Cargando…

The Effect of the Stress-Signalling Mediator Triacontanol on Biochemical and Physiological Modifications in Dracocephalum forrestii Culture

Triacontanol (TRIA) has been reported to influence signal transduction in the crosstalk triggered by various stress factors. As a signal player, it is also known to affect many physiological processes, including enhancing the biosynthesis of secondary metabolites. Such knowledge can be used to direc...

Descripción completa

Detalles Bibliográficos
Autores principales: Weremczuk-Jeżyna, Izabela, Hnatuszko-Konka, Katarzyna, Lebelt, Liwia, Piotrowska, Dorota G., Grzegorczyk-Karolak, Izabela
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9735700/
https://www.ncbi.nlm.nih.gov/pubmed/36499476
http://dx.doi.org/10.3390/ijms232315147
_version_ 1784846835619201024
author Weremczuk-Jeżyna, Izabela
Hnatuszko-Konka, Katarzyna
Lebelt, Liwia
Piotrowska, Dorota G.
Grzegorczyk-Karolak, Izabela
author_facet Weremczuk-Jeżyna, Izabela
Hnatuszko-Konka, Katarzyna
Lebelt, Liwia
Piotrowska, Dorota G.
Grzegorczyk-Karolak, Izabela
author_sort Weremczuk-Jeżyna, Izabela
collection PubMed
description Triacontanol (TRIA) has been reported to influence signal transduction in the crosstalk triggered by various stress factors. As a signal player, it is also known to affect many physiological processes, including enhancing the biosynthesis of secondary metabolites. Such knowledge can be used to direct or boost the production of bioactive secondary compounds without stress induction. Therefore, the aim of this study is to evaluate the use of TRIA as a factor stimulating the growth and production of bioactive compounds in the shoot culture of Dracocephalum forrestii. TRIA was applied at three concentrations (2.5, 5, and 10 µM), alone or in combination with phytohormones (6-benzylaminopurine and indole-3-acetic acid). After five weeks, growth and physiochemical parameters (chlorophyll content, antioxidant enzyme activity, and phenolic acid level) were determined. The results indicate that TRIA application significantly increased shoot dry weight, chlorophyll content, antioxidant enzyme activities (superoxide dismutase, peroxidase, and catalase), and total polyphenol level; it also influenced the multiplication ratio in combination with growth regulators. The greatest antioxidant enzyme activity was observed for 5 µM TRIA in hormone-free medium, while the most significant secondary metabolite production was obtained for phytohormone-containing medium supplemented with 10 µM TRIA: total phenolic acid content (19.4 mg/g dry weight) was twice that of the control. Hence, the TRIA application appears to be a valuable biotechnology technique for modifying plant metabolite production.
format Online
Article
Text
id pubmed-9735700
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-97357002022-12-11 The Effect of the Stress-Signalling Mediator Triacontanol on Biochemical and Physiological Modifications in Dracocephalum forrestii Culture Weremczuk-Jeżyna, Izabela Hnatuszko-Konka, Katarzyna Lebelt, Liwia Piotrowska, Dorota G. Grzegorczyk-Karolak, Izabela Int J Mol Sci Article Triacontanol (TRIA) has been reported to influence signal transduction in the crosstalk triggered by various stress factors. As a signal player, it is also known to affect many physiological processes, including enhancing the biosynthesis of secondary metabolites. Such knowledge can be used to direct or boost the production of bioactive secondary compounds without stress induction. Therefore, the aim of this study is to evaluate the use of TRIA as a factor stimulating the growth and production of bioactive compounds in the shoot culture of Dracocephalum forrestii. TRIA was applied at three concentrations (2.5, 5, and 10 µM), alone or in combination with phytohormones (6-benzylaminopurine and indole-3-acetic acid). After five weeks, growth and physiochemical parameters (chlorophyll content, antioxidant enzyme activity, and phenolic acid level) were determined. The results indicate that TRIA application significantly increased shoot dry weight, chlorophyll content, antioxidant enzyme activities (superoxide dismutase, peroxidase, and catalase), and total polyphenol level; it also influenced the multiplication ratio in combination with growth regulators. The greatest antioxidant enzyme activity was observed for 5 µM TRIA in hormone-free medium, while the most significant secondary metabolite production was obtained for phytohormone-containing medium supplemented with 10 µM TRIA: total phenolic acid content (19.4 mg/g dry weight) was twice that of the control. Hence, the TRIA application appears to be a valuable biotechnology technique for modifying plant metabolite production. MDPI 2022-12-02 /pmc/articles/PMC9735700/ /pubmed/36499476 http://dx.doi.org/10.3390/ijms232315147 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Weremczuk-Jeżyna, Izabela
Hnatuszko-Konka, Katarzyna
Lebelt, Liwia
Piotrowska, Dorota G.
Grzegorczyk-Karolak, Izabela
The Effect of the Stress-Signalling Mediator Triacontanol on Biochemical and Physiological Modifications in Dracocephalum forrestii Culture
title The Effect of the Stress-Signalling Mediator Triacontanol on Biochemical and Physiological Modifications in Dracocephalum forrestii Culture
title_full The Effect of the Stress-Signalling Mediator Triacontanol on Biochemical and Physiological Modifications in Dracocephalum forrestii Culture
title_fullStr The Effect of the Stress-Signalling Mediator Triacontanol on Biochemical and Physiological Modifications in Dracocephalum forrestii Culture
title_full_unstemmed The Effect of the Stress-Signalling Mediator Triacontanol on Biochemical and Physiological Modifications in Dracocephalum forrestii Culture
title_short The Effect of the Stress-Signalling Mediator Triacontanol on Biochemical and Physiological Modifications in Dracocephalum forrestii Culture
title_sort effect of the stress-signalling mediator triacontanol on biochemical and physiological modifications in dracocephalum forrestii culture
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9735700/
https://www.ncbi.nlm.nih.gov/pubmed/36499476
http://dx.doi.org/10.3390/ijms232315147
work_keys_str_mv AT weremczukjezynaizabela theeffectofthestresssignallingmediatortriacontanolonbiochemicalandphysiologicalmodificationsindracocephalumforrestiiculture
AT hnatuszkokonkakatarzyna theeffectofthestresssignallingmediatortriacontanolonbiochemicalandphysiologicalmodificationsindracocephalumforrestiiculture
AT lebeltliwia theeffectofthestresssignallingmediatortriacontanolonbiochemicalandphysiologicalmodificationsindracocephalumforrestiiculture
AT piotrowskadorotag theeffectofthestresssignallingmediatortriacontanolonbiochemicalandphysiologicalmodificationsindracocephalumforrestiiculture
AT grzegorczykkarolakizabela theeffectofthestresssignallingmediatortriacontanolonbiochemicalandphysiologicalmodificationsindracocephalumforrestiiculture
AT weremczukjezynaizabela effectofthestresssignallingmediatortriacontanolonbiochemicalandphysiologicalmodificationsindracocephalumforrestiiculture
AT hnatuszkokonkakatarzyna effectofthestresssignallingmediatortriacontanolonbiochemicalandphysiologicalmodificationsindracocephalumforrestiiculture
AT lebeltliwia effectofthestresssignallingmediatortriacontanolonbiochemicalandphysiologicalmodificationsindracocephalumforrestiiculture
AT piotrowskadorotag effectofthestresssignallingmediatortriacontanolonbiochemicalandphysiologicalmodificationsindracocephalumforrestiiculture
AT grzegorczykkarolakizabela effectofthestresssignallingmediatortriacontanolonbiochemicalandphysiologicalmodificationsindracocephalumforrestiiculture