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The Role of Carbonic Anhydrase αCA4 in Photosynthetic Reactions in Arabidopsis thaliana Studied, Using the Cas9 and T-DNA Induced Mutations in Its Gene

An homozygous mutant line of Arabidopsis thaliana with a knocked out At4g20990 gene encoding thylakoid carbonic anhydrase αCA4 was created using a CRISPR/Cas9 genome editing system. The effects of the mutation were compared with those in two mutant lines obtained by the T-DNA insertion method. In αC...

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Autores principales: Rudenko, Natalia N., Permyakova, Natalya V., Ignatova, Lyudmila K., Nadeeva, Elena M., Zagorskaya, Alla A., Deineko, Elena V., Ivanov, Boris N.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9735932/
https://www.ncbi.nlm.nih.gov/pubmed/36501340
http://dx.doi.org/10.3390/plants11233303
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author Rudenko, Natalia N.
Permyakova, Natalya V.
Ignatova, Lyudmila K.
Nadeeva, Elena M.
Zagorskaya, Alla A.
Deineko, Elena V.
Ivanov, Boris N.
author_facet Rudenko, Natalia N.
Permyakova, Natalya V.
Ignatova, Lyudmila K.
Nadeeva, Elena M.
Zagorskaya, Alla A.
Deineko, Elena V.
Ivanov, Boris N.
author_sort Rudenko, Natalia N.
collection PubMed
description An homozygous mutant line of Arabidopsis thaliana with a knocked out At4g20990 gene encoding thylakoid carbonic anhydrase αCA4 was created using a CRISPR/Cas9 genome editing system. The effects of the mutation were compared with those in two mutant lines obtained by the T-DNA insertion method. In αCA4 knockouts of all three lines, non-photochemical quenching of chlorophyll a fluorescence was lower than in the wild type (WT) plants due to a decrease in its energy-dependent component. The αCA4 knockout also affected the level of expression of the genes encoding all proteins of the PSII light harvesting antennae, the genes encoding cytoplasmic and thylakoid CAs and the genes induced by plant immune signals. The production level of starch synthesis during the light period, as well as the level of its utilization during the darkness, were significantly higher in these mutants than in WT plants. These data confirm that the previously observed differences between insertional mutants and WT plants were not the result of the negative effects of T-DNA insertion transgenesis but the results of αCA4 gene knockout. Overall, the data indicate the involvement of αCA4 in the photosynthetic reactions in the thylakoid membrane, in particular in processes associated with the protection of higher plants’ photosynthetic apparatus from photoinhibition.
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spelling pubmed-97359322022-12-11 The Role of Carbonic Anhydrase αCA4 in Photosynthetic Reactions in Arabidopsis thaliana Studied, Using the Cas9 and T-DNA Induced Mutations in Its Gene Rudenko, Natalia N. Permyakova, Natalya V. Ignatova, Lyudmila K. Nadeeva, Elena M. Zagorskaya, Alla A. Deineko, Elena V. Ivanov, Boris N. Plants (Basel) Article An homozygous mutant line of Arabidopsis thaliana with a knocked out At4g20990 gene encoding thylakoid carbonic anhydrase αCA4 was created using a CRISPR/Cas9 genome editing system. The effects of the mutation were compared with those in two mutant lines obtained by the T-DNA insertion method. In αCA4 knockouts of all three lines, non-photochemical quenching of chlorophyll a fluorescence was lower than in the wild type (WT) plants due to a decrease in its energy-dependent component. The αCA4 knockout also affected the level of expression of the genes encoding all proteins of the PSII light harvesting antennae, the genes encoding cytoplasmic and thylakoid CAs and the genes induced by plant immune signals. The production level of starch synthesis during the light period, as well as the level of its utilization during the darkness, were significantly higher in these mutants than in WT plants. These data confirm that the previously observed differences between insertional mutants and WT plants were not the result of the negative effects of T-DNA insertion transgenesis but the results of αCA4 gene knockout. Overall, the data indicate the involvement of αCA4 in the photosynthetic reactions in the thylakoid membrane, in particular in processes associated with the protection of higher plants’ photosynthetic apparatus from photoinhibition. MDPI 2022-11-29 /pmc/articles/PMC9735932/ /pubmed/36501340 http://dx.doi.org/10.3390/plants11233303 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Rudenko, Natalia N.
Permyakova, Natalya V.
Ignatova, Lyudmila K.
Nadeeva, Elena M.
Zagorskaya, Alla A.
Deineko, Elena V.
Ivanov, Boris N.
The Role of Carbonic Anhydrase αCA4 in Photosynthetic Reactions in Arabidopsis thaliana Studied, Using the Cas9 and T-DNA Induced Mutations in Its Gene
title The Role of Carbonic Anhydrase αCA4 in Photosynthetic Reactions in Arabidopsis thaliana Studied, Using the Cas9 and T-DNA Induced Mutations in Its Gene
title_full The Role of Carbonic Anhydrase αCA4 in Photosynthetic Reactions in Arabidopsis thaliana Studied, Using the Cas9 and T-DNA Induced Mutations in Its Gene
title_fullStr The Role of Carbonic Anhydrase αCA4 in Photosynthetic Reactions in Arabidopsis thaliana Studied, Using the Cas9 and T-DNA Induced Mutations in Its Gene
title_full_unstemmed The Role of Carbonic Anhydrase αCA4 in Photosynthetic Reactions in Arabidopsis thaliana Studied, Using the Cas9 and T-DNA Induced Mutations in Its Gene
title_short The Role of Carbonic Anhydrase αCA4 in Photosynthetic Reactions in Arabidopsis thaliana Studied, Using the Cas9 and T-DNA Induced Mutations in Its Gene
title_sort role of carbonic anhydrase αca4 in photosynthetic reactions in arabidopsis thaliana studied, using the cas9 and t-dna induced mutations in its gene
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9735932/
https://www.ncbi.nlm.nih.gov/pubmed/36501340
http://dx.doi.org/10.3390/plants11233303
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