Cargando…

Design and Validation of a Sensitive Multisteroid LC-MS/MS Assay for the Routine Clinical Use: One-Step Sample Preparation with Phospholipid Removal and Comparison to Immunoassays

Steroid analysis in clinical laboratories is dominated by immunoassays (IAs) that have a high sample turnover but are inherently limited in trueness, precision, and sensitivity. Liquid chromatography coupled to mass spectrometry (LC-MS/MS) has proved to be a far more capable tool, delivering better...

Descripción completa

Detalles Bibliográficos
Autores principales:	Braun, Valentin, Stuppner, Hermann, Risch, Lorenz, Seger, Christoph
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	MDPI 2022
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9736865/ https://www.ncbi.nlm.nih.gov/pubmed/36499017 http://dx.doi.org/10.3390/ijms232314691

Ejemplares similares

Non-Steroidal Drug Interferences in a Quantitative Multisteroid LC-MS/MS Assay
por: Braun, Valentin, et al.
Publicado: (2023)

Multisteroid LC–MS/MS assay for glucocorticoids and androgens and its application in Addison's disease
por: Methlie, Paal, et al.
Publicado: (2013)

The Androstenedione Roche Elecsys immunoassay has superior comparability to the LC-MS/MS assay than the Siemens Immulite immunoassay
por: Wei, Ruhan, et al.
Publicado: (2022)

Hepcidin analysis in pneumonia: Comparison of immunoassay and LC-MS/MS
por: Oppen, Kjersti, et al.
Publicado: (2023)

Cross-Method Comparison of Serum Androstenedione Measurement Using Three Different Assays: The Siemens Immulite Immunoassay, the Roche Elecsys Immunoassay, and an LC/MS-MS Assay
por: Wei, Ruhan, et al.
Publicado: (2021)

Automated LC-MS/MS: Ready for the clinical routine Laboratory?
por: Junger, Sina, et al.
Publicado: (2023)

Analytical bias of automated immunoassays for six serum steroid hormones assessed by LC-MS/MS
por: Debeljak, Željko, et al.
Publicado: (2020)

Optimization of the CYP inhibition assay using LC-MS/MS
por: Abduraman, Muhammad Asyraf, et al.
Publicado: (2022)

Development of an LC–MS/MS Assay and Toxicokinetic Characterization of Hexamethylenetetramine in Rats
por: Kim, Woojin, et al.
Publicado: (2023)

Comparison of Two Immunoassay Screening Methods and a LC-MS/MS in Detecting Traditional and Designer Benzodiazepines in Urine
por: Rossi, Brian, et al.
Publicado: (2021)

Serum thyroglobulin evaluation on LC-MS/MS and immunoassay in TgAb-positive patients with papillary thyroid carcinoma
por: Nishihara, Eijun, et al.
Publicado: (2021)

Determination of hair cortisol in horses: comparison of immunoassay vs LC-HRMS/MS
por: Saluti, Giorgio, et al.
Publicado: (2022)

Identification of lipopeptides in Bacillus megaterium by two-step ultrafiltration and LC–ESI–MS/MS
por: Ma, Yunxiao, et al.
Publicado: (2016)

LC, MS(n) and LC–MS/MS studies for the characterization of degradation products of amlodipine
por: Tiwari, Ravi N., et al.
Publicado: (2015)

Data to support a simultaneous testosterone and estradiol assay in serum by LC-MS/MS
por: Schofield, Ryan C., et al.
Publicado: (2018)

A simple and reliable bile acid assay in human serum by LC‐MS/MS
por: Shen, Ying, et al.
Publicado: (2022)

An Ultrasensitive Routine LC-MS/MS Method for Estradiol and Estrone in the Clinically Relevant Sub-Picomolar Range
por: Bertelsen, Bjørn-Erik, et al.
Publicado: (2020)

Fecal metabolite analysis of Japanese macaques in Yakushima by LC-MS/MS and LC-QTOF-MS
por: TSUCHIDA, Sayaka, et al.
Publicado: (2021)

Protocol for rapid and accurate quantification of phospholipids in yeast and mammalian systems using LC-MS
por: Yang, Sen, et al.
Publicado: (2022)

Development and validation of an LC-MS/MS method by one-step precipitation for cinacalcet in human plasma
por: Lu, Haoyang, et al.
Publicado: (2023)

SAT-355 Comparison of Two Serum Cortisol Immunoassays versus Liquid Chromatography: Tandem Mass Spectrometry (LC-MS/MS)
por: Brito, Luciana, et al.
Publicado: (2019)

Combining HPLC-DAD-QTOF-MS and HPLC-SPE-NMR to Monitor In Vitro Vitetrifolin D Phase I and II Metabolism
por: Sturm, Sonja, et al.
Publicado: (2021)

Largazole Pharmacokinetics in Rats by LC-MS/MS
por: Yu, Mingming, et al.
Publicado: (2014)

Application of LC–MS/MS in the Mycotoxins Studies
por: Gámiz-Gracia, Laura, et al.
Publicado: (2020)

A validated LC-MS/MS assay for quantification of 24(S)-hydroxycholesterol in plasma and cerebrospinal fluid
por: Sidhu, Rohini, et al.
Publicado: (2015)

LC–MS/MS assay for quantitation of enalapril and enalaprilat in plasma for bioequivalence study in Indian subjects
por: Halder, Dhiman, et al.
Publicado: (2017)

HAMA: a multiplexed LC-MS/MS assay for specificity profiling of adenylate-forming enzymes
por: Stanišić, Aleksa, et al.
Publicado: (2019)

Development and validation of a novel LC-MS/MS assay for C-peptide in human serum
por: Owusu, Benjamin Y., et al.
Publicado: (2020)

Determination of N-nitrosodimethylamine in Ranitidine Dosage Forms by ESI-LC-MS/MS; Applications for Routine Laboratory Testing
por: Liu, Jiao, et al.
Publicado: (2021)

Inclusion of 11-Oxygenated Androgens in a Clinical Routine LC-MS/MS Setup for Steroid Hormone Profiling
por: Zeidler, Robert, et al.
Publicado: (2022)

ODP479 Equilibrium Dialysis ID-LC/MS/MS Procedure for Routine Measurement of Free Thyroxine in Serum
por: Zhang, Li, et al.
Publicado: (2022)

LC–MS/MS method for simultaneous quantification of ten antibiotics in human plasma for routine therapeutic drug monitoring
por: Radovanovic, Mirjana, et al.
Publicado: (2022)

Comparison of Flow Injection-MS/MS and LC-MS/MS for the Determination of Ochratoxin A
por: Zhang, Kai
Publicado: (2021)

Trace analysis of emerging and regulated mycotoxins in infant stool by LC-MS/MS
por: Krausová, Magdaléna, et al.
Publicado: (2021)

LC and LC–MS/MS studies for the identification and characterization of degradation products of acebutolol
por: Rakibe, Uday, et al.
Publicado: (2018)

LPPtiger software for lipidome-specific prediction and identification of oxidized phospholipids from LC-MS datasets
por: Ni, Zhixu, et al.
Publicado: (2017)

Comparison of Protein Phosphatase Inhibition Assay with LC-MS/MS for Diagnosis of Microcystin Toxicosis in Veterinary Cases
por: Moore, Caroline E., et al.
Publicado: (2016)

Optimization, validation and application of an assay for the activity of HMG-CoA reductase in vitro by LC–MS/MS()
por: Wang, Jing, et al.
Publicado: (2015)

In vitro assays for characterization of distinct multiple catalytic activities of thyroid peroxidase using LC-MS/MS
por: Tater, Abhishek, et al.
Publicado: (2021)

A data-driven approach for the detection of internal standard outliers in targeted LC-MS/MS assays
por: Wilkes, E.H., et al.
Publicado: (2021)

Cannot write session to /tmp/vufind_sessions/sess_5qm2cftc76bqrcb79ue14o8klf