leaves demonstrated an intense peak of quercetin, and the peak for rutin was less intense. CBP (whole plant), instead, demonstrated a major yield of rutin, and a peak for quercetin was not observed in CBP. IR (rhizomes) showed both quercetin and rutin. All of the extracts were significantly cytotoxic to HepG2 cells after 48 h with the trend IR > UD > CBP. The outcomes of this study may be effective in the selection of specific plants as realistic sources of the bioactive components that might be useful in the nutraceutical progression and other biomedical efficacies. MDPI 2022-12-06 /pmc/articles/PMC9735473/ /pubmed/36500720 http://dx.doi.org/10.3390/molecules27238629 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Yousuf, Sumaira
Shabir, Shabnam
Kauts, Simran
Minocha, Tarun
Obaid, Ahmad A.
Khan, Anmar A.
Mujalli, Abdulrahman
Jamous, Yahya F.
Almaghrabi, Sarah
Baothman, Bandar K.
Hjazi, Ahmed
Singh, Sandeep K.
Vamanu, Emanuel
Singh, Mahendra P.
Appraisal of the Antioxidant Activity, Polyphenolic Content, and Characterization of Selected Himalayan Herbs: Anti-Proliferative Potential in HepG2 Cells
title Appraisal of the Antioxidant Activity, Polyphenolic Content, and Characterization of Selected Himalayan Herbs: Anti-Proliferative Potential in HepG2 Cells
title_full Appraisal of the Antioxidant Activity, Polyphenolic Content, and Characterization of Selected Himalayan Herbs: Anti-Proliferative Potential in HepG2 Cells
title_fullStr Appraisal of the Antioxidant Activity, Polyphenolic Content, and Characterization of Selected Himalayan Herbs: Anti-Proliferative Potential in HepG2 Cells
title_full_unstemmed Appraisal of the Antioxidant Activity, Polyphenolic Content, and Characterization of Selected Himalayan Herbs: Anti-Proliferative Potential in HepG2 Cells
title_short Appraisal of the Antioxidant Activity, Polyphenolic Content, and Characterization of Selected Himalayan Herbs: Anti-Proliferative Potential in HepG2 Cells
title_sort appraisal of the antioxidant activity, polyphenolic content, and characterization of selected himalayan herbs: anti-proliferative potential in hepg2 cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9735473/
https://www.ncbi.nlm.nih.gov/pubmed/36500720
http://dx.doi.org/10.3390/molecules27238629
work_keys_str_mv AT yousufsumaira appraisaloftheantioxidantactivitypolyphenoliccontentandcharacterizationofselectedhimalayanherbsantiproliferativepotentialinhepg2cells
AT shabirshabnam appraisaloftheantioxidantactivitypolyphenoliccontentandcharacterizationofselectedhimalayanherbsantiproliferativepotentialinhepg2cells
AT kautssimran appraisaloftheantioxidantactivitypolyphenoliccontentandcharacterizationofselectedhimalayanherbsantiproliferativepotentialinhepg2cells
AT minochatarun appraisaloftheantioxidantactivitypolyphenoliccontentandcharacterizationofselectedhimalayanherbsantiproliferativepotentialinhepg2cells
AT obaidahmada appraisaloftheantioxidantactivitypolyphenoliccontentandcharacterizationofselectedhimalayanherbsantiproliferativepotentialinhepg2cells
AT khananmara appraisaloftheantioxidantactivitypolyphenoliccontentandcharacterizationofselectedhimalayanherbsantiproliferativepotentialinhepg2cells
AT mujalliabdulrahman appraisaloftheantioxidantactivitypolyphenoliccontentandcharacterizationofselectedhimalayanherbsantiproliferativepotentialinhepg2cells
AT jamousyahyaf appraisaloftheantioxidantactivitypolyphenoliccontentandcharacterizationofselectedhimalayanherbsantiproliferativepotentialinhepg2cells
AT almaghrabisarah appraisaloftheantioxidantactivitypolyphenoliccontentandcharacterizationofselectedhimalayanherbsantiproliferativepotentialinhepg2cells
AT baothmanbandark appraisaloftheantioxidantactivitypolyphenoliccontentandcharacterizationofselectedhimalayanherbsantiproliferativepotentialinhepg2cells
AT hjaziahmed appraisaloftheantioxidantactivitypolyphenoliccontentandcharacterizationofselectedhimalayanherbsantiproliferativepotentialinhepg2cells
AT singhsandeepk appraisaloftheantioxidantactivitypolyphenoliccontentandcharacterizationofselectedhimalayanherbsantiproliferativepotentialinhepg2cells
AT vamanuemanuel appraisaloftheantioxidantactivitypolyphenoliccontentandcharacterizationofselectedhimalayanherbsantiproliferativepotentialinhepg2cells
AT singhmahendrap appraisaloftheantioxidantactivitypolyphenoliccontentandcharacterizationofselectedhimalayanherbsantiproliferativepotentialinhepg2cells