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The Role of Chitinases in Chronic Airway Inflammation Associated with Tobacco Smoke Exposure

Chitinases and chitinase-like proteins are thought to play a role in innate inflammatory responses. Our study aimed to assess whether chitinase concentration and activity in induced sputum (IS) of patients exposed to tobacco smoke are related to the level of airway inflammation including the level a...

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Autores principales: Przysucha, Natalia, Górska, Katarzyna, Maskey-Warzęchowska, Marta, Proboszcz, Małgorzata, Nejman-Gryz, Patrycja, Paplińska-Goryca, Magdalena, Dymek, Barbara, Zagozdzon, Agnieszka, Krenke, Rafał
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9736934/
https://www.ncbi.nlm.nih.gov/pubmed/36497025
http://dx.doi.org/10.3390/cells11233765
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author Przysucha, Natalia
Górska, Katarzyna
Maskey-Warzęchowska, Marta
Proboszcz, Małgorzata
Nejman-Gryz, Patrycja
Paplińska-Goryca, Magdalena
Dymek, Barbara
Zagozdzon, Agnieszka
Krenke, Rafał
author_facet Przysucha, Natalia
Górska, Katarzyna
Maskey-Warzęchowska, Marta
Proboszcz, Małgorzata
Nejman-Gryz, Patrycja
Paplińska-Goryca, Magdalena
Dymek, Barbara
Zagozdzon, Agnieszka
Krenke, Rafał
author_sort Przysucha, Natalia
collection PubMed
description Chitinases and chitinase-like proteins are thought to play a role in innate inflammatory responses. Our study aimed to assess whether chitinase concentration and activity in induced sputum (IS) of patients exposed to tobacco smoke are related to the level of airway inflammation including the level and activity of chitinases and chitinase-like proteins. The study included 22 patients with chronic obstructive pulmonary disease (COPD), 12 non-COPD smokers, and nine nonsmoking subjects. Sputum CHIT1 and YKL-40 levels and chitinolytic activity were compared with sputum IL-6, IL-8, IL-18, and MMP-9 levels. A hierarchical cluster analysis was also performed. Sputum YKL-40 was higher in COPD patients than in the control groups. Sputum CHIT1 and YKL-40 levels correlated with IS inflammatory cell count as well as with MMP-9 and IL-8 levels. Two main clusters were revealed: Cluster 1 had lower chitinase levels and activity, lower IS macrophage and neutrophil count, and lower IS IL-8, IL-18, and MMP-9 than Cluster 2. Comparison of COPD patients from both clusters revealed significant differences in the IS inflammatory profile despite comparable clinical and functional data. Our findings seem to confirm the involvement of chitinases in smoking-associated chronic airway inflammation and show that airway chitinases may be a potential novel marker in COPD phenotyping.
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spelling pubmed-97369342022-12-11 The Role of Chitinases in Chronic Airway Inflammation Associated with Tobacco Smoke Exposure Przysucha, Natalia Górska, Katarzyna Maskey-Warzęchowska, Marta Proboszcz, Małgorzata Nejman-Gryz, Patrycja Paplińska-Goryca, Magdalena Dymek, Barbara Zagozdzon, Agnieszka Krenke, Rafał Cells Article Chitinases and chitinase-like proteins are thought to play a role in innate inflammatory responses. Our study aimed to assess whether chitinase concentration and activity in induced sputum (IS) of patients exposed to tobacco smoke are related to the level of airway inflammation including the level and activity of chitinases and chitinase-like proteins. The study included 22 patients with chronic obstructive pulmonary disease (COPD), 12 non-COPD smokers, and nine nonsmoking subjects. Sputum CHIT1 and YKL-40 levels and chitinolytic activity were compared with sputum IL-6, IL-8, IL-18, and MMP-9 levels. A hierarchical cluster analysis was also performed. Sputum YKL-40 was higher in COPD patients than in the control groups. Sputum CHIT1 and YKL-40 levels correlated with IS inflammatory cell count as well as with MMP-9 and IL-8 levels. Two main clusters were revealed: Cluster 1 had lower chitinase levels and activity, lower IS macrophage and neutrophil count, and lower IS IL-8, IL-18, and MMP-9 than Cluster 2. Comparison of COPD patients from both clusters revealed significant differences in the IS inflammatory profile despite comparable clinical and functional data. Our findings seem to confirm the involvement of chitinases in smoking-associated chronic airway inflammation and show that airway chitinases may be a potential novel marker in COPD phenotyping. MDPI 2022-11-25 /pmc/articles/PMC9736934/ /pubmed/36497025 http://dx.doi.org/10.3390/cells11233765 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Przysucha, Natalia
Górska, Katarzyna
Maskey-Warzęchowska, Marta
Proboszcz, Małgorzata
Nejman-Gryz, Patrycja
Paplińska-Goryca, Magdalena
Dymek, Barbara
Zagozdzon, Agnieszka
Krenke, Rafał
The Role of Chitinases in Chronic Airway Inflammation Associated with Tobacco Smoke Exposure
title The Role of Chitinases in Chronic Airway Inflammation Associated with Tobacco Smoke Exposure
title_full The Role of Chitinases in Chronic Airway Inflammation Associated with Tobacco Smoke Exposure
title_fullStr The Role of Chitinases in Chronic Airway Inflammation Associated with Tobacco Smoke Exposure
title_full_unstemmed The Role of Chitinases in Chronic Airway Inflammation Associated with Tobacco Smoke Exposure
title_short The Role of Chitinases in Chronic Airway Inflammation Associated with Tobacco Smoke Exposure
title_sort role of chitinases in chronic airway inflammation associated with tobacco smoke exposure
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9736934/
https://www.ncbi.nlm.nih.gov/pubmed/36497025
http://dx.doi.org/10.3390/cells11233765
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