Cargando…

Therapeutic Effects of Acer palmatum Thumb. Leaf Extract (KIOM-2015E) on Benzalkonium Chloride-Induced Dry Eye in a Mouse Model

We determined the effects of two extracts from Acer palmatum Thumb. leaves (hot water extract KIOM-2015EW and 25% ethanol extract KIOM-2015EE) in a benzalkonium chloride (BAC)-induced dry eye mouse model. Dry eye was induced by 0.2% BAC for 2 weeks, followed by treatment three times (eye drop) or on...

Descripción completa

Detalles Bibliográficos
Autores principales: Yim, Nam-Hui, Park, Eunhee, Cho, Won-Kyung, Kim, Yeoun-Hee, Ma, Jin Yeul
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9736398/
https://www.ncbi.nlm.nih.gov/pubmed/36499298
http://dx.doi.org/10.3390/ijms232314964
_version_ 1784847016887582720
author Yim, Nam-Hui
Park, Eunhee
Cho, Won-Kyung
Kim, Yeoun-Hee
Ma, Jin Yeul
author_facet Yim, Nam-Hui
Park, Eunhee
Cho, Won-Kyung
Kim, Yeoun-Hee
Ma, Jin Yeul
author_sort Yim, Nam-Hui
collection PubMed
description We determined the effects of two extracts from Acer palmatum Thumb. leaves (hot water extract KIOM-2015EW and 25% ethanol extract KIOM-2015EE) in a benzalkonium chloride (BAC)-induced dry eye mouse model. Dry eye was induced by 0.2% BAC for 2 weeks, followed by treatment three times (eye drop) or once (oral administration) daily with KIOM-2015E for 2 weeks. Treatment with both KIOM-2015EE and KIOM-2015EW resulted in a marked increase in tear volume production for the 4 days of treatment. The Lissamine Green staining score, TUNEL-positive cells, and inflammatory index were significantly decreased after 2 weeks. Topical KIOM-2015EE administration exhibited a greater improvement in decreasing the ocular surface staining scores, inflammation, dead cells, and increasing tear production in a dose-dependent manner compared with the other groups. Furthermore, KIOM-2015E significantly reduced the phosphorylation of NF-κB, which was activated in the BAC-treated cornea. Topical administration was much more effective than oral administration for KIOM-2015E and KIOM-2015EE was more effective than KIOM-2015EW. Application of KIOM-2015E resulted in clinical improvement, inhibited the inflammatory response, and alleviated signs of dry eye. These results indicate that KIOM-2015E has potential as a therapeutic agent for the clinical treatment of dry eye.
format Online
Article
Text
id pubmed-9736398
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-97363982022-12-11 Therapeutic Effects of Acer palmatum Thumb. Leaf Extract (KIOM-2015E) on Benzalkonium Chloride-Induced Dry Eye in a Mouse Model Yim, Nam-Hui Park, Eunhee Cho, Won-Kyung Kim, Yeoun-Hee Ma, Jin Yeul Int J Mol Sci Article We determined the effects of two extracts from Acer palmatum Thumb. leaves (hot water extract KIOM-2015EW and 25% ethanol extract KIOM-2015EE) in a benzalkonium chloride (BAC)-induced dry eye mouse model. Dry eye was induced by 0.2% BAC for 2 weeks, followed by treatment three times (eye drop) or once (oral administration) daily with KIOM-2015E for 2 weeks. Treatment with both KIOM-2015EE and KIOM-2015EW resulted in a marked increase in tear volume production for the 4 days of treatment. The Lissamine Green staining score, TUNEL-positive cells, and inflammatory index were significantly decreased after 2 weeks. Topical KIOM-2015EE administration exhibited a greater improvement in decreasing the ocular surface staining scores, inflammation, dead cells, and increasing tear production in a dose-dependent manner compared with the other groups. Furthermore, KIOM-2015E significantly reduced the phosphorylation of NF-κB, which was activated in the BAC-treated cornea. Topical administration was much more effective than oral administration for KIOM-2015E and KIOM-2015EE was more effective than KIOM-2015EW. Application of KIOM-2015E resulted in clinical improvement, inhibited the inflammatory response, and alleviated signs of dry eye. These results indicate that KIOM-2015E has potential as a therapeutic agent for the clinical treatment of dry eye. MDPI 2022-11-29 /pmc/articles/PMC9736398/ /pubmed/36499298 http://dx.doi.org/10.3390/ijms232314964 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Yim, Nam-Hui
Park, Eunhee
Cho, Won-Kyung
Kim, Yeoun-Hee
Ma, Jin Yeul
Therapeutic Effects of Acer palmatum Thumb. Leaf Extract (KIOM-2015E) on Benzalkonium Chloride-Induced Dry Eye in a Mouse Model
title Therapeutic Effects of Acer palmatum Thumb. Leaf Extract (KIOM-2015E) on Benzalkonium Chloride-Induced Dry Eye in a Mouse Model
title_full Therapeutic Effects of Acer palmatum Thumb. Leaf Extract (KIOM-2015E) on Benzalkonium Chloride-Induced Dry Eye in a Mouse Model
title_fullStr Therapeutic Effects of Acer palmatum Thumb. Leaf Extract (KIOM-2015E) on Benzalkonium Chloride-Induced Dry Eye in a Mouse Model
title_full_unstemmed Therapeutic Effects of Acer palmatum Thumb. Leaf Extract (KIOM-2015E) on Benzalkonium Chloride-Induced Dry Eye in a Mouse Model
title_short Therapeutic Effects of Acer palmatum Thumb. Leaf Extract (KIOM-2015E) on Benzalkonium Chloride-Induced Dry Eye in a Mouse Model
title_sort therapeutic effects of acer palmatum thumb. leaf extract (kiom-2015e) on benzalkonium chloride-induced dry eye in a mouse model
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9736398/
https://www.ncbi.nlm.nih.gov/pubmed/36499298
http://dx.doi.org/10.3390/ijms232314964
work_keys_str_mv AT yimnamhui therapeuticeffectsofacerpalmatumthumbleafextractkiom2015eonbenzalkoniumchlorideinduceddryeyeinamousemodel
AT parkeunhee therapeuticeffectsofacerpalmatumthumbleafextractkiom2015eonbenzalkoniumchlorideinduceddryeyeinamousemodel
AT chowonkyung therapeuticeffectsofacerpalmatumthumbleafextractkiom2015eonbenzalkoniumchlorideinduceddryeyeinamousemodel
AT kimyeounhee therapeuticeffectsofacerpalmatumthumbleafextractkiom2015eonbenzalkoniumchlorideinduceddryeyeinamousemodel
AT majinyeul therapeuticeffectsofacerpalmatumthumbleafextractkiom2015eonbenzalkoniumchlorideinduceddryeyeinamousemodel