Cargando…

Comparative Transcriptome Analysis of CCCH Family in Roles of Flower Opening and Abiotic Stress in Osmanthus fragrans

CCCH is a zinc finger family with a typical CCCH-type motif which performs a variety of roles in plant growth and development and responses to environmental stressors. However, the information about this family has not been reported for Osmanthus fragrans. In this study, a total of 66 CCCH predicted...

Descripción completa

Detalles Bibliográficos
Autores principales: Ye, Yong, Cao, Shanshan, Shen, Lixiao, Wang, Yiguang, Zhong, Shiwei, Yang, Liyuan, Xiao, Zheng, Fang, Qiu, Zhao, Hongbo, Dong, Bin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9735588/
https://www.ncbi.nlm.nih.gov/pubmed/36499688
http://dx.doi.org/10.3390/ijms232315363
_version_ 1784846806356590592
author Ye, Yong
Cao, Shanshan
Shen, Lixiao
Wang, Yiguang
Zhong, Shiwei
Yang, Liyuan
Xiao, Zheng
Fang, Qiu
Zhao, Hongbo
Dong, Bin
author_facet Ye, Yong
Cao, Shanshan
Shen, Lixiao
Wang, Yiguang
Zhong, Shiwei
Yang, Liyuan
Xiao, Zheng
Fang, Qiu
Zhao, Hongbo
Dong, Bin
author_sort Ye, Yong
collection PubMed
description CCCH is a zinc finger family with a typical CCCH-type motif which performs a variety of roles in plant growth and development and responses to environmental stressors. However, the information about this family has not been reported for Osmanthus fragrans. In this study, a total of 66 CCCH predicted genes were identified from the O. fragrans genome, the majority of which had multiple CCCH motifs. The 66 OfCCCHs were found to be unevenly distributed on 21 chromosomes and were clustered into nine groups based on their phylogenetic analysis. In each group, the gene structure and domain makeup were comparatively conserved. The expression profiles of the OfCCCH genes were examined in various tissues, the flower-opening processes, and under various abiotic stresses using transcriptome sequencing and qRT-PCR (quantitative real-time PCR). The results demonstrated the widespread expression of OfCCCHs in various tissues, the differential expression of 22 OfCCCHs during flower-opening stages, and the identification of 4, 5, and 13 OfCCCHs after ABA, salt, and drought stress treatment, respectively. Furthermore, characterization of the representative OfCCCHs (OfCCCH8, 23, 27, and 36) revealed that they were all localized in the nucleus and that the majority of them had transcriptional activation in the yeast system. Our research offers the first thorough examination of the OfCCCH family and lays the groundwork for future investigations regarding the functions of CCCH genes in O. fragrans.
format Online
Article
Text
id pubmed-9735588
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-97355882022-12-11 Comparative Transcriptome Analysis of CCCH Family in Roles of Flower Opening and Abiotic Stress in Osmanthus fragrans Ye, Yong Cao, Shanshan Shen, Lixiao Wang, Yiguang Zhong, Shiwei Yang, Liyuan Xiao, Zheng Fang, Qiu Zhao, Hongbo Dong, Bin Int J Mol Sci Article CCCH is a zinc finger family with a typical CCCH-type motif which performs a variety of roles in plant growth and development and responses to environmental stressors. However, the information about this family has not been reported for Osmanthus fragrans. In this study, a total of 66 CCCH predicted genes were identified from the O. fragrans genome, the majority of which had multiple CCCH motifs. The 66 OfCCCHs were found to be unevenly distributed on 21 chromosomes and were clustered into nine groups based on their phylogenetic analysis. In each group, the gene structure and domain makeup were comparatively conserved. The expression profiles of the OfCCCH genes were examined in various tissues, the flower-opening processes, and under various abiotic stresses using transcriptome sequencing and qRT-PCR (quantitative real-time PCR). The results demonstrated the widespread expression of OfCCCHs in various tissues, the differential expression of 22 OfCCCHs during flower-opening stages, and the identification of 4, 5, and 13 OfCCCHs after ABA, salt, and drought stress treatment, respectively. Furthermore, characterization of the representative OfCCCHs (OfCCCH8, 23, 27, and 36) revealed that they were all localized in the nucleus and that the majority of them had transcriptional activation in the yeast system. Our research offers the first thorough examination of the OfCCCH family and lays the groundwork for future investigations regarding the functions of CCCH genes in O. fragrans. MDPI 2022-12-06 /pmc/articles/PMC9735588/ /pubmed/36499688 http://dx.doi.org/10.3390/ijms232315363 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Ye, Yong
Cao, Shanshan
Shen, Lixiao
Wang, Yiguang
Zhong, Shiwei
Yang, Liyuan
Xiao, Zheng
Fang, Qiu
Zhao, Hongbo
Dong, Bin
Comparative Transcriptome Analysis of CCCH Family in Roles of Flower Opening and Abiotic Stress in Osmanthus fragrans
title Comparative Transcriptome Analysis of CCCH Family in Roles of Flower Opening and Abiotic Stress in Osmanthus fragrans
title_full Comparative Transcriptome Analysis of CCCH Family in Roles of Flower Opening and Abiotic Stress in Osmanthus fragrans
title_fullStr Comparative Transcriptome Analysis of CCCH Family in Roles of Flower Opening and Abiotic Stress in Osmanthus fragrans
title_full_unstemmed Comparative Transcriptome Analysis of CCCH Family in Roles of Flower Opening and Abiotic Stress in Osmanthus fragrans
title_short Comparative Transcriptome Analysis of CCCH Family in Roles of Flower Opening and Abiotic Stress in Osmanthus fragrans
title_sort comparative transcriptome analysis of ccch family in roles of flower opening and abiotic stress in osmanthus fragrans
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9735588/
https://www.ncbi.nlm.nih.gov/pubmed/36499688
http://dx.doi.org/10.3390/ijms232315363
work_keys_str_mv AT yeyong comparativetranscriptomeanalysisofccchfamilyinrolesoffloweropeningandabioticstressinosmanthusfragrans
AT caoshanshan comparativetranscriptomeanalysisofccchfamilyinrolesoffloweropeningandabioticstressinosmanthusfragrans
AT shenlixiao comparativetranscriptomeanalysisofccchfamilyinrolesoffloweropeningandabioticstressinosmanthusfragrans
AT wangyiguang comparativetranscriptomeanalysisofccchfamilyinrolesoffloweropeningandabioticstressinosmanthusfragrans
AT zhongshiwei comparativetranscriptomeanalysisofccchfamilyinrolesoffloweropeningandabioticstressinosmanthusfragrans
AT yangliyuan comparativetranscriptomeanalysisofccchfamilyinrolesoffloweropeningandabioticstressinosmanthusfragrans
AT xiaozheng comparativetranscriptomeanalysisofccchfamilyinrolesoffloweropeningandabioticstressinosmanthusfragrans
AT fangqiu comparativetranscriptomeanalysisofccchfamilyinrolesoffloweropeningandabioticstressinosmanthusfragrans
AT zhaohongbo comparativetranscriptomeanalysisofccchfamilyinrolesoffloweropeningandabioticstressinosmanthusfragrans
AT dongbin comparativetranscriptomeanalysisofccchfamilyinrolesoffloweropeningandabioticstressinosmanthusfragrans