Cargando…

Identification and Functional Characterization of the Transcription Factors AhR/ARNT in Dendroctonus armandi

The aryl hydrocarbon receptor (AhR) and aryl hydrocarbon receptor nuclear translocator (ARNT) belong to the bHLH-PAS (basic Helix–Loop–Helix–Period/ARNT/Single-minded) family of transcription factors, which participate in the sensing and transmitting stimuli of exogenous and endogenous chemical subs...

Descripción completa

Detalles Bibliográficos
Autores principales: Liu, Bin, Chen, Hui
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9736963/
https://www.ncbi.nlm.nih.gov/pubmed/36497113
http://dx.doi.org/10.3390/cells11233856
_version_ 1784847166090510336
author Liu, Bin
Chen, Hui
author_facet Liu, Bin
Chen, Hui
author_sort Liu, Bin
collection PubMed
description The aryl hydrocarbon receptor (AhR) and aryl hydrocarbon receptor nuclear translocator (ARNT) belong to the bHLH-PAS (basic Helix–Loop–Helix–Period/ARNT/Single-minded) family of transcription factors, which participate in the sensing and transmitting stimuli of exogenous and endogenous chemical substances, and subsequently activates genes transcription involved in various detoxification and physiological functions. However, they have not been identified in Dendroctonus armandi, and their roles in the detoxification metabolism are unclear. In the present study, AhR and ARNT of D. armandi were characterized. Spatiotemporal expression profiling indicated that DaAhR and DaARNT were highly expressed in the adult and larval stages of D. armandi and mainly expressed in the midgut and Malpighian tubules of adults. Additionally, the expression of DaAhR and DaARNT significantly increased after exposure to (−)-𝛽-pinene, (+)-3-carene, and (±)-limonene. Silencing DaAhR and DaARNT increased the susceptibility of D. armandi to (−)-𝛽-pinene, (+)-3-carene, and (±)-limonene, and the activities of detoxification enzyme were also remarkably reduced. Moreover, DaCYP6DF1 and DaGSTs2 were significantly down-regulated after injections of dsAhR and dsARNT in the male and female adults, with the expression of DaCYP6DF1 decreasing by higher than 70%. The present study revealed that the transcription factors AhR and ARNT of D. armandi were induced by terpenoids and participated in the regulation of DaCYP6DF1 expression, which was associated with D. armandi’s susceptibility to (−)-𝛽-pinene and (±)-limonene. These results may provide a theoretical basis for the integrated control of D. armandi and improve our comprehension of insect toxicology.
format Online
Article
Text
id pubmed-9736963
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-97369632022-12-11 Identification and Functional Characterization of the Transcription Factors AhR/ARNT in Dendroctonus armandi Liu, Bin Chen, Hui Cells Article The aryl hydrocarbon receptor (AhR) and aryl hydrocarbon receptor nuclear translocator (ARNT) belong to the bHLH-PAS (basic Helix–Loop–Helix–Period/ARNT/Single-minded) family of transcription factors, which participate in the sensing and transmitting stimuli of exogenous and endogenous chemical substances, and subsequently activates genes transcription involved in various detoxification and physiological functions. However, they have not been identified in Dendroctonus armandi, and their roles in the detoxification metabolism are unclear. In the present study, AhR and ARNT of D. armandi were characterized. Spatiotemporal expression profiling indicated that DaAhR and DaARNT were highly expressed in the adult and larval stages of D. armandi and mainly expressed in the midgut and Malpighian tubules of adults. Additionally, the expression of DaAhR and DaARNT significantly increased after exposure to (−)-𝛽-pinene, (+)-3-carene, and (±)-limonene. Silencing DaAhR and DaARNT increased the susceptibility of D. armandi to (−)-𝛽-pinene, (+)-3-carene, and (±)-limonene, and the activities of detoxification enzyme were also remarkably reduced. Moreover, DaCYP6DF1 and DaGSTs2 were significantly down-regulated after injections of dsAhR and dsARNT in the male and female adults, with the expression of DaCYP6DF1 decreasing by higher than 70%. The present study revealed that the transcription factors AhR and ARNT of D. armandi were induced by terpenoids and participated in the regulation of DaCYP6DF1 expression, which was associated with D. armandi’s susceptibility to (−)-𝛽-pinene and (±)-limonene. These results may provide a theoretical basis for the integrated control of D. armandi and improve our comprehension of insect toxicology. MDPI 2022-11-30 /pmc/articles/PMC9736963/ /pubmed/36497113 http://dx.doi.org/10.3390/cells11233856 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Liu, Bin
Chen, Hui
Identification and Functional Characterization of the Transcription Factors AhR/ARNT in Dendroctonus armandi
title Identification and Functional Characterization of the Transcription Factors AhR/ARNT in Dendroctonus armandi
title_full Identification and Functional Characterization of the Transcription Factors AhR/ARNT in Dendroctonus armandi
title_fullStr Identification and Functional Characterization of the Transcription Factors AhR/ARNT in Dendroctonus armandi
title_full_unstemmed Identification and Functional Characterization of the Transcription Factors AhR/ARNT in Dendroctonus armandi
title_short Identification and Functional Characterization of the Transcription Factors AhR/ARNT in Dendroctonus armandi
title_sort identification and functional characterization of the transcription factors ahr/arnt in dendroctonus armandi
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9736963/
https://www.ncbi.nlm.nih.gov/pubmed/36497113
http://dx.doi.org/10.3390/cells11233856
work_keys_str_mv AT liubin identificationandfunctionalcharacterizationofthetranscriptionfactorsahrarntindendroctonusarmandi
AT chenhui identificationandfunctionalcharacterizationofthetranscriptionfactorsahrarntindendroctonusarmandi