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Prognostic Value of Histological and Immunohistochemical Data in Diabetic Foot Ulcers

Diabetic foot ulcers are an extremely urgent medical and social problem throughout the world. The purpose of this study was to analyse the histological and immunohistochemical features of tissues and cells of different sections of wounds taken during the primary surgical treatment of chronic wounds...

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Autores principales: Koreyba, Konstantin, Silina, Ekaterina, Tsyplakov, Dmitry, Litvitskiy, Petr, Manturova, Natalia, Balkizov, Zalim, Achar, Raghu Ram, Raju, Nithya Rani, Stupin, Victor
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
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Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9737256/
https://www.ncbi.nlm.nih.gov/pubmed/36498776
http://dx.doi.org/10.3390/jcm11237202
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author Koreyba, Konstantin
Silina, Ekaterina
Tsyplakov, Dmitry
Litvitskiy, Petr
Manturova, Natalia
Balkizov, Zalim
Achar, Raghu Ram
Raju, Nithya Rani
Stupin, Victor
author_facet Koreyba, Konstantin
Silina, Ekaterina
Tsyplakov, Dmitry
Litvitskiy, Petr
Manturova, Natalia
Balkizov, Zalim
Achar, Raghu Ram
Raju, Nithya Rani
Stupin, Victor
author_sort Koreyba, Konstantin
collection PubMed
description Diabetic foot ulcers are an extremely urgent medical and social problem throughout the world. The purpose of this study was to analyse the histological and immunohistochemical features of tissues and cells of different sections of wounds taken during the primary surgical treatment of chronic wounds in patients with diabetic foot syndrome with favourable and unfavourable outcomes. Material and methods. A clinical prospective observational study of the treatment outcomes of fifty-three patients with diabetic foot ulcers hospitalized twice in one specialized centre over the course of the year was conducted. The analysis of histological and immunohistochemical data of the tissues of the edges and the centre of the ulcer taken during the primary surgical treatment was performed. While performing histological analyses of wound tissues, special attention was given to the determination of cellular characteristics of leukocyte-necrotic masses, granulation tissue, and loose and dense connective tissue. Immunohistochemistry was performed using a set of monoclonal antibodies, allowing verification of neutrophilic leukocytes, fibroblasts, and endothelial cells. Results. Unfavourable outcomes (amputation, reamputation, death from cardiovascular diseases, nonhealing ulcer within a year) were registered in 52.8% of cases. Uniform distribution of neutrophils and endothelial cell fibroblasts in all parts of the wound was recorded in patients with a favourable outcome. An unfavourable outcome was predetermined by the uneven content of these cells with a significant increase in neutrophilic leukocytosis in the bottom of the wounds, as well as a significant decrease in the number of fibroblasts and endotheliocytes in the centre of the wounds. Conclusions: The datasets obtained during primary surgical treatment are extremely informative to predict the outcome of the treatment of diabetic foot ulcers and indicate more active surgical strategies with the potential to reduce the treatment time, increase its effectiveness, and eventually make the treatment cost-effective.
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spelling pubmed-97372562022-12-11 Prognostic Value of Histological and Immunohistochemical Data in Diabetic Foot Ulcers Koreyba, Konstantin Silina, Ekaterina Tsyplakov, Dmitry Litvitskiy, Petr Manturova, Natalia Balkizov, Zalim Achar, Raghu Ram Raju, Nithya Rani Stupin, Victor J Clin Med Article Diabetic foot ulcers are an extremely urgent medical and social problem throughout the world. The purpose of this study was to analyse the histological and immunohistochemical features of tissues and cells of different sections of wounds taken during the primary surgical treatment of chronic wounds in patients with diabetic foot syndrome with favourable and unfavourable outcomes. Material and methods. A clinical prospective observational study of the treatment outcomes of fifty-three patients with diabetic foot ulcers hospitalized twice in one specialized centre over the course of the year was conducted. The analysis of histological and immunohistochemical data of the tissues of the edges and the centre of the ulcer taken during the primary surgical treatment was performed. While performing histological analyses of wound tissues, special attention was given to the determination of cellular characteristics of leukocyte-necrotic masses, granulation tissue, and loose and dense connective tissue. Immunohistochemistry was performed using a set of monoclonal antibodies, allowing verification of neutrophilic leukocytes, fibroblasts, and endothelial cells. Results. Unfavourable outcomes (amputation, reamputation, death from cardiovascular diseases, nonhealing ulcer within a year) were registered in 52.8% of cases. Uniform distribution of neutrophils and endothelial cell fibroblasts in all parts of the wound was recorded in patients with a favourable outcome. An unfavourable outcome was predetermined by the uneven content of these cells with a significant increase in neutrophilic leukocytosis in the bottom of the wounds, as well as a significant decrease in the number of fibroblasts and endotheliocytes in the centre of the wounds. Conclusions: The datasets obtained during primary surgical treatment are extremely informative to predict the outcome of the treatment of diabetic foot ulcers and indicate more active surgical strategies with the potential to reduce the treatment time, increase its effectiveness, and eventually make the treatment cost-effective. MDPI 2022-12-04 /pmc/articles/PMC9737256/ /pubmed/36498776 http://dx.doi.org/10.3390/jcm11237202 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Koreyba, Konstantin
Silina, Ekaterina
Tsyplakov, Dmitry
Litvitskiy, Petr
Manturova, Natalia
Balkizov, Zalim
Achar, Raghu Ram
Raju, Nithya Rani
Stupin, Victor
Prognostic Value of Histological and Immunohistochemical Data in Diabetic Foot Ulcers
title Prognostic Value of Histological and Immunohistochemical Data in Diabetic Foot Ulcers
title_full Prognostic Value of Histological and Immunohistochemical Data in Diabetic Foot Ulcers
title_fullStr Prognostic Value of Histological and Immunohistochemical Data in Diabetic Foot Ulcers
title_full_unstemmed Prognostic Value of Histological and Immunohistochemical Data in Diabetic Foot Ulcers
title_short Prognostic Value of Histological and Immunohistochemical Data in Diabetic Foot Ulcers
title_sort prognostic value of histological and immunohistochemical data in diabetic foot ulcers
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9737256/
https://www.ncbi.nlm.nih.gov/pubmed/36498776
http://dx.doi.org/10.3390/jcm11237202
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