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Ex Vivo Evaluation of Ethosomes and Transethosomes Applied on Human Skin: A Comparative Study

In this study, the transdermal fate of vesicular nanosystems was investigated. Particularly, ethosomes based on phosphatidylcholine 0.9% w/w and transethosomes based on phosphatidylcholine 0.9 or 2.7% w/w plus polysorbate 80 0.3% w/w as an edge activator were prepared and characterized. The vesicle...

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Autores principales: Esposito, Elisabetta, Calderan, Laura, Galvan, Andrea, Cappellozza, Enrica, Drechsler, Markus, Mariani, Paolo, Pepe, Alessia, Sguizzato, Maddalena, Vigato, Enrico, Dalla Pozza, Edoardo, Malatesta, Manuela
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
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Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9736248/
https://www.ncbi.nlm.nih.gov/pubmed/36499432
http://dx.doi.org/10.3390/ijms232315112
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author Esposito, Elisabetta
Calderan, Laura
Galvan, Andrea
Cappellozza, Enrica
Drechsler, Markus
Mariani, Paolo
Pepe, Alessia
Sguizzato, Maddalena
Vigato, Enrico
Dalla Pozza, Edoardo
Malatesta, Manuela
author_facet Esposito, Elisabetta
Calderan, Laura
Galvan, Andrea
Cappellozza, Enrica
Drechsler, Markus
Mariani, Paolo
Pepe, Alessia
Sguizzato, Maddalena
Vigato, Enrico
Dalla Pozza, Edoardo
Malatesta, Manuela
author_sort Esposito, Elisabetta
collection PubMed
description In this study, the transdermal fate of vesicular nanosystems was investigated. Particularly, ethosomes based on phosphatidylcholine 0.9% w/w and transethosomes based on phosphatidylcholine 0.9 or 2.7% w/w plus polysorbate 80 0.3% w/w as an edge activator were prepared and characterized. The vesicle mean size, morphology and deformability were influenced by both phosphatidylcholine and polysorbate 80. Indeed, the mean diameters of ethosome were around 200 nm, while transethosome’s mean diameters were 146 or 350 nm in the case of phosphatidylcholine 0.9 or 2.7%, w/w, respectively. The highest deformability was achieved by transethosomes based on phosphatidylcholine 0.9%, w/w. The three types of vesicular nanosystems were applied on explanted human skin maintained in a bioreactor. Transmission electron microscopy demonstrated that all vesicles were able to enter the skin, keeping their structural integrity. Notably, the vesicle penetration capability was influenced by their physical-chemical features. Indeed, ethosomes reached keratinocytes and even the dermis, phosphatidylcholine 0.9% transethosomes were found in keratinocytes and phosphatidylcholine 2.7% transethosomes were found only in corneocytes of the outer layer. These findings open interesting perspectives for a differentiated application of these vesicles for transdermal drug delivery as a function of the cutaneous pathology to be addressed.
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spelling pubmed-97362482022-12-11 Ex Vivo Evaluation of Ethosomes and Transethosomes Applied on Human Skin: A Comparative Study Esposito, Elisabetta Calderan, Laura Galvan, Andrea Cappellozza, Enrica Drechsler, Markus Mariani, Paolo Pepe, Alessia Sguizzato, Maddalena Vigato, Enrico Dalla Pozza, Edoardo Malatesta, Manuela Int J Mol Sci Article In this study, the transdermal fate of vesicular nanosystems was investigated. Particularly, ethosomes based on phosphatidylcholine 0.9% w/w and transethosomes based on phosphatidylcholine 0.9 or 2.7% w/w plus polysorbate 80 0.3% w/w as an edge activator were prepared and characterized. The vesicle mean size, morphology and deformability were influenced by both phosphatidylcholine and polysorbate 80. Indeed, the mean diameters of ethosome were around 200 nm, while transethosome’s mean diameters were 146 or 350 nm in the case of phosphatidylcholine 0.9 or 2.7%, w/w, respectively. The highest deformability was achieved by transethosomes based on phosphatidylcholine 0.9%, w/w. The three types of vesicular nanosystems were applied on explanted human skin maintained in a bioreactor. Transmission electron microscopy demonstrated that all vesicles were able to enter the skin, keeping their structural integrity. Notably, the vesicle penetration capability was influenced by their physical-chemical features. Indeed, ethosomes reached keratinocytes and even the dermis, phosphatidylcholine 0.9% transethosomes were found in keratinocytes and phosphatidylcholine 2.7% transethosomes were found only in corneocytes of the outer layer. These findings open interesting perspectives for a differentiated application of these vesicles for transdermal drug delivery as a function of the cutaneous pathology to be addressed. MDPI 2022-12-01 /pmc/articles/PMC9736248/ /pubmed/36499432 http://dx.doi.org/10.3390/ijms232315112 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Esposito, Elisabetta
Calderan, Laura
Galvan, Andrea
Cappellozza, Enrica
Drechsler, Markus
Mariani, Paolo
Pepe, Alessia
Sguizzato, Maddalena
Vigato, Enrico
Dalla Pozza, Edoardo
Malatesta, Manuela
Ex Vivo Evaluation of Ethosomes and Transethosomes Applied on Human Skin: A Comparative Study
title Ex Vivo Evaluation of Ethosomes and Transethosomes Applied on Human Skin: A Comparative Study
title_full Ex Vivo Evaluation of Ethosomes and Transethosomes Applied on Human Skin: A Comparative Study
title_fullStr Ex Vivo Evaluation of Ethosomes and Transethosomes Applied on Human Skin: A Comparative Study
title_full_unstemmed Ex Vivo Evaluation of Ethosomes and Transethosomes Applied on Human Skin: A Comparative Study
title_short Ex Vivo Evaluation of Ethosomes and Transethosomes Applied on Human Skin: A Comparative Study
title_sort ex vivo evaluation of ethosomes and transethosomes applied on human skin: a comparative study
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9736248/
https://www.ncbi.nlm.nih.gov/pubmed/36499432
http://dx.doi.org/10.3390/ijms232315112
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