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Visualizing Intramolecular Dynamics of Membrane Proteins

Membrane proteins play important roles in biological functions, with accompanying allosteric structure changes. Understanding intramolecular dynamics helps elucidate catalytic mechanisms and develop new drugs. In contrast to the various technologies for structural analysis, methods for analyzing int...

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Autores principales: Ohkubo, Tatsunari, Shiina, Takaaki, Kawaguchi, Kayoko, Sasaki, Daisuke, Inamasu, Rena, Yang, Yue, Li, Zhuoqi, Taninaka, Keizaburo, Sakaguchi, Masaki, Fujimura, Shoko, Sekiguchi, Hiroshi, Kuramochi, Masahiro, Arai, Tatsuya, Tsuda, Sakae, Sasaki, Yuji C., Mio, Kazuhiro
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9736139/
https://www.ncbi.nlm.nih.gov/pubmed/36498865
http://dx.doi.org/10.3390/ijms232314539
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author Ohkubo, Tatsunari
Shiina, Takaaki
Kawaguchi, Kayoko
Sasaki, Daisuke
Inamasu, Rena
Yang, Yue
Li, Zhuoqi
Taninaka, Keizaburo
Sakaguchi, Masaki
Fujimura, Shoko
Sekiguchi, Hiroshi
Kuramochi, Masahiro
Arai, Tatsuya
Tsuda, Sakae
Sasaki, Yuji C.
Mio, Kazuhiro
author_facet Ohkubo, Tatsunari
Shiina, Takaaki
Kawaguchi, Kayoko
Sasaki, Daisuke
Inamasu, Rena
Yang, Yue
Li, Zhuoqi
Taninaka, Keizaburo
Sakaguchi, Masaki
Fujimura, Shoko
Sekiguchi, Hiroshi
Kuramochi, Masahiro
Arai, Tatsuya
Tsuda, Sakae
Sasaki, Yuji C.
Mio, Kazuhiro
author_sort Ohkubo, Tatsunari
collection PubMed
description Membrane proteins play important roles in biological functions, with accompanying allosteric structure changes. Understanding intramolecular dynamics helps elucidate catalytic mechanisms and develop new drugs. In contrast to the various technologies for structural analysis, methods for analyzing intramolecular dynamics are limited. Single-molecule measurements using optical microscopy have been widely used for kinetic analysis. Recently, improvements in detectors and image analysis technology have made it possible to use single-molecule determination methods using X-rays and electron beams, such as diffracted X-ray tracking (DXT), X-ray free electron laser (XFEL) imaging, and cryo-electron microscopy (cryo-EM). High-speed atomic force microscopy (HS-AFM) is a scanning probe microscope that can capture the structural dynamics of biomolecules in real time at the single-molecule level. Time-resolved techniques also facilitate an understanding of real-time intramolecular processes during chemical reactions. In this review, recent advances in membrane protein dynamics visualization techniques were presented.
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spelling pubmed-97361392022-12-11 Visualizing Intramolecular Dynamics of Membrane Proteins Ohkubo, Tatsunari Shiina, Takaaki Kawaguchi, Kayoko Sasaki, Daisuke Inamasu, Rena Yang, Yue Li, Zhuoqi Taninaka, Keizaburo Sakaguchi, Masaki Fujimura, Shoko Sekiguchi, Hiroshi Kuramochi, Masahiro Arai, Tatsuya Tsuda, Sakae Sasaki, Yuji C. Mio, Kazuhiro Int J Mol Sci Review Membrane proteins play important roles in biological functions, with accompanying allosteric structure changes. Understanding intramolecular dynamics helps elucidate catalytic mechanisms and develop new drugs. In contrast to the various technologies for structural analysis, methods for analyzing intramolecular dynamics are limited. Single-molecule measurements using optical microscopy have been widely used for kinetic analysis. Recently, improvements in detectors and image analysis technology have made it possible to use single-molecule determination methods using X-rays and electron beams, such as diffracted X-ray tracking (DXT), X-ray free electron laser (XFEL) imaging, and cryo-electron microscopy (cryo-EM). High-speed atomic force microscopy (HS-AFM) is a scanning probe microscope that can capture the structural dynamics of biomolecules in real time at the single-molecule level. Time-resolved techniques also facilitate an understanding of real-time intramolecular processes during chemical reactions. In this review, recent advances in membrane protein dynamics visualization techniques were presented. MDPI 2022-11-22 /pmc/articles/PMC9736139/ /pubmed/36498865 http://dx.doi.org/10.3390/ijms232314539 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Review
Ohkubo, Tatsunari
Shiina, Takaaki
Kawaguchi, Kayoko
Sasaki, Daisuke
Inamasu, Rena
Yang, Yue
Li, Zhuoqi
Taninaka, Keizaburo
Sakaguchi, Masaki
Fujimura, Shoko
Sekiguchi, Hiroshi
Kuramochi, Masahiro
Arai, Tatsuya
Tsuda, Sakae
Sasaki, Yuji C.
Mio, Kazuhiro
Visualizing Intramolecular Dynamics of Membrane Proteins
title Visualizing Intramolecular Dynamics of Membrane Proteins
title_full Visualizing Intramolecular Dynamics of Membrane Proteins
title_fullStr Visualizing Intramolecular Dynamics of Membrane Proteins
title_full_unstemmed Visualizing Intramolecular Dynamics of Membrane Proteins
title_short Visualizing Intramolecular Dynamics of Membrane Proteins
title_sort visualizing intramolecular dynamics of membrane proteins
topic Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9736139/
https://www.ncbi.nlm.nih.gov/pubmed/36498865
http://dx.doi.org/10.3390/ijms232314539
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