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Horizontal transfer of the rfb cluster in Leptospira is a genetic determinant of serovar identity
Leptospira bacteria comprise numerous species, several of which cause serious disease to a broad range of hosts including humans. These spirochetes exhibit large intraspecific variation, resulting in complex tabulations of serogroups/serovars that crisscross the species classification. Serovar ident...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Life Science Alliance LLC
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9736851/ https://www.ncbi.nlm.nih.gov/pubmed/36622346 http://dx.doi.org/10.26508/lsa.202201480 |
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author | Nieves, Cecilia Vincent, Antony T Zarantonelli, Leticia Picardeau, Mathieu Veyrier, Frédéric J Buschiazzo, Alejandro |
author_facet | Nieves, Cecilia Vincent, Antony T Zarantonelli, Leticia Picardeau, Mathieu Veyrier, Frédéric J Buschiazzo, Alejandro |
author_sort | Nieves, Cecilia |
collection | PubMed |
description | Leptospira bacteria comprise numerous species, several of which cause serious disease to a broad range of hosts including humans. These spirochetes exhibit large intraspecific variation, resulting in complex tabulations of serogroups/serovars that crisscross the species classification. Serovar identity, linked to biological/clinical phenotypes, depends on the structure of surface-exposed LPS. Many LPS biosynthesis–encoding genes reside within the chromosomic rfb gene cluster. However, the genetic basis of intraspecies variability is not fully understood, constraining diagnostics/typing methods to cumbersome serologic procedures. We now show that the gene content of the rfb cluster strongly correlates with Leptospira serovar designation. Whole-genome sequencing of pathogenic L. noguchii, including strains of different serogroups, reveals that the rfb cluster undergoes extensive horizontal gene transfer. The rfb clusters from several Leptospira species disclose a univocal correspondence between gene composition and serovar identity. This work paves the way to genetic typing of Leptospira serovars, and to pinpointing specific genes within the distinct rfb clusters, encoding host-specific virulence traits. Further research shall unveil the molecular mechanism of rfb transfer among Leptospira strains and species. |
format | Online Article Text |
id | pubmed-9736851 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Life Science Alliance LLC |
record_format | MEDLINE/PubMed |
spelling | pubmed-97368512022-12-11 Horizontal transfer of the rfb cluster in Leptospira is a genetic determinant of serovar identity Nieves, Cecilia Vincent, Antony T Zarantonelli, Leticia Picardeau, Mathieu Veyrier, Frédéric J Buschiazzo, Alejandro Life Sci Alliance Research Articles Leptospira bacteria comprise numerous species, several of which cause serious disease to a broad range of hosts including humans. These spirochetes exhibit large intraspecific variation, resulting in complex tabulations of serogroups/serovars that crisscross the species classification. Serovar identity, linked to biological/clinical phenotypes, depends on the structure of surface-exposed LPS. Many LPS biosynthesis–encoding genes reside within the chromosomic rfb gene cluster. However, the genetic basis of intraspecies variability is not fully understood, constraining diagnostics/typing methods to cumbersome serologic procedures. We now show that the gene content of the rfb cluster strongly correlates with Leptospira serovar designation. Whole-genome sequencing of pathogenic L. noguchii, including strains of different serogroups, reveals that the rfb cluster undergoes extensive horizontal gene transfer. The rfb clusters from several Leptospira species disclose a univocal correspondence between gene composition and serovar identity. This work paves the way to genetic typing of Leptospira serovars, and to pinpointing specific genes within the distinct rfb clusters, encoding host-specific virulence traits. Further research shall unveil the molecular mechanism of rfb transfer among Leptospira strains and species. Life Science Alliance LLC 2022-12-09 /pmc/articles/PMC9736851/ /pubmed/36622346 http://dx.doi.org/10.26508/lsa.202201480 Text en © 2022 Nieves et al. https://creativecommons.org/licenses/by/4.0/This article is available under a Creative Commons License (Attribution 4.0 International, as described at https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Research Articles Nieves, Cecilia Vincent, Antony T Zarantonelli, Leticia Picardeau, Mathieu Veyrier, Frédéric J Buschiazzo, Alejandro Horizontal transfer of the rfb cluster in Leptospira is a genetic determinant of serovar identity |
title | Horizontal transfer of the rfb cluster in Leptospira is a genetic determinant of serovar identity |
title_full | Horizontal transfer of the rfb cluster in Leptospira is a genetic determinant of serovar identity |
title_fullStr | Horizontal transfer of the rfb cluster in Leptospira is a genetic determinant of serovar identity |
title_full_unstemmed | Horizontal transfer of the rfb cluster in Leptospira is a genetic determinant of serovar identity |
title_short | Horizontal transfer of the rfb cluster in Leptospira is a genetic determinant of serovar identity |
title_sort | horizontal transfer of the rfb cluster in leptospira is a genetic determinant of serovar identity |
topic | Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9736851/ https://www.ncbi.nlm.nih.gov/pubmed/36622346 http://dx.doi.org/10.26508/lsa.202201480 |
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