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Red Cell Microparticles Suppress Hematoma Growth Following Intracerebral Hemorrhage in Chronic Nicotine-Exposed Rats
Spontaneous intracerebral hemorrhage (sICH) is a disabling stroke sub-type, and tobacco use is a prominent risk factor for sICH. We showed that chronic nicotine exposure enhances bleeding post-sICH. Reduction of hematoma growth is a promising effective therapy for sICH in smoking subjects. Red-blood...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9736308/ https://www.ncbi.nlm.nih.gov/pubmed/36499494 http://dx.doi.org/10.3390/ijms232315167 |
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author | Rehni, Ashish K. Cho, Sunjoo Zhang, Zhexuan Khushal, Priyanka Raval, Ami P. Koch, Sebastian Perez-Pinzon, Miguel A. Zhao, Weizhao Jy, Wenche Dave, Kunjan R. |
author_facet | Rehni, Ashish K. Cho, Sunjoo Zhang, Zhexuan Khushal, Priyanka Raval, Ami P. Koch, Sebastian Perez-Pinzon, Miguel A. Zhao, Weizhao Jy, Wenche Dave, Kunjan R. |
author_sort | Rehni, Ashish K. |
collection | PubMed |
description | Spontaneous intracerebral hemorrhage (sICH) is a disabling stroke sub-type, and tobacco use is a prominent risk factor for sICH. We showed that chronic nicotine exposure enhances bleeding post-sICH. Reduction of hematoma growth is a promising effective therapy for sICH in smoking subjects. Red-blood-cell-derived microparticles (RMPs) are hemostatic agents that limit hematoma expansion following sICH in naïve rats. Considering the importance of testing the efficacy of experimental drugs in animal models with a risk factor for a disease, we tested RMP efficacy and the therapeutic time window in limiting hematoma growth post-sICH in rats exposed to nicotine. Young rats were chronically treated with nicotine using osmotic pumps. sICH was induced in rats using an injection of collagenase in the right striatum. Vehicle/RMPs were administered intravenously. Hematoma volume and neurological impairment were quantified ≈24 h after sICH. Hematoma volumes in male and female nicotine-exposed rats that were treated with RMPs at 2 h post-sICH were significantly lower by 26 and 31% when compared to their respective control groups. RMP therapy was able to limit hematoma volume when administered up to 4.5 h post-sICH in animals of both sexes. Therefore, RMPs may limit hematoma growth in sICH patients exposed to tobacco use. |
format | Online Article Text |
id | pubmed-9736308 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-97363082022-12-11 Red Cell Microparticles Suppress Hematoma Growth Following Intracerebral Hemorrhage in Chronic Nicotine-Exposed Rats Rehni, Ashish K. Cho, Sunjoo Zhang, Zhexuan Khushal, Priyanka Raval, Ami P. Koch, Sebastian Perez-Pinzon, Miguel A. Zhao, Weizhao Jy, Wenche Dave, Kunjan R. Int J Mol Sci Article Spontaneous intracerebral hemorrhage (sICH) is a disabling stroke sub-type, and tobacco use is a prominent risk factor for sICH. We showed that chronic nicotine exposure enhances bleeding post-sICH. Reduction of hematoma growth is a promising effective therapy for sICH in smoking subjects. Red-blood-cell-derived microparticles (RMPs) are hemostatic agents that limit hematoma expansion following sICH in naïve rats. Considering the importance of testing the efficacy of experimental drugs in animal models with a risk factor for a disease, we tested RMP efficacy and the therapeutic time window in limiting hematoma growth post-sICH in rats exposed to nicotine. Young rats were chronically treated with nicotine using osmotic pumps. sICH was induced in rats using an injection of collagenase in the right striatum. Vehicle/RMPs were administered intravenously. Hematoma volume and neurological impairment were quantified ≈24 h after sICH. Hematoma volumes in male and female nicotine-exposed rats that were treated with RMPs at 2 h post-sICH were significantly lower by 26 and 31% when compared to their respective control groups. RMP therapy was able to limit hematoma volume when administered up to 4.5 h post-sICH in animals of both sexes. Therefore, RMPs may limit hematoma growth in sICH patients exposed to tobacco use. MDPI 2022-12-02 /pmc/articles/PMC9736308/ /pubmed/36499494 http://dx.doi.org/10.3390/ijms232315167 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Rehni, Ashish K. Cho, Sunjoo Zhang, Zhexuan Khushal, Priyanka Raval, Ami P. Koch, Sebastian Perez-Pinzon, Miguel A. Zhao, Weizhao Jy, Wenche Dave, Kunjan R. Red Cell Microparticles Suppress Hematoma Growth Following Intracerebral Hemorrhage in Chronic Nicotine-Exposed Rats |
title | Red Cell Microparticles Suppress Hematoma Growth Following Intracerebral Hemorrhage in Chronic Nicotine-Exposed Rats |
title_full | Red Cell Microparticles Suppress Hematoma Growth Following Intracerebral Hemorrhage in Chronic Nicotine-Exposed Rats |
title_fullStr | Red Cell Microparticles Suppress Hematoma Growth Following Intracerebral Hemorrhage in Chronic Nicotine-Exposed Rats |
title_full_unstemmed | Red Cell Microparticles Suppress Hematoma Growth Following Intracerebral Hemorrhage in Chronic Nicotine-Exposed Rats |
title_short | Red Cell Microparticles Suppress Hematoma Growth Following Intracerebral Hemorrhage in Chronic Nicotine-Exposed Rats |
title_sort | red cell microparticles suppress hematoma growth following intracerebral hemorrhage in chronic nicotine-exposed rats |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9736308/ https://www.ncbi.nlm.nih.gov/pubmed/36499494 http://dx.doi.org/10.3390/ijms232315167 |
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