Transcriptome Analysis of Genes Involved in Fatty Acid and Lipid Biosynthesis in Developing Walnut (Juglans regia L.) Seed Kernels from Qinghai Plateau

Walnut (Juglans regia) is an important woody oil-bearing plant with high nutritional value. For better understanding of the underlying molecular mechanisms of its oil accumulation in the Qinghai Plateau, in this study we monitored walnut fruit development, and 15 cDNA libraries were constructed from walnut seed kernels collected at 72, 79, 93, 118 and 135 days after flowering (DAF). The candidate genes were identified using sequencing and expression analysis. The results showed that the oil content in the kernels increased dramatically in late July and reached the maximum value of 69% in mature seed. More than 90% of the oils were unsaturated fatty acids (UFAs) and linoleic acid (18:2) was the predominant UFA accumulated in mature seed. Differentially expressed genes (DEGs) in 15 KEGG pathways of lipid metabolism were detected. We identified 119 DEGs related to FA de novo biosynthesis (38 DEGs), FA elongation and desaturation (39 DEGs), triacylglycerol (TAG) assembly (24 DEGs), oil bodies (12 DEGs), and transcription factors (TFs, 6 DEGs). The abundantly expressed oleosins, caleosins and steroleosins may be important for timely energy reserve in oil bodies. Weighted gene coexpression network analysis (WGCNA) showed that AP2/ERF and bHLH were the key TFs, and were co-expressed with ACC1, α-CT, BCCP, MAT, KASII, LACS, FATA, and PDCT. Our transcriptome data will enrich public databases and provide new insights into functional genes related to the seed kernel lipid metabolism and oil accumulation in J. regia.