Cargando…

Transcriptome Analysis Reveals the Molecular Regularity Mechanism Underlying Stem Bulblet Formation in Oriental Lily ‘Siberia’; Functional Characterization of the LoLOB18 Gene

The formation of underground stem bulblets in lilies is a complex biological process which is key in their micropropagation. Generally, it involves a stem-to-bulblet transition; however, the underlying mechanism remains elusive. It is important to understand the regulatory mechanism of bulblet forma...

Descripción completa

Detalles Bibliográficos
Autores principales: Fang, Shaozhong, Yang, Chenglong, Ali, Muhammad Moaaz, Lin, Mi, Tian, Shengnan, Zhang, Lijuan, Chen, Faxing, Lin, Zhimin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9738039/
https://www.ncbi.nlm.nih.gov/pubmed/36499579
http://dx.doi.org/10.3390/ijms232315246
_version_ 1784847438026113024
author Fang, Shaozhong
Yang, Chenglong
Ali, Muhammad Moaaz
Lin, Mi
Tian, Shengnan
Zhang, Lijuan
Chen, Faxing
Lin, Zhimin
author_facet Fang, Shaozhong
Yang, Chenglong
Ali, Muhammad Moaaz
Lin, Mi
Tian, Shengnan
Zhang, Lijuan
Chen, Faxing
Lin, Zhimin
author_sort Fang, Shaozhong
collection PubMed
description The formation of underground stem bulblets in lilies is a complex biological process which is key in their micropropagation. Generally, it involves a stem-to-bulblet transition; however, the underlying mechanism remains elusive. It is important to understand the regulatory mechanism of bulblet formation for the reproductive efficiency of Lilium. In this study, we investigated the regulatory mechanism of underground stem bulblet formation under different conditions regarding the gravity point angle of the stem, i.e., vertical (control), horizontal, and slanting. The horizontal and slanting group displayed better formation of bulblets in terms of quality and quantity compared with the control group. A transcriptome analysis revealed that sucrose and starch were key energy sources for bulblet formation, auxin and cytokinin likely promoted bulblet formation, and gibberellin inhibited bulblet formation. Based on transcriptome analysis, we identified the LoLOB18 gene, a homolog to AtLOB18, which has been proven to be related to embryogenic development. We established the stem bud growth tissue culture system of Lilium and silenced the LoLOb18 gene using the VIGS system. The results showed that the bulblet induction was reduced with down-regulation of LoLOb18, indicating the involvement of LoLOb18 in stem bulblet formation in lilies. Our research lays a solid foundation for further molecular studies on stem bulblet formation of lilies.
format Online
Article
Text
id pubmed-9738039
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-97380392022-12-11 Transcriptome Analysis Reveals the Molecular Regularity Mechanism Underlying Stem Bulblet Formation in Oriental Lily ‘Siberia’; Functional Characterization of the LoLOB18 Gene Fang, Shaozhong Yang, Chenglong Ali, Muhammad Moaaz Lin, Mi Tian, Shengnan Zhang, Lijuan Chen, Faxing Lin, Zhimin Int J Mol Sci Article The formation of underground stem bulblets in lilies is a complex biological process which is key in their micropropagation. Generally, it involves a stem-to-bulblet transition; however, the underlying mechanism remains elusive. It is important to understand the regulatory mechanism of bulblet formation for the reproductive efficiency of Lilium. In this study, we investigated the regulatory mechanism of underground stem bulblet formation under different conditions regarding the gravity point angle of the stem, i.e., vertical (control), horizontal, and slanting. The horizontal and slanting group displayed better formation of bulblets in terms of quality and quantity compared with the control group. A transcriptome analysis revealed that sucrose and starch were key energy sources for bulblet formation, auxin and cytokinin likely promoted bulblet formation, and gibberellin inhibited bulblet formation. Based on transcriptome analysis, we identified the LoLOB18 gene, a homolog to AtLOB18, which has been proven to be related to embryogenic development. We established the stem bud growth tissue culture system of Lilium and silenced the LoLOb18 gene using the VIGS system. The results showed that the bulblet induction was reduced with down-regulation of LoLOb18, indicating the involvement of LoLOb18 in stem bulblet formation in lilies. Our research lays a solid foundation for further molecular studies on stem bulblet formation of lilies. MDPI 2022-12-03 /pmc/articles/PMC9738039/ /pubmed/36499579 http://dx.doi.org/10.3390/ijms232315246 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Fang, Shaozhong
Yang, Chenglong
Ali, Muhammad Moaaz
Lin, Mi
Tian, Shengnan
Zhang, Lijuan
Chen, Faxing
Lin, Zhimin
Transcriptome Analysis Reveals the Molecular Regularity Mechanism Underlying Stem Bulblet Formation in Oriental Lily ‘Siberia’; Functional Characterization of the LoLOB18 Gene
title Transcriptome Analysis Reveals the Molecular Regularity Mechanism Underlying Stem Bulblet Formation in Oriental Lily ‘Siberia’; Functional Characterization of the LoLOB18 Gene
title_full Transcriptome Analysis Reveals the Molecular Regularity Mechanism Underlying Stem Bulblet Formation in Oriental Lily ‘Siberia’; Functional Characterization of the LoLOB18 Gene
title_fullStr Transcriptome Analysis Reveals the Molecular Regularity Mechanism Underlying Stem Bulblet Formation in Oriental Lily ‘Siberia’; Functional Characterization of the LoLOB18 Gene
title_full_unstemmed Transcriptome Analysis Reveals the Molecular Regularity Mechanism Underlying Stem Bulblet Formation in Oriental Lily ‘Siberia’; Functional Characterization of the LoLOB18 Gene
title_short Transcriptome Analysis Reveals the Molecular Regularity Mechanism Underlying Stem Bulblet Formation in Oriental Lily ‘Siberia’; Functional Characterization of the LoLOB18 Gene
title_sort transcriptome analysis reveals the molecular regularity mechanism underlying stem bulblet formation in oriental lily ‘siberia’; functional characterization of the lolob18 gene
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9738039/
https://www.ncbi.nlm.nih.gov/pubmed/36499579
http://dx.doi.org/10.3390/ijms232315246
work_keys_str_mv AT fangshaozhong transcriptomeanalysisrevealsthemolecularregularitymechanismunderlyingstembulbletformationinorientallilysiberiafunctionalcharacterizationofthelolob18gene
AT yangchenglong transcriptomeanalysisrevealsthemolecularregularitymechanismunderlyingstembulbletformationinorientallilysiberiafunctionalcharacterizationofthelolob18gene
AT alimuhammadmoaaz transcriptomeanalysisrevealsthemolecularregularitymechanismunderlyingstembulbletformationinorientallilysiberiafunctionalcharacterizationofthelolob18gene
AT linmi transcriptomeanalysisrevealsthemolecularregularitymechanismunderlyingstembulbletformationinorientallilysiberiafunctionalcharacterizationofthelolob18gene
AT tianshengnan transcriptomeanalysisrevealsthemolecularregularitymechanismunderlyingstembulbletformationinorientallilysiberiafunctionalcharacterizationofthelolob18gene
AT zhanglijuan transcriptomeanalysisrevealsthemolecularregularitymechanismunderlyingstembulbletformationinorientallilysiberiafunctionalcharacterizationofthelolob18gene
AT chenfaxing transcriptomeanalysisrevealsthemolecularregularitymechanismunderlyingstembulbletformationinorientallilysiberiafunctionalcharacterizationofthelolob18gene
AT linzhimin transcriptomeanalysisrevealsthemolecularregularitymechanismunderlyingstembulbletformationinorientallilysiberiafunctionalcharacterizationofthelolob18gene