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Antibacterial Activity of Syzygium aromaticum (Clove) Bud Oil and Its Interaction with Imipenem in Controlling Wound Infections in Rats Caused by Methicillin-Resistant Staphylococcus aureus

Methicillin-resistant Staphylococcus aureus (MRSA) is one of the leading causes of infection worldwide. Clove oil’s ability to inhibit the growth of MRSA was studied through in vitro and in vivo studies. The phytochemical components of clove oil were determined through gas chromatography-mass spectr...

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Autores principales: Alanazi, Abdulaziz Khaleef, Alqasmi, Mohammed Hussein, Alrouji, Mohammed, Kuriri, Fahd A., Almuhanna, Yasir, Joseph, Babu, Asad, Mohammed
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9736006/
https://www.ncbi.nlm.nih.gov/pubmed/36500645
http://dx.doi.org/10.3390/molecules27238551
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author Alanazi, Abdulaziz Khaleef
Alqasmi, Mohammed Hussein
Alrouji, Mohammed
Kuriri, Fahd A.
Almuhanna, Yasir
Joseph, Babu
Asad, Mohammed
author_facet Alanazi, Abdulaziz Khaleef
Alqasmi, Mohammed Hussein
Alrouji, Mohammed
Kuriri, Fahd A.
Almuhanna, Yasir
Joseph, Babu
Asad, Mohammed
author_sort Alanazi, Abdulaziz Khaleef
collection PubMed
description Methicillin-resistant Staphylococcus aureus (MRSA) is one of the leading causes of infection worldwide. Clove oil’s ability to inhibit the growth of MRSA was studied through in vitro and in vivo studies. The phytochemical components of clove oil were determined through gas chromatography-mass spectrometry (GC-MS) analysis. The antibacterial effects of clove oil and its interaction with imipenem were determined by studying MIC, MBC, and FIC indices in vitro. The in vivo wound-healing effect of the clove oil and infection control were determined using excision wound model rats. The GC-MS analysis of clove oil revealed the presence of 16 volatile compounds. Clove oil showed a good antibacterial effect in vitro but no interaction was observed with imipenem. Clove bud oil alone or in combination with imipenem healed wounds faster and reduced the microbial load in wounds. The findings of this study confirmed the antibacterial activity of clove oil in vitro and in vivo and demonstrated its interaction with imipenem.
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spelling pubmed-97360062022-12-11 Antibacterial Activity of Syzygium aromaticum (Clove) Bud Oil and Its Interaction with Imipenem in Controlling Wound Infections in Rats Caused by Methicillin-Resistant Staphylococcus aureus Alanazi, Abdulaziz Khaleef Alqasmi, Mohammed Hussein Alrouji, Mohammed Kuriri, Fahd A. Almuhanna, Yasir Joseph, Babu Asad, Mohammed Molecules Article Methicillin-resistant Staphylococcus aureus (MRSA) is one of the leading causes of infection worldwide. Clove oil’s ability to inhibit the growth of MRSA was studied through in vitro and in vivo studies. The phytochemical components of clove oil were determined through gas chromatography-mass spectrometry (GC-MS) analysis. The antibacterial effects of clove oil and its interaction with imipenem were determined by studying MIC, MBC, and FIC indices in vitro. The in vivo wound-healing effect of the clove oil and infection control were determined using excision wound model rats. The GC-MS analysis of clove oil revealed the presence of 16 volatile compounds. Clove oil showed a good antibacterial effect in vitro but no interaction was observed with imipenem. Clove bud oil alone or in combination with imipenem healed wounds faster and reduced the microbial load in wounds. The findings of this study confirmed the antibacterial activity of clove oil in vitro and in vivo and demonstrated its interaction with imipenem. MDPI 2022-12-05 /pmc/articles/PMC9736006/ /pubmed/36500645 http://dx.doi.org/10.3390/molecules27238551 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Alanazi, Abdulaziz Khaleef
Alqasmi, Mohammed Hussein
Alrouji, Mohammed
Kuriri, Fahd A.
Almuhanna, Yasir
Joseph, Babu
Asad, Mohammed
Antibacterial Activity of Syzygium aromaticum (Clove) Bud Oil and Its Interaction with Imipenem in Controlling Wound Infections in Rats Caused by Methicillin-Resistant Staphylococcus aureus
title Antibacterial Activity of Syzygium aromaticum (Clove) Bud Oil and Its Interaction with Imipenem in Controlling Wound Infections in Rats Caused by Methicillin-Resistant Staphylococcus aureus
title_full Antibacterial Activity of Syzygium aromaticum (Clove) Bud Oil and Its Interaction with Imipenem in Controlling Wound Infections in Rats Caused by Methicillin-Resistant Staphylococcus aureus
title_fullStr Antibacterial Activity of Syzygium aromaticum (Clove) Bud Oil and Its Interaction with Imipenem in Controlling Wound Infections in Rats Caused by Methicillin-Resistant Staphylococcus aureus
title_full_unstemmed Antibacterial Activity of Syzygium aromaticum (Clove) Bud Oil and Its Interaction with Imipenem in Controlling Wound Infections in Rats Caused by Methicillin-Resistant Staphylococcus aureus
title_short Antibacterial Activity of Syzygium aromaticum (Clove) Bud Oil and Its Interaction with Imipenem in Controlling Wound Infections in Rats Caused by Methicillin-Resistant Staphylococcus aureus
title_sort antibacterial activity of syzygium aromaticum (clove) bud oil and its interaction with imipenem in controlling wound infections in rats caused by methicillin-resistant staphylococcus aureus
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9736006/
https://www.ncbi.nlm.nih.gov/pubmed/36500645
http://dx.doi.org/10.3390/molecules27238551
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