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Drosophila melanogaster Oocytes after Space Flight: The Early Period of Adaptation to the Force of Gravity

The effect of space flight factors and the subsequent adaptation to the Earth’s gravity on oocytes is still poorly understood. Studies of mammalian oocytes in space present significant technical difficulties; therefore, the fruit fly Drosophila melanogaster is a convenient test subject. In this stud...

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Autores principales: Ogneva, Irina V., Golubkova, Maria A., Biryukov, Nikolay S., Kotov, Oleg V.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9736949/
https://www.ncbi.nlm.nih.gov/pubmed/36497128
http://dx.doi.org/10.3390/cells11233871
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author Ogneva, Irina V.
Golubkova, Maria A.
Biryukov, Nikolay S.
Kotov, Oleg V.
author_facet Ogneva, Irina V.
Golubkova, Maria A.
Biryukov, Nikolay S.
Kotov, Oleg V.
author_sort Ogneva, Irina V.
collection PubMed
description The effect of space flight factors and the subsequent adaptation to the Earth’s gravity on oocytes is still poorly understood. Studies of mammalian oocytes in space present significant technical difficulties; therefore, the fruit fly Drosophila melanogaster is a convenient test subject. In this study, we analyzed the structure of the oocytes of the fruit fly Drosophila melanogaster, the maturation of which took place under space flight conditions (the “Cytomehanarium” experiment on the Russian Segment of the ISS during the ISS-67 expedition). The collection of the oocytes began immediately after landing and continued for 12 h. The flies were then transferred onto fresh agar plates and oocyte collection continued for the subsequent 12 h. The stiffness of oocytes was determined by atomic force microscopy and the content of the cytoskeletal proteins by Western blotting. The results demonstrated a significant decrease in the stiffness of oocytes in the flight group compared to the control (26.5 ± 1.1 pN/nm vs. 31.0 ± 1.8 pN/nm) against the background of a decrease in the content of some cytoskeletal proteins involved in the formation of microtubules and microfilaments. This pattern of oocyte structure leads to the disruption of cytokinesis during the cleavage of early embryos.
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spelling pubmed-97369492022-12-11 Drosophila melanogaster Oocytes after Space Flight: The Early Period of Adaptation to the Force of Gravity Ogneva, Irina V. Golubkova, Maria A. Biryukov, Nikolay S. Kotov, Oleg V. Cells Communication The effect of space flight factors and the subsequent adaptation to the Earth’s gravity on oocytes is still poorly understood. Studies of mammalian oocytes in space present significant technical difficulties; therefore, the fruit fly Drosophila melanogaster is a convenient test subject. In this study, we analyzed the structure of the oocytes of the fruit fly Drosophila melanogaster, the maturation of which took place under space flight conditions (the “Cytomehanarium” experiment on the Russian Segment of the ISS during the ISS-67 expedition). The collection of the oocytes began immediately after landing and continued for 12 h. The flies were then transferred onto fresh agar plates and oocyte collection continued for the subsequent 12 h. The stiffness of oocytes was determined by atomic force microscopy and the content of the cytoskeletal proteins by Western blotting. The results demonstrated a significant decrease in the stiffness of oocytes in the flight group compared to the control (26.5 ± 1.1 pN/nm vs. 31.0 ± 1.8 pN/nm) against the background of a decrease in the content of some cytoskeletal proteins involved in the formation of microtubules and microfilaments. This pattern of oocyte structure leads to the disruption of cytokinesis during the cleavage of early embryos. MDPI 2022-12-01 /pmc/articles/PMC9736949/ /pubmed/36497128 http://dx.doi.org/10.3390/cells11233871 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Communication
Ogneva, Irina V.
Golubkova, Maria A.
Biryukov, Nikolay S.
Kotov, Oleg V.
Drosophila melanogaster Oocytes after Space Flight: The Early Period of Adaptation to the Force of Gravity
title Drosophila melanogaster Oocytes after Space Flight: The Early Period of Adaptation to the Force of Gravity
title_full Drosophila melanogaster Oocytes after Space Flight: The Early Period of Adaptation to the Force of Gravity
title_fullStr Drosophila melanogaster Oocytes after Space Flight: The Early Period of Adaptation to the Force of Gravity
title_full_unstemmed Drosophila melanogaster Oocytes after Space Flight: The Early Period of Adaptation to the Force of Gravity
title_short Drosophila melanogaster Oocytes after Space Flight: The Early Period of Adaptation to the Force of Gravity
title_sort drosophila melanogaster oocytes after space flight: the early period of adaptation to the force of gravity
topic Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9736949/
https://www.ncbi.nlm.nih.gov/pubmed/36497128
http://dx.doi.org/10.3390/cells11233871
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