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Appraisal of the Antioxidant Activity, Polyphenolic Content, and Characterization of Selected Himalayan Herbs: Anti-Proliferative Potential in HepG2 Cells
Natural antioxidants derived from plants have played a vital role in preventing a wide range of human chronic conditions and provide novel bioactive leads for investigators in pharmacotherapy discovery. This work was designed to examine the ethnopharmacological role of Urtica dioica (UD), Capsella b...
Autores principales: | , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9735473/ https://www.ncbi.nlm.nih.gov/pubmed/36500720 http://dx.doi.org/10.3390/molecules27238629 |
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author | Yousuf, Sumaira Shabir, Shabnam Kauts, Simran Minocha, Tarun Obaid, Ahmad A. Khan, Anmar A. Mujalli, Abdulrahman Jamous, Yahya F. Almaghrabi, Sarah Baothman, Bandar K. Hjazi, Ahmed Singh, Sandeep K. Vamanu, Emanuel Singh, Mahendra P. |
author_facet | Yousuf, Sumaira Shabir, Shabnam Kauts, Simran Minocha, Tarun Obaid, Ahmad A. Khan, Anmar A. Mujalli, Abdulrahman Jamous, Yahya F. Almaghrabi, Sarah Baothman, Bandar K. Hjazi, Ahmed Singh, Sandeep K. Vamanu, Emanuel Singh, Mahendra P. |
author_sort | Yousuf, Sumaira |
collection | PubMed |
description | Natural antioxidants derived from plants have played a vital role in preventing a wide range of human chronic conditions and provide novel bioactive leads for investigators in pharmacotherapy discovery. This work was designed to examine the ethnopharmacological role of Urtica dioica (UD), Capsella bursa-pastoris (CBP), and Inula racemosa (IR). The total phenolic and flavonoid contents (TPC and TFC) were illustrated through colorimetric assays, while the antioxidant activity was investigated through DPPH and ABTS assays. The evaluation of phytochemicals by FT-IR of UD and CBP revealed high contents of aliphatic amines, while IR showed a major peak for ketones. The antioxidant activity, TPC and TFC were highest in the ethanol extract of UD, followed by CBP, and IR showed the lowest activity. All of the extracts revealed significant antioxidant capacities along a dosage gradient. Through a HPLC analysis at a wavelength of 280 nm, UD leaves demonstrated an intense peak of quercetin, and the peak for rutin was less intense. CBP (whole plant), instead, demonstrated a major yield of rutin, and a peak for quercetin was not observed in CBP. IR (rhizomes) showed both quercetin and rutin. All of the extracts were significantly cytotoxic to HepG2 cells after 48 h with the trend IR > UD > CBP. The outcomes of this study may be effective in the selection of specific plants as realistic sources of the bioactive components that might be useful in the nutraceutical progression and other biomedical efficacies. |
format | Online Article Text |
id | pubmed-9735473 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-97354732022-12-11 Appraisal of the Antioxidant Activity, Polyphenolic Content, and Characterization of Selected Himalayan Herbs: Anti-Proliferative Potential in HepG2 Cells Yousuf, Sumaira Shabir, Shabnam Kauts, Simran Minocha, Tarun Obaid, Ahmad A. Khan, Anmar A. Mujalli, Abdulrahman Jamous, Yahya F. Almaghrabi, Sarah Baothman, Bandar K. Hjazi, Ahmed Singh, Sandeep K. Vamanu, Emanuel Singh, Mahendra P. Molecules Article Natural antioxidants derived from plants have played a vital role in preventing a wide range of human chronic conditions and provide novel bioactive leads for investigators in pharmacotherapy discovery. This work was designed to examine the ethnopharmacological role of Urtica dioica (UD), Capsella bursa-pastoris (CBP), and Inula racemosa (IR). The total phenolic and flavonoid contents (TPC and TFC) were illustrated through colorimetric assays, while the antioxidant activity was investigated through DPPH and ABTS assays. The evaluation of phytochemicals by FT-IR of UD and CBP revealed high contents of aliphatic amines, while IR showed a major peak for ketones. The antioxidant activity, TPC and TFC were highest in the ethanol extract of UD, followed by CBP, and IR showed the lowest activity. All of the extracts revealed significant antioxidant capacities along a dosage gradient. Through a HPLC analysis at a wavelength of 280 nm, UD leaves demonstrated an intense peak of quercetin, and the peak for rutin was less intense. CBP (whole plant), instead, demonstrated a major yield of rutin, and a peak for quercetin was not observed in CBP. IR (rhizomes) showed both quercetin and rutin. All of the extracts were significantly cytotoxic to HepG2 cells after 48 h with the trend IR > UD > CBP. The outcomes of this study may be effective in the selection of specific plants as realistic sources of the bioactive components that might be useful in the nutraceutical progression and other biomedical efficacies. MDPI 2022-12-06 /pmc/articles/PMC9735473/ /pubmed/36500720 http://dx.doi.org/10.3390/molecules27238629 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Yousuf, Sumaira Shabir, Shabnam Kauts, Simran Minocha, Tarun Obaid, Ahmad A. Khan, Anmar A. Mujalli, Abdulrahman Jamous, Yahya F. Almaghrabi, Sarah Baothman, Bandar K. Hjazi, Ahmed Singh, Sandeep K. Vamanu, Emanuel Singh, Mahendra P. Appraisal of the Antioxidant Activity, Polyphenolic Content, and Characterization of Selected Himalayan Herbs: Anti-Proliferative Potential in HepG2 Cells |
title | Appraisal of the Antioxidant Activity, Polyphenolic Content, and Characterization of Selected Himalayan Herbs: Anti-Proliferative Potential in HepG2 Cells |
title_full | Appraisal of the Antioxidant Activity, Polyphenolic Content, and Characterization of Selected Himalayan Herbs: Anti-Proliferative Potential in HepG2 Cells |
title_fullStr | Appraisal of the Antioxidant Activity, Polyphenolic Content, and Characterization of Selected Himalayan Herbs: Anti-Proliferative Potential in HepG2 Cells |
title_full_unstemmed | Appraisal of the Antioxidant Activity, Polyphenolic Content, and Characterization of Selected Himalayan Herbs: Anti-Proliferative Potential in HepG2 Cells |
title_short | Appraisal of the Antioxidant Activity, Polyphenolic Content, and Characterization of Selected Himalayan Herbs: Anti-Proliferative Potential in HepG2 Cells |
title_sort | appraisal of the antioxidant activity, polyphenolic content, and characterization of selected himalayan herbs: anti-proliferative potential in hepg2 cells |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9735473/ https://www.ncbi.nlm.nih.gov/pubmed/36500720 http://dx.doi.org/10.3390/molecules27238629 |
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