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Modeling Movement Disorders via Generation of hiPSC-Derived Motor Neurons

Generation of motor neurons (MNs) from human-induced pluripotent stem cells (hiPSCs) overcomes the limited access to human brain tissues and provides an unprecedent approach for modeling MN-related diseases. In this review, we discuss the recent progression in understanding the regulatory mechanisms...

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Autores principales: Akter, Masuma, Ding, Baojin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9737271/
https://www.ncbi.nlm.nih.gov/pubmed/36497056
http://dx.doi.org/10.3390/cells11233796
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author Akter, Masuma
Ding, Baojin
author_facet Akter, Masuma
Ding, Baojin
author_sort Akter, Masuma
collection PubMed
description Generation of motor neurons (MNs) from human-induced pluripotent stem cells (hiPSCs) overcomes the limited access to human brain tissues and provides an unprecedent approach for modeling MN-related diseases. In this review, we discuss the recent progression in understanding the regulatory mechanisms of MN differentiation and their applications in the generation of MNs from hiPSCs, with a particular focus on two approaches: induction by small molecules and induction by lentiviral delivery of transcription factors. At each induction stage, different culture media and supplements, typical growth conditions and cellular morphology, and specific markers for validation of cell identity and quality control are specifically discussed. Both approaches can generate functional MNs. Currently, the major challenges in modeling neurological diseases using iPSC-derived neurons are: obtaining neurons with high purity and yield; long-term neuron culture to reach full maturation; and how to culture neurons more physiologically to maximize relevance to in vivo conditions.
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spelling pubmed-97372712022-12-11 Modeling Movement Disorders via Generation of hiPSC-Derived Motor Neurons Akter, Masuma Ding, Baojin Cells Review Generation of motor neurons (MNs) from human-induced pluripotent stem cells (hiPSCs) overcomes the limited access to human brain tissues and provides an unprecedent approach for modeling MN-related diseases. In this review, we discuss the recent progression in understanding the regulatory mechanisms of MN differentiation and their applications in the generation of MNs from hiPSCs, with a particular focus on two approaches: induction by small molecules and induction by lentiviral delivery of transcription factors. At each induction stage, different culture media and supplements, typical growth conditions and cellular morphology, and specific markers for validation of cell identity and quality control are specifically discussed. Both approaches can generate functional MNs. Currently, the major challenges in modeling neurological diseases using iPSC-derived neurons are: obtaining neurons with high purity and yield; long-term neuron culture to reach full maturation; and how to culture neurons more physiologically to maximize relevance to in vivo conditions. MDPI 2022-11-27 /pmc/articles/PMC9737271/ /pubmed/36497056 http://dx.doi.org/10.3390/cells11233796 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Review
Akter, Masuma
Ding, Baojin
Modeling Movement Disorders via Generation of hiPSC-Derived Motor Neurons
title Modeling Movement Disorders via Generation of hiPSC-Derived Motor Neurons
title_full Modeling Movement Disorders via Generation of hiPSC-Derived Motor Neurons
title_fullStr Modeling Movement Disorders via Generation of hiPSC-Derived Motor Neurons
title_full_unstemmed Modeling Movement Disorders via Generation of hiPSC-Derived Motor Neurons
title_short Modeling Movement Disorders via Generation of hiPSC-Derived Motor Neurons
title_sort modeling movement disorders via generation of hipsc-derived motor neurons
topic Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9737271/
https://www.ncbi.nlm.nih.gov/pubmed/36497056
http://dx.doi.org/10.3390/cells11233796
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