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Mitochondrial DNA as a Candidate Marker of Multiple Organ Failure after Cardiac Surgery

Assess the level of mitochondrial DNA depending on the presence of multiple organ failure in patients after heart surgery. The study included 60 patients who underwent surgical treatment of valvular heart disease using cardiopulmonary bypass. Uncomplicated patients were included in the 1st group (n...

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Autores principales: Grigoriev, Evgeny, Ponasenko, Anastasia V., Sinitskaya, Anna V., Ivkin, Artem A., Kornelyuk, Roman A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9737207/
https://www.ncbi.nlm.nih.gov/pubmed/36499077
http://dx.doi.org/10.3390/ijms232314748
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author Grigoriev, Evgeny
Ponasenko, Anastasia V.
Sinitskaya, Anna V.
Ivkin, Artem A.
Kornelyuk, Roman A.
author_facet Grigoriev, Evgeny
Ponasenko, Anastasia V.
Sinitskaya, Anna V.
Ivkin, Artem A.
Kornelyuk, Roman A.
author_sort Grigoriev, Evgeny
collection PubMed
description Assess the level of mitochondrial DNA depending on the presence of multiple organ failure in patients after heart surgery. The study included 60 patients who underwent surgical treatment of valvular heart disease using cardiopulmonary bypass. Uncomplicated patients were included in the 1st group (n = 30), patients with complications and multiple organ failure (MOF) were included in the 2nd group (n = 30). Serum mtDNA levels were determined by quantitative real-time polymerase chain reaction with fluorescent dyes. Mitochondrial DNA gene expression did not differ between group before surgery. Immediately after the intervention, cytochrome B gene expression was higher in the group with MOF, and it remained high during entire follow-up period. A similar trend was observed in cytochrome oxidase gene expression. Increased NADH levels of gene expressions during the first postoperative day were noted in both groups, the expression showed tendency to increase on the third postoperative day. mtDNA gene expression in the “MOF present” group remained at a higher level compared with the group without complications. A positive correlation was reveled between the severity of MOF according to SOFA score and the level of mtDNA (r = 0.45; p = 0.028) for the end-point “First day”. The ROC analysis showed that mtDNA circulating in plasma (AUC = 0.605) can be a predictor of MOF development. The level of mtDNA significantly increases in case of MOF, irrespective of its cause. (2) The expression of mtDNA genes correlates with the level of MOF severity on the SOFA score.
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spelling pubmed-97372072022-12-11 Mitochondrial DNA as a Candidate Marker of Multiple Organ Failure after Cardiac Surgery Grigoriev, Evgeny Ponasenko, Anastasia V. Sinitskaya, Anna V. Ivkin, Artem A. Kornelyuk, Roman A. Int J Mol Sci Article Assess the level of mitochondrial DNA depending on the presence of multiple organ failure in patients after heart surgery. The study included 60 patients who underwent surgical treatment of valvular heart disease using cardiopulmonary bypass. Uncomplicated patients were included in the 1st group (n = 30), patients with complications and multiple organ failure (MOF) were included in the 2nd group (n = 30). Serum mtDNA levels were determined by quantitative real-time polymerase chain reaction with fluorescent dyes. Mitochondrial DNA gene expression did not differ between group before surgery. Immediately after the intervention, cytochrome B gene expression was higher in the group with MOF, and it remained high during entire follow-up period. A similar trend was observed in cytochrome oxidase gene expression. Increased NADH levels of gene expressions during the first postoperative day were noted in both groups, the expression showed tendency to increase on the third postoperative day. mtDNA gene expression in the “MOF present” group remained at a higher level compared with the group without complications. A positive correlation was reveled between the severity of MOF according to SOFA score and the level of mtDNA (r = 0.45; p = 0.028) for the end-point “First day”. The ROC analysis showed that mtDNA circulating in plasma (AUC = 0.605) can be a predictor of MOF development. The level of mtDNA significantly increases in case of MOF, irrespective of its cause. (2) The expression of mtDNA genes correlates with the level of MOF severity on the SOFA score. MDPI 2022-11-25 /pmc/articles/PMC9737207/ /pubmed/36499077 http://dx.doi.org/10.3390/ijms232314748 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Grigoriev, Evgeny
Ponasenko, Anastasia V.
Sinitskaya, Anna V.
Ivkin, Artem A.
Kornelyuk, Roman A.
Mitochondrial DNA as a Candidate Marker of Multiple Organ Failure after Cardiac Surgery
title Mitochondrial DNA as a Candidate Marker of Multiple Organ Failure after Cardiac Surgery
title_full Mitochondrial DNA as a Candidate Marker of Multiple Organ Failure after Cardiac Surgery
title_fullStr Mitochondrial DNA as a Candidate Marker of Multiple Organ Failure after Cardiac Surgery
title_full_unstemmed Mitochondrial DNA as a Candidate Marker of Multiple Organ Failure after Cardiac Surgery
title_short Mitochondrial DNA as a Candidate Marker of Multiple Organ Failure after Cardiac Surgery
title_sort mitochondrial dna as a candidate marker of multiple organ failure after cardiac surgery
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9737207/
https://www.ncbi.nlm.nih.gov/pubmed/36499077
http://dx.doi.org/10.3390/ijms232314748
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