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Discovery of 16-Androstenes (Androstenone and Androstenol), Their Synthesis Pathway, and Possible Role in Reproduction of Mouse Deer (Moschiola indica)

We discovered odorous 16-androstenes (Androstenone and Androstenol) in endangered mouse deer during a captive breeding program. This study examined the molecular characteristics, their synthesis pathway, and the possible functional role of these compounds in the reproduction of mouse deer. CYP17A1 a...

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Autores principales: Kumar, Vinod, Manu, Shivakumara, Caroline, Karunakaran, Sekhar, Anupama, Mamta, Sajwan-Khatri, Sandeep, Mushkam, , Wasimuddin, Senthilkumaran, Balasubramanian, Umapathy, Govindhaswamy
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9735587/
http://dx.doi.org/10.3390/cells11233837
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author Kumar, Vinod
Manu, Shivakumara
Caroline, Karunakaran
Sekhar, Anupama
Mamta, Sajwan-Khatri
Sandeep, Mushkam
, Wasimuddin
Senthilkumaran, Balasubramanian
Umapathy, Govindhaswamy
author_facet Kumar, Vinod
Manu, Shivakumara
Caroline, Karunakaran
Sekhar, Anupama
Mamta, Sajwan-Khatri
Sandeep, Mushkam
, Wasimuddin
Senthilkumaran, Balasubramanian
Umapathy, Govindhaswamy
author_sort Kumar, Vinod
collection PubMed
description We discovered odorous 16-androstenes (Androstenone and Androstenol) in endangered mouse deer during a captive breeding program. This study examined the molecular characteristics, their synthesis pathway, and the possible functional role of these compounds in the reproduction of mouse deer. CYP17A1 and CYB5 genes were cloned and expressed in HEK-293, COS-7 cell lines, and gonads of mouse deer to investigate the CYP17A1 gene’s andien-β-synthase activity towards the synthesis of 16-androstenes in mouse deer. An enzyme immunoassay was further developed and standardized to measure fecal androstenone during the reproductive cycles of mouse deer. Results showed that the mouse deer CYP17A1 gene possesses andien-β-synthase activity and could transform pregnenolone into 5,16-androstadien-3β-ol. The expression of the CYP17A1 gene upregulated in the testis and ovary compared to other tissues in mouse deer. Significantly elevated androstenone and estrogens were recorded prior to delivery and postpartum estrus/mating in mouse deer. Further, there were weak correlations between fecal androstenone and estrogens/androgens in mouse deer during the breeding season. These findings suggest that androstenone probably plays a role in the reproductive activities of mouse deer. This knowledge can be used for captive breeding programs of mouse deer in India and elsewhere.
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spelling pubmed-97355872022-12-11 Discovery of 16-Androstenes (Androstenone and Androstenol), Their Synthesis Pathway, and Possible Role in Reproduction of Mouse Deer (Moschiola indica) Kumar, Vinod Manu, Shivakumara Caroline, Karunakaran Sekhar, Anupama Mamta, Sajwan-Khatri Sandeep, Mushkam , Wasimuddin Senthilkumaran, Balasubramanian Umapathy, Govindhaswamy Cells Article We discovered odorous 16-androstenes (Androstenone and Androstenol) in endangered mouse deer during a captive breeding program. This study examined the molecular characteristics, their synthesis pathway, and the possible functional role of these compounds in the reproduction of mouse deer. CYP17A1 and CYB5 genes were cloned and expressed in HEK-293, COS-7 cell lines, and gonads of mouse deer to investigate the CYP17A1 gene’s andien-β-synthase activity towards the synthesis of 16-androstenes in mouse deer. An enzyme immunoassay was further developed and standardized to measure fecal androstenone during the reproductive cycles of mouse deer. Results showed that the mouse deer CYP17A1 gene possesses andien-β-synthase activity and could transform pregnenolone into 5,16-androstadien-3β-ol. The expression of the CYP17A1 gene upregulated in the testis and ovary compared to other tissues in mouse deer. Significantly elevated androstenone and estrogens were recorded prior to delivery and postpartum estrus/mating in mouse deer. Further, there were weak correlations between fecal androstenone and estrogens/androgens in mouse deer during the breeding season. These findings suggest that androstenone probably plays a role in the reproductive activities of mouse deer. This knowledge can be used for captive breeding programs of mouse deer in India and elsewhere. MDPI 2022-11-29 /pmc/articles/PMC9735587/ http://dx.doi.org/10.3390/cells11233837 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Kumar, Vinod
Manu, Shivakumara
Caroline, Karunakaran
Sekhar, Anupama
Mamta, Sajwan-Khatri
Sandeep, Mushkam
, Wasimuddin
Senthilkumaran, Balasubramanian
Umapathy, Govindhaswamy
Discovery of 16-Androstenes (Androstenone and Androstenol), Their Synthesis Pathway, and Possible Role in Reproduction of Mouse Deer (Moschiola indica)
title Discovery of 16-Androstenes (Androstenone and Androstenol), Their Synthesis Pathway, and Possible Role in Reproduction of Mouse Deer (Moschiola indica)
title_full Discovery of 16-Androstenes (Androstenone and Androstenol), Their Synthesis Pathway, and Possible Role in Reproduction of Mouse Deer (Moschiola indica)
title_fullStr Discovery of 16-Androstenes (Androstenone and Androstenol), Their Synthesis Pathway, and Possible Role in Reproduction of Mouse Deer (Moschiola indica)
title_full_unstemmed Discovery of 16-Androstenes (Androstenone and Androstenol), Their Synthesis Pathway, and Possible Role in Reproduction of Mouse Deer (Moschiola indica)
title_short Discovery of 16-Androstenes (Androstenone and Androstenol), Their Synthesis Pathway, and Possible Role in Reproduction of Mouse Deer (Moschiola indica)
title_sort discovery of 16-androstenes (androstenone and androstenol), their synthesis pathway, and possible role in reproduction of mouse deer (moschiola indica)
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9735587/
http://dx.doi.org/10.3390/cells11233837
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