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Triacetyl Resveratrol Inhibits PEDV by Inducing the Early Apoptosis In Vitro

PEDV represents an ancient Coronavirus still causing huge economic losses to the porcine breeding industry. Resveratrol has excellent antiviral effects. Triacetyl resveratrol (TCRV), a novel natural derivative of resveratrol, has been recently discovered, and its pharmacological effects need to be e...

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Autores principales: Wang, Xue, Liu, Yi, Li, Kaiyuan, Yang, Ming, Wang, Qingtao, Hao, Zhihui
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9737061/
https://www.ncbi.nlm.nih.gov/pubmed/36498827
http://dx.doi.org/10.3390/ijms232314499
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author Wang, Xue
Liu, Yi
Li, Kaiyuan
Yang, Ming
Wang, Qingtao
Hao, Zhihui
author_facet Wang, Xue
Liu, Yi
Li, Kaiyuan
Yang, Ming
Wang, Qingtao
Hao, Zhihui
author_sort Wang, Xue
collection PubMed
description PEDV represents an ancient Coronavirus still causing huge economic losses to the porcine breeding industry. Resveratrol has excellent antiviral effects. Triacetyl resveratrol (TCRV), a novel natural derivative of resveratrol, has been recently discovered, and its pharmacological effects need to be explored further. This paper aims to explore the relationship between PEDV and TCRV, which offers a novel strategy in the research of antivirals. In our study, Vero cells and IPEC-J2 cells were used as an in vitro model. First, we proved that TCRV had an obvious anti-PEDV effect and a strong inhibitory effect at different time points. Then, we explored the mechanism of inhibition of PEDV infection by TCRV. Our results showed that TCRV could induce the early apoptosis of PEDV-infected cells, in contrast to PEDV-induced apoptosis. Moreover, we observed that TCRV could promote the expression and activation of apoptosis-related proteins and release mitochondrial cytochrome C into cytoplasm. Based on these results, we hypothesized that TCRV induced the early apoptosis of PEDV-infected cells and inhibited PEDV infection by activating the mitochondria-related caspase pathway. Furthermore, we used the inhibitors Z-DEVD-FMK and Pifithrin-α (PFT-α) to support our hypothesis. In conclusion, the TCRV-activated caspase pathway triggered early apoptosis of PEDV-infected cells, thereby inhibiting PEDV infections.
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spelling pubmed-97370612022-12-11 Triacetyl Resveratrol Inhibits PEDV by Inducing the Early Apoptosis In Vitro Wang, Xue Liu, Yi Li, Kaiyuan Yang, Ming Wang, Qingtao Hao, Zhihui Int J Mol Sci Article PEDV represents an ancient Coronavirus still causing huge economic losses to the porcine breeding industry. Resveratrol has excellent antiviral effects. Triacetyl resveratrol (TCRV), a novel natural derivative of resveratrol, has been recently discovered, and its pharmacological effects need to be explored further. This paper aims to explore the relationship between PEDV and TCRV, which offers a novel strategy in the research of antivirals. In our study, Vero cells and IPEC-J2 cells were used as an in vitro model. First, we proved that TCRV had an obvious anti-PEDV effect and a strong inhibitory effect at different time points. Then, we explored the mechanism of inhibition of PEDV infection by TCRV. Our results showed that TCRV could induce the early apoptosis of PEDV-infected cells, in contrast to PEDV-induced apoptosis. Moreover, we observed that TCRV could promote the expression and activation of apoptosis-related proteins and release mitochondrial cytochrome C into cytoplasm. Based on these results, we hypothesized that TCRV induced the early apoptosis of PEDV-infected cells and inhibited PEDV infection by activating the mitochondria-related caspase pathway. Furthermore, we used the inhibitors Z-DEVD-FMK and Pifithrin-α (PFT-α) to support our hypothesis. In conclusion, the TCRV-activated caspase pathway triggered early apoptosis of PEDV-infected cells, thereby inhibiting PEDV infections. MDPI 2022-11-22 /pmc/articles/PMC9737061/ /pubmed/36498827 http://dx.doi.org/10.3390/ijms232314499 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Wang, Xue
Liu, Yi
Li, Kaiyuan
Yang, Ming
Wang, Qingtao
Hao, Zhihui
Triacetyl Resveratrol Inhibits PEDV by Inducing the Early Apoptosis In Vitro
title Triacetyl Resveratrol Inhibits PEDV by Inducing the Early Apoptosis In Vitro
title_full Triacetyl Resveratrol Inhibits PEDV by Inducing the Early Apoptosis In Vitro
title_fullStr Triacetyl Resveratrol Inhibits PEDV by Inducing the Early Apoptosis In Vitro
title_full_unstemmed Triacetyl Resveratrol Inhibits PEDV by Inducing the Early Apoptosis In Vitro
title_short Triacetyl Resveratrol Inhibits PEDV by Inducing the Early Apoptosis In Vitro
title_sort triacetyl resveratrol inhibits pedv by inducing the early apoptosis in vitro
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9737061/
https://www.ncbi.nlm.nih.gov/pubmed/36498827
http://dx.doi.org/10.3390/ijms232314499
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