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Carbon Dots Derived from Tea Polyphenols as Photosensitizers for Photodynamic Therapy

Photodynamic therapy (PDT) has become an emerging cancer treatment method. Choosing the photosensitizer (PS) compounds is one of the essential factors that can influence the PDT effect and action. Carbon dots (CDs) have shown great potential as photosensitizers in PDT of cancers due to their excelle...

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Autores principales: Yang, Yuxiang, Ding, Haizhen, Li, Zijian, Tedesco, Antonio Claudio, Bi, Hong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9736769/
https://www.ncbi.nlm.nih.gov/pubmed/36500718
http://dx.doi.org/10.3390/molecules27238627
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author Yang, Yuxiang
Ding, Haizhen
Li, Zijian
Tedesco, Antonio Claudio
Bi, Hong
author_facet Yang, Yuxiang
Ding, Haizhen
Li, Zijian
Tedesco, Antonio Claudio
Bi, Hong
author_sort Yang, Yuxiang
collection PubMed
description Photodynamic therapy (PDT) has become an emerging cancer treatment method. Choosing the photosensitizer (PS) compounds is one of the essential factors that can influence the PDT effect and action. Carbon dots (CDs) have shown great potential as photosensitizers in PDT of cancers due to their excellent biocompatibility and high generation of reactive oxygen species (ROS). Here, we used tea polyphenol as raw material for synthesized tea polyphenol carbon dots (T−CDs) that show dual emission bands of red and blue fluorescence and can efficiently generate hydroxyl radicals (OH) under mildly visible irradiation with a LED light (400–500 nm, 15 mW cm(−2)). The extremely low cytotoxicity and excellent biocompatibility of T−CDs without light irradiation were tested using MTT and hemolytic assay. Further, T−CDs have been shown by in vivo experiments, using a mouse breast cancer cell line (4T1) subcutaneously injected in the back of the mouse buttock as a model, to effectively inhibit the tumor cell proliferation in solid tumors and show an excellent PDT effect. In addition, pathological sections of the mice tissues after further treatment showed that the T−CDs had no apparent impact on the major organs of the mice and did not produce any side effect lesions. This work demonstrates that the as−synthesized T−CDs has the potential to be used as a PS in cancer treatment.
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spelling pubmed-97367692022-12-11 Carbon Dots Derived from Tea Polyphenols as Photosensitizers for Photodynamic Therapy Yang, Yuxiang Ding, Haizhen Li, Zijian Tedesco, Antonio Claudio Bi, Hong Molecules Article Photodynamic therapy (PDT) has become an emerging cancer treatment method. Choosing the photosensitizer (PS) compounds is one of the essential factors that can influence the PDT effect and action. Carbon dots (CDs) have shown great potential as photosensitizers in PDT of cancers due to their excellent biocompatibility and high generation of reactive oxygen species (ROS). Here, we used tea polyphenol as raw material for synthesized tea polyphenol carbon dots (T−CDs) that show dual emission bands of red and blue fluorescence and can efficiently generate hydroxyl radicals (OH) under mildly visible irradiation with a LED light (400–500 nm, 15 mW cm(−2)). The extremely low cytotoxicity and excellent biocompatibility of T−CDs without light irradiation were tested using MTT and hemolytic assay. Further, T−CDs have been shown by in vivo experiments, using a mouse breast cancer cell line (4T1) subcutaneously injected in the back of the mouse buttock as a model, to effectively inhibit the tumor cell proliferation in solid tumors and show an excellent PDT effect. In addition, pathological sections of the mice tissues after further treatment showed that the T−CDs had no apparent impact on the major organs of the mice and did not produce any side effect lesions. This work demonstrates that the as−synthesized T−CDs has the potential to be used as a PS in cancer treatment. MDPI 2022-12-06 /pmc/articles/PMC9736769/ /pubmed/36500718 http://dx.doi.org/10.3390/molecules27238627 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Yang, Yuxiang
Ding, Haizhen
Li, Zijian
Tedesco, Antonio Claudio
Bi, Hong
Carbon Dots Derived from Tea Polyphenols as Photosensitizers for Photodynamic Therapy
title Carbon Dots Derived from Tea Polyphenols as Photosensitizers for Photodynamic Therapy
title_full Carbon Dots Derived from Tea Polyphenols as Photosensitizers for Photodynamic Therapy
title_fullStr Carbon Dots Derived from Tea Polyphenols as Photosensitizers for Photodynamic Therapy
title_full_unstemmed Carbon Dots Derived from Tea Polyphenols as Photosensitizers for Photodynamic Therapy
title_short Carbon Dots Derived from Tea Polyphenols as Photosensitizers for Photodynamic Therapy
title_sort carbon dots derived from tea polyphenols as photosensitizers for photodynamic therapy
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9736769/
https://www.ncbi.nlm.nih.gov/pubmed/36500718
http://dx.doi.org/10.3390/molecules27238627
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