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Triacetyl Resveratrol Inhibits PEDV by Inducing the Early Apoptosis In Vitro
PEDV represents an ancient Coronavirus still causing huge economic losses to the porcine breeding industry. Resveratrol has excellent antiviral effects. Triacetyl resveratrol (TCRV), a novel natural derivative of resveratrol, has been recently discovered, and its pharmacological effects need to be e...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9737061/ https://www.ncbi.nlm.nih.gov/pubmed/36498827 http://dx.doi.org/10.3390/ijms232314499 |
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author | Wang, Xue Liu, Yi Li, Kaiyuan Yang, Ming Wang, Qingtao Hao, Zhihui |
author_facet | Wang, Xue Liu, Yi Li, Kaiyuan Yang, Ming Wang, Qingtao Hao, Zhihui |
author_sort | Wang, Xue |
collection | PubMed |
description | PEDV represents an ancient Coronavirus still causing huge economic losses to the porcine breeding industry. Resveratrol has excellent antiviral effects. Triacetyl resveratrol (TCRV), a novel natural derivative of resveratrol, has been recently discovered, and its pharmacological effects need to be explored further. This paper aims to explore the relationship between PEDV and TCRV, which offers a novel strategy in the research of antivirals. In our study, Vero cells and IPEC-J2 cells were used as an in vitro model. First, we proved that TCRV had an obvious anti-PEDV effect and a strong inhibitory effect at different time points. Then, we explored the mechanism of inhibition of PEDV infection by TCRV. Our results showed that TCRV could induce the early apoptosis of PEDV-infected cells, in contrast to PEDV-induced apoptosis. Moreover, we observed that TCRV could promote the expression and activation of apoptosis-related proteins and release mitochondrial cytochrome C into cytoplasm. Based on these results, we hypothesized that TCRV induced the early apoptosis of PEDV-infected cells and inhibited PEDV infection by activating the mitochondria-related caspase pathway. Furthermore, we used the inhibitors Z-DEVD-FMK and Pifithrin-α (PFT-α) to support our hypothesis. In conclusion, the TCRV-activated caspase pathway triggered early apoptosis of PEDV-infected cells, thereby inhibiting PEDV infections. |
format | Online Article Text |
id | pubmed-9737061 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-97370612022-12-11 Triacetyl Resveratrol Inhibits PEDV by Inducing the Early Apoptosis In Vitro Wang, Xue Liu, Yi Li, Kaiyuan Yang, Ming Wang, Qingtao Hao, Zhihui Int J Mol Sci Article PEDV represents an ancient Coronavirus still causing huge economic losses to the porcine breeding industry. Resveratrol has excellent antiviral effects. Triacetyl resveratrol (TCRV), a novel natural derivative of resveratrol, has been recently discovered, and its pharmacological effects need to be explored further. This paper aims to explore the relationship between PEDV and TCRV, which offers a novel strategy in the research of antivirals. In our study, Vero cells and IPEC-J2 cells were used as an in vitro model. First, we proved that TCRV had an obvious anti-PEDV effect and a strong inhibitory effect at different time points. Then, we explored the mechanism of inhibition of PEDV infection by TCRV. Our results showed that TCRV could induce the early apoptosis of PEDV-infected cells, in contrast to PEDV-induced apoptosis. Moreover, we observed that TCRV could promote the expression and activation of apoptosis-related proteins and release mitochondrial cytochrome C into cytoplasm. Based on these results, we hypothesized that TCRV induced the early apoptosis of PEDV-infected cells and inhibited PEDV infection by activating the mitochondria-related caspase pathway. Furthermore, we used the inhibitors Z-DEVD-FMK and Pifithrin-α (PFT-α) to support our hypothesis. In conclusion, the TCRV-activated caspase pathway triggered early apoptosis of PEDV-infected cells, thereby inhibiting PEDV infections. MDPI 2022-11-22 /pmc/articles/PMC9737061/ /pubmed/36498827 http://dx.doi.org/10.3390/ijms232314499 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Wang, Xue Liu, Yi Li, Kaiyuan Yang, Ming Wang, Qingtao Hao, Zhihui Triacetyl Resveratrol Inhibits PEDV by Inducing the Early Apoptosis In Vitro |
title | Triacetyl Resveratrol Inhibits PEDV by Inducing the Early Apoptosis In Vitro |
title_full | Triacetyl Resveratrol Inhibits PEDV by Inducing the Early Apoptosis In Vitro |
title_fullStr | Triacetyl Resveratrol Inhibits PEDV by Inducing the Early Apoptosis In Vitro |
title_full_unstemmed | Triacetyl Resveratrol Inhibits PEDV by Inducing the Early Apoptosis In Vitro |
title_short | Triacetyl Resveratrol Inhibits PEDV by Inducing the Early Apoptosis In Vitro |
title_sort | triacetyl resveratrol inhibits pedv by inducing the early apoptosis in vitro |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9737061/ https://www.ncbi.nlm.nih.gov/pubmed/36498827 http://dx.doi.org/10.3390/ijms232314499 |
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