Cargando…
Pseudorogneria libanotica Intraspecific Genetic Polymorphism Revealed by Fluorescence In Situ Hybridization with Newly Identified Tandem Repeats and Wheat Single-Copy Gene Probes
The genus Pseudoroegneria (Nevski) Löve (Triticeae, Poaceae) with its genome abbreviated ‘St’ accounts for more than 60% of perennial Triticeae species. The diploid species Psudoroegneria libanotica (2n = 14) contains the most ancient St genome. Therefore, investigating its chromosomes could provide...
Autores principales: | , , , , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9737853/ https://www.ncbi.nlm.nih.gov/pubmed/36499149 http://dx.doi.org/10.3390/ijms232314818 |
_version_ | 1784847391834243072 |
---|---|
author | Wu, Dandan Yang, Namei Xiang, Qian Zhu, Mingkun Fang, Zhongyan Zheng, Wen Lu, Jiale Sha, Lina Fan, Xing Cheng, Yiran Wang, Yi Kang, Houyang Zhang, Haiqin Zhou, Yonghong |
author_facet | Wu, Dandan Yang, Namei Xiang, Qian Zhu, Mingkun Fang, Zhongyan Zheng, Wen Lu, Jiale Sha, Lina Fan, Xing Cheng, Yiran Wang, Yi Kang, Houyang Zhang, Haiqin Zhou, Yonghong |
author_sort | Wu, Dandan |
collection | PubMed |
description | The genus Pseudoroegneria (Nevski) Löve (Triticeae, Poaceae) with its genome abbreviated ‘St’ accounts for more than 60% of perennial Triticeae species. The diploid species Psudoroegneria libanotica (2n = 14) contains the most ancient St genome. Therefore, investigating its chromosomes could provide some fundamental information required for subsequent studies of St genome evolution. Here, 24 wheat cDNA probes covering seven chromosome groups were mapped in P. libanotica to distinguish homoelogous chromosomes, and newly identified tandem repeats were performed to differentiate seven chromosome pairs. Using these probes, we investigated intraspecific population chromosomal polymorphism of P. libanotica. We found that (i) a duplicated fragment of the 5St long arm was inserted into the short arm of 2St; (ii) asymmetrical fluorescence in situ hybridization (FISH) hybridization signals among 2St, 5St, and 7St homologous chromosome pairs; and (iii) intraspecific population of polymorphism in P. libanotica. These observations established the integrated molecular karyotype of P. libanotica. Moreover, we suggested heterozygosity due to outcrossing habit and adaptation to the local climate of P. libanotica. Specifically, the generated STlib_96 and STlib_98 repeats showed no cross-hybridization signals with wheat chromosomes, suggesting that they are valuable for identifying alien chromosomes or introgressed fragments of wild relatives in wheat. |
format | Online Article Text |
id | pubmed-9737853 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-97378532022-12-11 Pseudorogneria libanotica Intraspecific Genetic Polymorphism Revealed by Fluorescence In Situ Hybridization with Newly Identified Tandem Repeats and Wheat Single-Copy Gene Probes Wu, Dandan Yang, Namei Xiang, Qian Zhu, Mingkun Fang, Zhongyan Zheng, Wen Lu, Jiale Sha, Lina Fan, Xing Cheng, Yiran Wang, Yi Kang, Houyang Zhang, Haiqin Zhou, Yonghong Int J Mol Sci Article The genus Pseudoroegneria (Nevski) Löve (Triticeae, Poaceae) with its genome abbreviated ‘St’ accounts for more than 60% of perennial Triticeae species. The diploid species Psudoroegneria libanotica (2n = 14) contains the most ancient St genome. Therefore, investigating its chromosomes could provide some fundamental information required for subsequent studies of St genome evolution. Here, 24 wheat cDNA probes covering seven chromosome groups were mapped in P. libanotica to distinguish homoelogous chromosomes, and newly identified tandem repeats were performed to differentiate seven chromosome pairs. Using these probes, we investigated intraspecific population chromosomal polymorphism of P. libanotica. We found that (i) a duplicated fragment of the 5St long arm was inserted into the short arm of 2St; (ii) asymmetrical fluorescence in situ hybridization (FISH) hybridization signals among 2St, 5St, and 7St homologous chromosome pairs; and (iii) intraspecific population of polymorphism in P. libanotica. These observations established the integrated molecular karyotype of P. libanotica. Moreover, we suggested heterozygosity due to outcrossing habit and adaptation to the local climate of P. libanotica. Specifically, the generated STlib_96 and STlib_98 repeats showed no cross-hybridization signals with wheat chromosomes, suggesting that they are valuable for identifying alien chromosomes or introgressed fragments of wild relatives in wheat. MDPI 2022-11-26 /pmc/articles/PMC9737853/ /pubmed/36499149 http://dx.doi.org/10.3390/ijms232314818 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Wu, Dandan Yang, Namei Xiang, Qian Zhu, Mingkun Fang, Zhongyan Zheng, Wen Lu, Jiale Sha, Lina Fan, Xing Cheng, Yiran Wang, Yi Kang, Houyang Zhang, Haiqin Zhou, Yonghong Pseudorogneria libanotica Intraspecific Genetic Polymorphism Revealed by Fluorescence In Situ Hybridization with Newly Identified Tandem Repeats and Wheat Single-Copy Gene Probes |
title | Pseudorogneria libanotica Intraspecific Genetic Polymorphism Revealed by Fluorescence In Situ Hybridization with Newly Identified Tandem Repeats and Wheat Single-Copy Gene Probes |
title_full | Pseudorogneria libanotica Intraspecific Genetic Polymorphism Revealed by Fluorescence In Situ Hybridization with Newly Identified Tandem Repeats and Wheat Single-Copy Gene Probes |
title_fullStr | Pseudorogneria libanotica Intraspecific Genetic Polymorphism Revealed by Fluorescence In Situ Hybridization with Newly Identified Tandem Repeats and Wheat Single-Copy Gene Probes |
title_full_unstemmed | Pseudorogneria libanotica Intraspecific Genetic Polymorphism Revealed by Fluorescence In Situ Hybridization with Newly Identified Tandem Repeats and Wheat Single-Copy Gene Probes |
title_short | Pseudorogneria libanotica Intraspecific Genetic Polymorphism Revealed by Fluorescence In Situ Hybridization with Newly Identified Tandem Repeats and Wheat Single-Copy Gene Probes |
title_sort | pseudorogneria libanotica intraspecific genetic polymorphism revealed by fluorescence in situ hybridization with newly identified tandem repeats and wheat single-copy gene probes |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9737853/ https://www.ncbi.nlm.nih.gov/pubmed/36499149 http://dx.doi.org/10.3390/ijms232314818 |
work_keys_str_mv | AT wudandan pseudorognerialibanoticaintraspecificgeneticpolymorphismrevealedbyfluorescenceinsituhybridizationwithnewlyidentifiedtandemrepeatsandwheatsinglecopygeneprobes AT yangnamei pseudorognerialibanoticaintraspecificgeneticpolymorphismrevealedbyfluorescenceinsituhybridizationwithnewlyidentifiedtandemrepeatsandwheatsinglecopygeneprobes AT xiangqian pseudorognerialibanoticaintraspecificgeneticpolymorphismrevealedbyfluorescenceinsituhybridizationwithnewlyidentifiedtandemrepeatsandwheatsinglecopygeneprobes AT zhumingkun pseudorognerialibanoticaintraspecificgeneticpolymorphismrevealedbyfluorescenceinsituhybridizationwithnewlyidentifiedtandemrepeatsandwheatsinglecopygeneprobes AT fangzhongyan pseudorognerialibanoticaintraspecificgeneticpolymorphismrevealedbyfluorescenceinsituhybridizationwithnewlyidentifiedtandemrepeatsandwheatsinglecopygeneprobes AT zhengwen pseudorognerialibanoticaintraspecificgeneticpolymorphismrevealedbyfluorescenceinsituhybridizationwithnewlyidentifiedtandemrepeatsandwheatsinglecopygeneprobes AT lujiale pseudorognerialibanoticaintraspecificgeneticpolymorphismrevealedbyfluorescenceinsituhybridizationwithnewlyidentifiedtandemrepeatsandwheatsinglecopygeneprobes AT shalina pseudorognerialibanoticaintraspecificgeneticpolymorphismrevealedbyfluorescenceinsituhybridizationwithnewlyidentifiedtandemrepeatsandwheatsinglecopygeneprobes AT fanxing pseudorognerialibanoticaintraspecificgeneticpolymorphismrevealedbyfluorescenceinsituhybridizationwithnewlyidentifiedtandemrepeatsandwheatsinglecopygeneprobes AT chengyiran pseudorognerialibanoticaintraspecificgeneticpolymorphismrevealedbyfluorescenceinsituhybridizationwithnewlyidentifiedtandemrepeatsandwheatsinglecopygeneprobes AT wangyi pseudorognerialibanoticaintraspecificgeneticpolymorphismrevealedbyfluorescenceinsituhybridizationwithnewlyidentifiedtandemrepeatsandwheatsinglecopygeneprobes AT kanghouyang pseudorognerialibanoticaintraspecificgeneticpolymorphismrevealedbyfluorescenceinsituhybridizationwithnewlyidentifiedtandemrepeatsandwheatsinglecopygeneprobes AT zhanghaiqin pseudorognerialibanoticaintraspecificgeneticpolymorphismrevealedbyfluorescenceinsituhybridizationwithnewlyidentifiedtandemrepeatsandwheatsinglecopygeneprobes AT zhouyonghong pseudorognerialibanoticaintraspecificgeneticpolymorphismrevealedbyfluorescenceinsituhybridizationwithnewlyidentifiedtandemrepeatsandwheatsinglecopygeneprobes |