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Composition of Fatty Acids and Localization of SREBP1 and ELOVL2 Genes in Cauda Epididymides of Hu Sheep with Different Fertility

SIMPLE SUMMARY: The Chinese Hu sheep is well-known for its high fertility and early sexual maturity. Rams reach sexual maturity at the age of six months. This study investigated differential crude fat content, fatty acid composition and related metabolism genes localization and expression in cauda e...

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Autores principales: Liu, Jiamei, Li, Wanhong, Weng, Xiuxiu, Yue, Xiangpeng, Li, Fadi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9738327/
https://www.ncbi.nlm.nih.gov/pubmed/36496823
http://dx.doi.org/10.3390/ani12233302
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author Liu, Jiamei
Li, Wanhong
Weng, Xiuxiu
Yue, Xiangpeng
Li, Fadi
author_facet Liu, Jiamei
Li, Wanhong
Weng, Xiuxiu
Yue, Xiangpeng
Li, Fadi
author_sort Liu, Jiamei
collection PubMed
description SIMPLE SUMMARY: The Chinese Hu sheep is well-known for its high fertility and early sexual maturity. Rams reach sexual maturity at the age of six months. This study investigated differential crude fat content, fatty acid composition and related metabolism genes localization and expression in cauda epididymis between high and low fertility Hu sheep at six months old. The crude fat content was positively correlated with epididymal weight. High expression of SREBP1 and EVOVL2 in high fertility Hu sheep may lead to the higher n-3 PUFA content, especially DHA, providing a basis for understanding the relationship between fertility and sperm storage environment. ABSTRACT: The epididymis is an organ that transports, matures and stores sperm, and has functions such as secretion and absorption. Polyunsaturated fatty acid (PUFA) compositions in sperm membrane were changed during the process of epididymis maturation and influence the male fertility. This study aimed to investigate differences in crude fat and fatty acid content in cauda epididymis between high and low fertility of Hu sheep. One hundred and seventy-nine Hu ram lambs were fed from 56 days to 6 months under the same environment. After the feeding trial, all rams were slaughtered, and the body weight, testicular weight, epididymal weight and sperm density were measured. Pearson correlation analysis showed significantly moderate positive correlation between epididymal weight and sperm density and testicular weight. Eighteen rams were selected and divided into the high fertility group (H, n = 9) and low fertility group (L, n = 9) according to the epididymal weight, sperm density and histomorphology. The crude fat content, fatty acid profile and genes related to fatty acid metabolism were detected. The crude fat content, total fatty acid, total n-3 PUFA and docosahexaenoic acid (C22:6n-3, DHA) content of cauda epididymis in high fertility group was significantly higher than those in low fertility group (p < 0.05). However, the ratio of n-6/n-3 PUFA was significantly lower than that in group L (p < 0.05). Immunohistochemistry results showed that SREBP1 and ELOVL2 were expressed in pseudostratified columnar ciliated epithelium and smooth muscle cells. The mRNA expression of SREBP1 (p = 0.09) and ELOVL2 (p < 0.05) in the high fertility group were increased. In conclusion, the high expression of SREBP1 and ELOVL2 may contribute to high n-3 PUFA content in cauda epididymis of high-fertility Hu sheep.
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spelling pubmed-97383272022-12-11 Composition of Fatty Acids and Localization of SREBP1 and ELOVL2 Genes in Cauda Epididymides of Hu Sheep with Different Fertility Liu, Jiamei Li, Wanhong Weng, Xiuxiu Yue, Xiangpeng Li, Fadi Animals (Basel) Article SIMPLE SUMMARY: The Chinese Hu sheep is well-known for its high fertility and early sexual maturity. Rams reach sexual maturity at the age of six months. This study investigated differential crude fat content, fatty acid composition and related metabolism genes localization and expression in cauda epididymis between high and low fertility Hu sheep at six months old. The crude fat content was positively correlated with epididymal weight. High expression of SREBP1 and EVOVL2 in high fertility Hu sheep may lead to the higher n-3 PUFA content, especially DHA, providing a basis for understanding the relationship between fertility and sperm storage environment. ABSTRACT: The epididymis is an organ that transports, matures and stores sperm, and has functions such as secretion and absorption. Polyunsaturated fatty acid (PUFA) compositions in sperm membrane were changed during the process of epididymis maturation and influence the male fertility. This study aimed to investigate differences in crude fat and fatty acid content in cauda epididymis between high and low fertility of Hu sheep. One hundred and seventy-nine Hu ram lambs were fed from 56 days to 6 months under the same environment. After the feeding trial, all rams were slaughtered, and the body weight, testicular weight, epididymal weight and sperm density were measured. Pearson correlation analysis showed significantly moderate positive correlation between epididymal weight and sperm density and testicular weight. Eighteen rams were selected and divided into the high fertility group (H, n = 9) and low fertility group (L, n = 9) according to the epididymal weight, sperm density and histomorphology. The crude fat content, fatty acid profile and genes related to fatty acid metabolism were detected. The crude fat content, total fatty acid, total n-3 PUFA and docosahexaenoic acid (C22:6n-3, DHA) content of cauda epididymis in high fertility group was significantly higher than those in low fertility group (p < 0.05). However, the ratio of n-6/n-3 PUFA was significantly lower than that in group L (p < 0.05). Immunohistochemistry results showed that SREBP1 and ELOVL2 were expressed in pseudostratified columnar ciliated epithelium and smooth muscle cells. The mRNA expression of SREBP1 (p = 0.09) and ELOVL2 (p < 0.05) in the high fertility group were increased. In conclusion, the high expression of SREBP1 and ELOVL2 may contribute to high n-3 PUFA content in cauda epididymis of high-fertility Hu sheep. MDPI 2022-11-26 /pmc/articles/PMC9738327/ /pubmed/36496823 http://dx.doi.org/10.3390/ani12233302 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Liu, Jiamei
Li, Wanhong
Weng, Xiuxiu
Yue, Xiangpeng
Li, Fadi
Composition of Fatty Acids and Localization of SREBP1 and ELOVL2 Genes in Cauda Epididymides of Hu Sheep with Different Fertility
title Composition of Fatty Acids and Localization of SREBP1 and ELOVL2 Genes in Cauda Epididymides of Hu Sheep with Different Fertility
title_full Composition of Fatty Acids and Localization of SREBP1 and ELOVL2 Genes in Cauda Epididymides of Hu Sheep with Different Fertility
title_fullStr Composition of Fatty Acids and Localization of SREBP1 and ELOVL2 Genes in Cauda Epididymides of Hu Sheep with Different Fertility
title_full_unstemmed Composition of Fatty Acids and Localization of SREBP1 and ELOVL2 Genes in Cauda Epididymides of Hu Sheep with Different Fertility
title_short Composition of Fatty Acids and Localization of SREBP1 and ELOVL2 Genes in Cauda Epididymides of Hu Sheep with Different Fertility
title_sort composition of fatty acids and localization of srebp1 and elovl2 genes in cauda epididymides of hu sheep with different fertility
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9738327/
https://www.ncbi.nlm.nih.gov/pubmed/36496823
http://dx.doi.org/10.3390/ani12233302
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