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Acarbose Protects Glucolipotoxicity-Induced Diabetic Nephropathy by Inhibiting Ras Expression in High-Fat Diet-Fed db/db Mice

Diabetic nephropathy (DN) exacerbates renal tissue damage and is a major cause of end-stage renal disease. Reactive oxygen species play a vital role in hyperglycemia-induced renal injury. This study examined whether the oral hypoglycemic drug acarbose (Ab) could attenuate the progression of DN in ty...

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Autores principales: Hung, Tung-Wei, Yu, Meng-Hsun, Yang, Tsung-Yuan, Yang, Mon-Yuan, Chen, Jia-Yu, Chan, Kuei-Chuan, Wang, Chau-Jong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9736061/
https://www.ncbi.nlm.nih.gov/pubmed/36499639
http://dx.doi.org/10.3390/ijms232315312
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author Hung, Tung-Wei
Yu, Meng-Hsun
Yang, Tsung-Yuan
Yang, Mon-Yuan
Chen, Jia-Yu
Chan, Kuei-Chuan
Wang, Chau-Jong
author_facet Hung, Tung-Wei
Yu, Meng-Hsun
Yang, Tsung-Yuan
Yang, Mon-Yuan
Chen, Jia-Yu
Chan, Kuei-Chuan
Wang, Chau-Jong
author_sort Hung, Tung-Wei
collection PubMed
description Diabetic nephropathy (DN) exacerbates renal tissue damage and is a major cause of end-stage renal disease. Reactive oxygen species play a vital role in hyperglycemia-induced renal injury. This study examined whether the oral hypoglycemic drug acarbose (Ab) could attenuate the progression of DN in type 2 diabetes mellitus mice. In this study, 50 mg/kg body weight of Ab was administered to high-fat diet (HFD)-fed db/db mice. Their body weight was recorded every week, and the serum glucose concentration was monitored every 2 weeks. Following their euthanasia, the kidneys of mice were analyzed through hematoxylin and eosin, periodic acid Schiff, Masson’s trichrome, and immunohistochemistry (IHC) staining. The results revealed that Ab stabilized the plasma glucose and indirectly improved the insulin sensitivity and renal functional biomarkers in diabetic mice. In addition, diabetes-induced glomerular hypertrophy, the saccharide accumulation, and formation of collagen fiber were reduced in diabetic mice receiving Ab. Although the dosages of Ab cannot decrease the blood sugar in db/db mice, our results indicate that Ab alleviates glucolipotoxicity-induced DN by inhibiting kidney fibrosis-related proteins through the Ras/ERK pathway.
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spelling pubmed-97360612022-12-11 Acarbose Protects Glucolipotoxicity-Induced Diabetic Nephropathy by Inhibiting Ras Expression in High-Fat Diet-Fed db/db Mice Hung, Tung-Wei Yu, Meng-Hsun Yang, Tsung-Yuan Yang, Mon-Yuan Chen, Jia-Yu Chan, Kuei-Chuan Wang, Chau-Jong Int J Mol Sci Article Diabetic nephropathy (DN) exacerbates renal tissue damage and is a major cause of end-stage renal disease. Reactive oxygen species play a vital role in hyperglycemia-induced renal injury. This study examined whether the oral hypoglycemic drug acarbose (Ab) could attenuate the progression of DN in type 2 diabetes mellitus mice. In this study, 50 mg/kg body weight of Ab was administered to high-fat diet (HFD)-fed db/db mice. Their body weight was recorded every week, and the serum glucose concentration was monitored every 2 weeks. Following their euthanasia, the kidneys of mice were analyzed through hematoxylin and eosin, periodic acid Schiff, Masson’s trichrome, and immunohistochemistry (IHC) staining. The results revealed that Ab stabilized the plasma glucose and indirectly improved the insulin sensitivity and renal functional biomarkers in diabetic mice. In addition, diabetes-induced glomerular hypertrophy, the saccharide accumulation, and formation of collagen fiber were reduced in diabetic mice receiving Ab. Although the dosages of Ab cannot decrease the blood sugar in db/db mice, our results indicate that Ab alleviates glucolipotoxicity-induced DN by inhibiting kidney fibrosis-related proteins through the Ras/ERK pathway. MDPI 2022-12-05 /pmc/articles/PMC9736061/ /pubmed/36499639 http://dx.doi.org/10.3390/ijms232315312 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Hung, Tung-Wei
Yu, Meng-Hsun
Yang, Tsung-Yuan
Yang, Mon-Yuan
Chen, Jia-Yu
Chan, Kuei-Chuan
Wang, Chau-Jong
Acarbose Protects Glucolipotoxicity-Induced Diabetic Nephropathy by Inhibiting Ras Expression in High-Fat Diet-Fed db/db Mice
title Acarbose Protects Glucolipotoxicity-Induced Diabetic Nephropathy by Inhibiting Ras Expression in High-Fat Diet-Fed db/db Mice
title_full Acarbose Protects Glucolipotoxicity-Induced Diabetic Nephropathy by Inhibiting Ras Expression in High-Fat Diet-Fed db/db Mice
title_fullStr Acarbose Protects Glucolipotoxicity-Induced Diabetic Nephropathy by Inhibiting Ras Expression in High-Fat Diet-Fed db/db Mice
title_full_unstemmed Acarbose Protects Glucolipotoxicity-Induced Diabetic Nephropathy by Inhibiting Ras Expression in High-Fat Diet-Fed db/db Mice
title_short Acarbose Protects Glucolipotoxicity-Induced Diabetic Nephropathy by Inhibiting Ras Expression in High-Fat Diet-Fed db/db Mice
title_sort acarbose protects glucolipotoxicity-induced diabetic nephropathy by inhibiting ras expression in high-fat diet-fed db/db mice
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9736061/
https://www.ncbi.nlm.nih.gov/pubmed/36499639
http://dx.doi.org/10.3390/ijms232315312
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