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MAPLE Processed Nanostructures for Antimicrobial Coatings

Despite their great benefits for debilitated patients, indwelling devices are prone to become easily colonized by resident and opportunistic microorganisms, which have the ability to attach to their surfaces and form highly specialized communities called biofilms. These are extremely resistant to ho...

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Autores principales: Hudiță, Ariana, Grumezescu, Valentina, Gherasim, Oana, Grumezescu, Alexandru Mihai, Dorcioman, Gabriela, Negut, Irina, Oprea, Ovidiu-Cristian, Vasile, Bogdan Ștefan, Gălățeanu, Bianca, Curuțiu, Carmen, Holban, Alina Maria
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9738358/
https://www.ncbi.nlm.nih.gov/pubmed/36499682
http://dx.doi.org/10.3390/ijms232315355
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author Hudiță, Ariana
Grumezescu, Valentina
Gherasim, Oana
Grumezescu, Alexandru Mihai
Dorcioman, Gabriela
Negut, Irina
Oprea, Ovidiu-Cristian
Vasile, Bogdan Ștefan
Gălățeanu, Bianca
Curuțiu, Carmen
Holban, Alina Maria
author_facet Hudiță, Ariana
Grumezescu, Valentina
Gherasim, Oana
Grumezescu, Alexandru Mihai
Dorcioman, Gabriela
Negut, Irina
Oprea, Ovidiu-Cristian
Vasile, Bogdan Ștefan
Gălățeanu, Bianca
Curuțiu, Carmen
Holban, Alina Maria
author_sort Hudiță, Ariana
collection PubMed
description Despite their great benefits for debilitated patients, indwelling devices are prone to become easily colonized by resident and opportunistic microorganisms, which have the ability to attach to their surfaces and form highly specialized communities called biofilms. These are extremely resistant to host defense mechanisms and antibiotics, leading to treatment failure and device replacement, but also to life-threatening complications. In this study, we aimed to optimize a silica (SiO(2))-coated magnetite (Fe(3)O(4))-based nanosystem containing the natural antimicrobial agent, eugenol (E), suitable for MAPLE (matrix-assisted pulsed laser evaporation) deposition as a bioactive coating for biomedical applications. X-ray diffraction, thermogravimetric analysis, Fourier-transform infrared spectroscopy, and transmission electron microscopy investigations were employed to characterize the obtained nanosystems. The in vitro tests evidenced the superior biocompatibility of such nanostructured coatings, as revealed by their non-cytotoxic activity and ability to promote cellular proliferation and sustain normal cellular development of dermal fibroblasts. Moreover, the obtained nanocoatings did not induce proinflammatory events in human blood samples. Our studies demonstrated that Fe(3)O(4) NPs can improve the antimicrobial activity of E, while the use of a SiO(2) matrix may increase its efficiency over prolonged periods of time. The Fe(3)O(4)@SiO(2) nanosystems showed excellent biocompatibility, sustaining human dermal fibroblasts’ viability, proliferation, and typical architecture. More, the novel coatings lack proinflammatory potential as revealed by the absence of proinflammatory cytokine expression in response to human blood sample interactions.
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spelling pubmed-97383582022-12-11 MAPLE Processed Nanostructures for Antimicrobial Coatings Hudiță, Ariana Grumezescu, Valentina Gherasim, Oana Grumezescu, Alexandru Mihai Dorcioman, Gabriela Negut, Irina Oprea, Ovidiu-Cristian Vasile, Bogdan Ștefan Gălățeanu, Bianca Curuțiu, Carmen Holban, Alina Maria Int J Mol Sci Article Despite their great benefits for debilitated patients, indwelling devices are prone to become easily colonized by resident and opportunistic microorganisms, which have the ability to attach to their surfaces and form highly specialized communities called biofilms. These are extremely resistant to host defense mechanisms and antibiotics, leading to treatment failure and device replacement, but also to life-threatening complications. In this study, we aimed to optimize a silica (SiO(2))-coated magnetite (Fe(3)O(4))-based nanosystem containing the natural antimicrobial agent, eugenol (E), suitable for MAPLE (matrix-assisted pulsed laser evaporation) deposition as a bioactive coating for biomedical applications. X-ray diffraction, thermogravimetric analysis, Fourier-transform infrared spectroscopy, and transmission electron microscopy investigations were employed to characterize the obtained nanosystems. The in vitro tests evidenced the superior biocompatibility of such nanostructured coatings, as revealed by their non-cytotoxic activity and ability to promote cellular proliferation and sustain normal cellular development of dermal fibroblasts. Moreover, the obtained nanocoatings did not induce proinflammatory events in human blood samples. Our studies demonstrated that Fe(3)O(4) NPs can improve the antimicrobial activity of E, while the use of a SiO(2) matrix may increase its efficiency over prolonged periods of time. The Fe(3)O(4)@SiO(2) nanosystems showed excellent biocompatibility, sustaining human dermal fibroblasts’ viability, proliferation, and typical architecture. More, the novel coatings lack proinflammatory potential as revealed by the absence of proinflammatory cytokine expression in response to human blood sample interactions. MDPI 2022-12-05 /pmc/articles/PMC9738358/ /pubmed/36499682 http://dx.doi.org/10.3390/ijms232315355 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Hudiță, Ariana
Grumezescu, Valentina
Gherasim, Oana
Grumezescu, Alexandru Mihai
Dorcioman, Gabriela
Negut, Irina
Oprea, Ovidiu-Cristian
Vasile, Bogdan Ștefan
Gălățeanu, Bianca
Curuțiu, Carmen
Holban, Alina Maria
MAPLE Processed Nanostructures for Antimicrobial Coatings
title MAPLE Processed Nanostructures for Antimicrobial Coatings
title_full MAPLE Processed Nanostructures for Antimicrobial Coatings
title_fullStr MAPLE Processed Nanostructures for Antimicrobial Coatings
title_full_unstemmed MAPLE Processed Nanostructures for Antimicrobial Coatings
title_short MAPLE Processed Nanostructures for Antimicrobial Coatings
title_sort maple processed nanostructures for antimicrobial coatings
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9738358/
https://www.ncbi.nlm.nih.gov/pubmed/36499682
http://dx.doi.org/10.3390/ijms232315355
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