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Identification and Functional Characterization of the Transcription Factors AhR/ARNT in Dendroctonus armandi
The aryl hydrocarbon receptor (AhR) and aryl hydrocarbon receptor nuclear translocator (ARNT) belong to the bHLH-PAS (basic Helix–Loop–Helix–Period/ARNT/Single-minded) family of transcription factors, which participate in the sensing and transmitting stimuli of exogenous and endogenous chemical subs...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9736963/ https://www.ncbi.nlm.nih.gov/pubmed/36497113 http://dx.doi.org/10.3390/cells11233856 |
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author | Liu, Bin Chen, Hui |
author_facet | Liu, Bin Chen, Hui |
author_sort | Liu, Bin |
collection | PubMed |
description | The aryl hydrocarbon receptor (AhR) and aryl hydrocarbon receptor nuclear translocator (ARNT) belong to the bHLH-PAS (basic Helix–Loop–Helix–Period/ARNT/Single-minded) family of transcription factors, which participate in the sensing and transmitting stimuli of exogenous and endogenous chemical substances, and subsequently activates genes transcription involved in various detoxification and physiological functions. However, they have not been identified in Dendroctonus armandi, and their roles in the detoxification metabolism are unclear. In the present study, AhR and ARNT of D. armandi were characterized. Spatiotemporal expression profiling indicated that DaAhR and DaARNT were highly expressed in the adult and larval stages of D. armandi and mainly expressed in the midgut and Malpighian tubules of adults. Additionally, the expression of DaAhR and DaARNT significantly increased after exposure to (−)-𝛽-pinene, (+)-3-carene, and (±)-limonene. Silencing DaAhR and DaARNT increased the susceptibility of D. armandi to (−)-𝛽-pinene, (+)-3-carene, and (±)-limonene, and the activities of detoxification enzyme were also remarkably reduced. Moreover, DaCYP6DF1 and DaGSTs2 were significantly down-regulated after injections of dsAhR and dsARNT in the male and female adults, with the expression of DaCYP6DF1 decreasing by higher than 70%. The present study revealed that the transcription factors AhR and ARNT of D. armandi were induced by terpenoids and participated in the regulation of DaCYP6DF1 expression, which was associated with D. armandi’s susceptibility to (−)-𝛽-pinene and (±)-limonene. These results may provide a theoretical basis for the integrated control of D. armandi and improve our comprehension of insect toxicology. |
format | Online Article Text |
id | pubmed-9736963 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-97369632022-12-11 Identification and Functional Characterization of the Transcription Factors AhR/ARNT in Dendroctonus armandi Liu, Bin Chen, Hui Cells Article The aryl hydrocarbon receptor (AhR) and aryl hydrocarbon receptor nuclear translocator (ARNT) belong to the bHLH-PAS (basic Helix–Loop–Helix–Period/ARNT/Single-minded) family of transcription factors, which participate in the sensing and transmitting stimuli of exogenous and endogenous chemical substances, and subsequently activates genes transcription involved in various detoxification and physiological functions. However, they have not been identified in Dendroctonus armandi, and their roles in the detoxification metabolism are unclear. In the present study, AhR and ARNT of D. armandi were characterized. Spatiotemporal expression profiling indicated that DaAhR and DaARNT were highly expressed in the adult and larval stages of D. armandi and mainly expressed in the midgut and Malpighian tubules of adults. Additionally, the expression of DaAhR and DaARNT significantly increased after exposure to (−)-𝛽-pinene, (+)-3-carene, and (±)-limonene. Silencing DaAhR and DaARNT increased the susceptibility of D. armandi to (−)-𝛽-pinene, (+)-3-carene, and (±)-limonene, and the activities of detoxification enzyme were also remarkably reduced. Moreover, DaCYP6DF1 and DaGSTs2 were significantly down-regulated after injections of dsAhR and dsARNT in the male and female adults, with the expression of DaCYP6DF1 decreasing by higher than 70%. The present study revealed that the transcription factors AhR and ARNT of D. armandi were induced by terpenoids and participated in the regulation of DaCYP6DF1 expression, which was associated with D. armandi’s susceptibility to (−)-𝛽-pinene and (±)-limonene. These results may provide a theoretical basis for the integrated control of D. armandi and improve our comprehension of insect toxicology. MDPI 2022-11-30 /pmc/articles/PMC9736963/ /pubmed/36497113 http://dx.doi.org/10.3390/cells11233856 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Liu, Bin Chen, Hui Identification and Functional Characterization of the Transcription Factors AhR/ARNT in Dendroctonus armandi |
title | Identification and Functional Characterization of the Transcription Factors AhR/ARNT in Dendroctonus armandi |
title_full | Identification and Functional Characterization of the Transcription Factors AhR/ARNT in Dendroctonus armandi |
title_fullStr | Identification and Functional Characterization of the Transcription Factors AhR/ARNT in Dendroctonus armandi |
title_full_unstemmed | Identification and Functional Characterization of the Transcription Factors AhR/ARNT in Dendroctonus armandi |
title_short | Identification and Functional Characterization of the Transcription Factors AhR/ARNT in Dendroctonus armandi |
title_sort | identification and functional characterization of the transcription factors ahr/arnt in dendroctonus armandi |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9736963/ https://www.ncbi.nlm.nih.gov/pubmed/36497113 http://dx.doi.org/10.3390/cells11233856 |
work_keys_str_mv | AT liubin identificationandfunctionalcharacterizationofthetranscriptionfactorsahrarntindendroctonusarmandi AT chenhui identificationandfunctionalcharacterizationofthetranscriptionfactorsahrarntindendroctonusarmandi |