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Antimicrobial Activity of Smilax china L. Root Extracts against the Acne-Causing Bacterium, Cutibacterium acnes, and Its Active Compounds
The root of Smilax china L. is used in traditional Korean medicine. We found that the Smilax china L. root extract has strong antimicrobial activity against two Cutibacterium acnes strains (KCTC 3314 and KCTC 3320). The aim of this study was to identify the beneficial properties of Smilax china L. e...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9736125/ https://www.ncbi.nlm.nih.gov/pubmed/36500424 http://dx.doi.org/10.3390/molecules27238331 |
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author | Joo, Ji-Hae Han, Min-Hui Kim, Ja-I Kim, Jong-Eun Jung, Kyung-Hwan Oh, Han Sun Chung, Young Soo An, Hyun Jin Lee, Jae Duk Moon, Gi-Seong Lee, Hyang-Yeol |
author_facet | Joo, Ji-Hae Han, Min-Hui Kim, Ja-I Kim, Jong-Eun Jung, Kyung-Hwan Oh, Han Sun Chung, Young Soo An, Hyun Jin Lee, Jae Duk Moon, Gi-Seong Lee, Hyang-Yeol |
author_sort | Joo, Ji-Hae |
collection | PubMed |
description | The root of Smilax china L. is used in traditional Korean medicine. We found that the Smilax china L. root extract has strong antimicrobial activity against two Cutibacterium acnes strains (KCTC 3314 and KCTC 3320). The aim of this study was to identify the beneficial properties of Smilax china L. extracts for their potential use as active ingredients in cosmetics for the treatment of human skin acne. The high-performance liquid chromatography (HPLC) and liquid chromatography-hybrid quadrupole time-of-flight mass spectrometry (LC/QTOF/MS) methods were used to obtain the profile of secondary metabolites from the ethyl acetate-soluble fraction of the crude extract. Agar diffusion and resazurin-based broth microdilution assays were used to evaluate antimicrobial activity and minimum inhibitory concentrations (MIC), respectively. Among the 24 metabolites, quercetin, resveratrol, and oxyresveratrol were the most potent compounds against Cutibacterium acnes. Minimum inhibitory concentrations of quercetin, resveratrol, and oxyresveratrol were 31.25, 125, and 250 μg/mL, respectively. |
format | Online Article Text |
id | pubmed-9736125 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-97361252022-12-11 Antimicrobial Activity of Smilax china L. Root Extracts against the Acne-Causing Bacterium, Cutibacterium acnes, and Its Active Compounds Joo, Ji-Hae Han, Min-Hui Kim, Ja-I Kim, Jong-Eun Jung, Kyung-Hwan Oh, Han Sun Chung, Young Soo An, Hyun Jin Lee, Jae Duk Moon, Gi-Seong Lee, Hyang-Yeol Molecules Article The root of Smilax china L. is used in traditional Korean medicine. We found that the Smilax china L. root extract has strong antimicrobial activity against two Cutibacterium acnes strains (KCTC 3314 and KCTC 3320). The aim of this study was to identify the beneficial properties of Smilax china L. extracts for their potential use as active ingredients in cosmetics for the treatment of human skin acne. The high-performance liquid chromatography (HPLC) and liquid chromatography-hybrid quadrupole time-of-flight mass spectrometry (LC/QTOF/MS) methods were used to obtain the profile of secondary metabolites from the ethyl acetate-soluble fraction of the crude extract. Agar diffusion and resazurin-based broth microdilution assays were used to evaluate antimicrobial activity and minimum inhibitory concentrations (MIC), respectively. Among the 24 metabolites, quercetin, resveratrol, and oxyresveratrol were the most potent compounds against Cutibacterium acnes. Minimum inhibitory concentrations of quercetin, resveratrol, and oxyresveratrol were 31.25, 125, and 250 μg/mL, respectively. MDPI 2022-11-29 /pmc/articles/PMC9736125/ /pubmed/36500424 http://dx.doi.org/10.3390/molecules27238331 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Joo, Ji-Hae Han, Min-Hui Kim, Ja-I Kim, Jong-Eun Jung, Kyung-Hwan Oh, Han Sun Chung, Young Soo An, Hyun Jin Lee, Jae Duk Moon, Gi-Seong Lee, Hyang-Yeol Antimicrobial Activity of Smilax china L. Root Extracts against the Acne-Causing Bacterium, Cutibacterium acnes, and Its Active Compounds |
title | Antimicrobial Activity of Smilax china L. Root Extracts against the Acne-Causing Bacterium, Cutibacterium acnes, and Its Active Compounds |
title_full | Antimicrobial Activity of Smilax china L. Root Extracts against the Acne-Causing Bacterium, Cutibacterium acnes, and Its Active Compounds |
title_fullStr | Antimicrobial Activity of Smilax china L. Root Extracts against the Acne-Causing Bacterium, Cutibacterium acnes, and Its Active Compounds |
title_full_unstemmed | Antimicrobial Activity of Smilax china L. Root Extracts against the Acne-Causing Bacterium, Cutibacterium acnes, and Its Active Compounds |
title_short | Antimicrobial Activity of Smilax china L. Root Extracts against the Acne-Causing Bacterium, Cutibacterium acnes, and Its Active Compounds |
title_sort | antimicrobial activity of smilax china l. root extracts against the acne-causing bacterium, cutibacterium acnes, and its active compounds |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9736125/ https://www.ncbi.nlm.nih.gov/pubmed/36500424 http://dx.doi.org/10.3390/molecules27238331 |
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