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Cerium Oxide Nanorods Synthesized by Dalbergia sissoo Extract for Antioxidant, Cytotoxicity, and Photocatalytic Applications

In this study, cerium oxide nanorods (CeO(2)-NRs) were synthesized by using the phytochemicals present in the Dalbergia sissoo extract. The physiochemical characteristics of the as-prepared CeO(2)-NRs were investigated by using ultraviolet-visible spectroscopy (UV-VIS), scanning electron microscopy...

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Autores principales: Alam, Mir Waqas, Naeem, Sumaira, Usman, Sheikh Muhammad, Kanwal, Qudsia, BaQais, Amal, Aldughaylibi, Fatimah Saeed, Nahvi, Insha, Zaidi, Noushi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9735491/
https://www.ncbi.nlm.nih.gov/pubmed/36500279
http://dx.doi.org/10.3390/molecules27238188
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author Alam, Mir Waqas
Naeem, Sumaira
Usman, Sheikh Muhammad
Kanwal, Qudsia
BaQais, Amal
Aldughaylibi, Fatimah Saeed
Nahvi, Insha
Zaidi, Noushi
author_facet Alam, Mir Waqas
Naeem, Sumaira
Usman, Sheikh Muhammad
Kanwal, Qudsia
BaQais, Amal
Aldughaylibi, Fatimah Saeed
Nahvi, Insha
Zaidi, Noushi
author_sort Alam, Mir Waqas
collection PubMed
description In this study, cerium oxide nanorods (CeO(2)-NRs) were synthesized by using the phytochemicals present in the Dalbergia sissoo extract. The physiochemical characteristics of the as-prepared CeO(2)-NRs were investigated by using ultraviolet-visible spectroscopy (UV-VIS), scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR), and X-ray diffraction analysis (XRD). The SEM and UV-VIS analyses revealed that the acquired nanomaterials possessed a rod-like morphology while the XRD results further confirmed that the synthesized NRs exhibited a cubic crystal lattice system. The antioxidant capacity of the synthesized CeO(2)-NRs was investigated by using several in vitro biochemical assays. It was observed that the synthesized NRs exhibited better antioxidant potential in comparison to the industrial antioxidant of the butylated hydroxyanisole (BHA) in 1,1-diphenyl-2-picrylhydrazyl (DPPH) assay. The biochemical assays, including lipid peroxidation (LPO), total antioxidant capacity (TAC), and catalase activity (CAT), were also performed in the human lymphocytes incubated with the CeO(2)-NRs to investigate the impact of the NRs on these oxidative biomarkers. Enhanced reductive capabilities were observed in all the assays, revealing that the NRs possess excellent antioxidant properties. Moreover, the cytotoxic potential of the CeO(2)-NRs was also investigated with the MTT assay. The CeO(2)-NRs were found to effectively kill off the cancerous cells (MCF-7 human breast cancer cell line), further indicating that the synthesized NRs exhibit anticancer potential as well. One of the major applications studied for the prepared CeO(2)-NRs was performing the statistical optimization of the photocatalytic degradation reaction of the methyl orange (MO) dye. The reaction was optimized by using the technique of response surface methodology (RSM). This advanced approach facilitates the development of the predictive model on the basis of central composite design (CCD) for this degradation reaction. The maximum degradation of 99.31% was achieved at the experimental optimized conditions, which corresponded rather well with the predicted percentage degradation values of 99.58%. These results indicate that the developed predictive model can effectively explain the performed experimental reaction. To conclude, the CeO(2)-NRs exhibited excellent results for multiple applications.
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spelling pubmed-97354912022-12-11 Cerium Oxide Nanorods Synthesized by Dalbergia sissoo Extract for Antioxidant, Cytotoxicity, and Photocatalytic Applications Alam, Mir Waqas Naeem, Sumaira Usman, Sheikh Muhammad Kanwal, Qudsia BaQais, Amal Aldughaylibi, Fatimah Saeed Nahvi, Insha Zaidi, Noushi Molecules Article In this study, cerium oxide nanorods (CeO(2)-NRs) were synthesized by using the phytochemicals present in the Dalbergia sissoo extract. The physiochemical characteristics of the as-prepared CeO(2)-NRs were investigated by using ultraviolet-visible spectroscopy (UV-VIS), scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR), and X-ray diffraction analysis (XRD). The SEM and UV-VIS analyses revealed that the acquired nanomaterials possessed a rod-like morphology while the XRD results further confirmed that the synthesized NRs exhibited a cubic crystal lattice system. The antioxidant capacity of the synthesized CeO(2)-NRs was investigated by using several in vitro biochemical assays. It was observed that the synthesized NRs exhibited better antioxidant potential in comparison to the industrial antioxidant of the butylated hydroxyanisole (BHA) in 1,1-diphenyl-2-picrylhydrazyl (DPPH) assay. The biochemical assays, including lipid peroxidation (LPO), total antioxidant capacity (TAC), and catalase activity (CAT), were also performed in the human lymphocytes incubated with the CeO(2)-NRs to investigate the impact of the NRs on these oxidative biomarkers. Enhanced reductive capabilities were observed in all the assays, revealing that the NRs possess excellent antioxidant properties. Moreover, the cytotoxic potential of the CeO(2)-NRs was also investigated with the MTT assay. The CeO(2)-NRs were found to effectively kill off the cancerous cells (MCF-7 human breast cancer cell line), further indicating that the synthesized NRs exhibit anticancer potential as well. One of the major applications studied for the prepared CeO(2)-NRs was performing the statistical optimization of the photocatalytic degradation reaction of the methyl orange (MO) dye. The reaction was optimized by using the technique of response surface methodology (RSM). This advanced approach facilitates the development of the predictive model on the basis of central composite design (CCD) for this degradation reaction. The maximum degradation of 99.31% was achieved at the experimental optimized conditions, which corresponded rather well with the predicted percentage degradation values of 99.58%. These results indicate that the developed predictive model can effectively explain the performed experimental reaction. To conclude, the CeO(2)-NRs exhibited excellent results for multiple applications. MDPI 2022-11-24 /pmc/articles/PMC9735491/ /pubmed/36500279 http://dx.doi.org/10.3390/molecules27238188 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Alam, Mir Waqas
Naeem, Sumaira
Usman, Sheikh Muhammad
Kanwal, Qudsia
BaQais, Amal
Aldughaylibi, Fatimah Saeed
Nahvi, Insha
Zaidi, Noushi
Cerium Oxide Nanorods Synthesized by Dalbergia sissoo Extract for Antioxidant, Cytotoxicity, and Photocatalytic Applications
title Cerium Oxide Nanorods Synthesized by Dalbergia sissoo Extract for Antioxidant, Cytotoxicity, and Photocatalytic Applications
title_full Cerium Oxide Nanorods Synthesized by Dalbergia sissoo Extract for Antioxidant, Cytotoxicity, and Photocatalytic Applications
title_fullStr Cerium Oxide Nanorods Synthesized by Dalbergia sissoo Extract for Antioxidant, Cytotoxicity, and Photocatalytic Applications
title_full_unstemmed Cerium Oxide Nanorods Synthesized by Dalbergia sissoo Extract for Antioxidant, Cytotoxicity, and Photocatalytic Applications
title_short Cerium Oxide Nanorods Synthesized by Dalbergia sissoo Extract for Antioxidant, Cytotoxicity, and Photocatalytic Applications
title_sort cerium oxide nanorods synthesized by dalbergia sissoo extract for antioxidant, cytotoxicity, and photocatalytic applications
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9735491/
https://www.ncbi.nlm.nih.gov/pubmed/36500279
http://dx.doi.org/10.3390/molecules27238188
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